Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1069 and GFF1070 are separated by 63 nucleotides GFF1070 and GFF1071 are separated by 58 nucleotides GFF1071 and GFF1072 are separated by 70 nucleotides GFF1072 and GFF1073 are separated by 140 nucleotides
GFF1069 - hypothetical protein, at 382,400 to 383,335
GFF1069
GFF1070 - no description, at 383,399 to 383,665
GFF1070
GFF1071 - no description, at 383,724 to 384,020
GFF1071
GFF1072 - no description, at 384,091 to 384,279
GFF1072
GFF1073 - Glutathione S-transferase (EC 2.5.1.18), at 384,420 to 385,124
GFF1073
Position (kb)
384
385 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 383.107 kb on + strand, within GFF1069 at 383.108 kb on - strand, within GFF1069 at 383.350 kb on + strand at 383.350 kb on + strand at 383.351 kb on - strand at 383.413 kb on + strand at 383.413 kb on + strand at 383.413 kb on + strand at 383.414 kb on - strand at 383.414 kb on - strand at 383.414 kb on - strand at 383.414 kb on - strand at 383.414 kb on - strand at 383.414 kb on - strand at 383.414 kb on - strand at 383.414 kb on - strand at 383.414 kb on - strand at 383.419 kb on + strand at 383.419 kb on + strand at 383.419 kb on + strand at 383.419 kb on + strand at 383.419 kb on + strand at 383.420 kb on - strand at 383.420 kb on - strand at 383.420 kb on - strand at 383.420 kb on - strand at 383.457 kb on - strand, within GFF1070 at 383.457 kb on - strand, within GFF1070 at 383.537 kb on + strand, within GFF1070 at 383.537 kb on + strand, within GFF1070 at 383.537 kb on + strand, within GFF1070 at 383.537 kb on + strand, within GFF1070 at 383.537 kb on + strand, within GFF1070 at 383.537 kb on + strand, within GFF1070 at 383.538 kb on - strand, within GFF1070 at 383.561 kb on + strand, within GFF1070 at 383.562 kb on - strand, within GFF1070 at 383.562 kb on - strand, within GFF1070 at 383.562 kb on - strand, within GFF1070 at 383.562 kb on - strand, within GFF1070 at 383.685 kb on + strand at 383.685 kb on + strand at 383.685 kb on + strand at 383.685 kb on + strand at 383.685 kb on + strand at 383.685 kb on + strand at 383.685 kb on + strand at 383.686 kb on - strand at 383.686 kb on - strand at 383.686 kb on - strand at 383.760 kb on + strand, within GFF1071 at 383.760 kb on + strand, within GFF1071 at 383.760 kb on + strand, within GFF1071 at 383.760 kb on + strand, within GFF1071 at 383.761 kb on - strand, within GFF1071 at 383.761 kb on - strand, within GFF1071 at 383.857 kb on - strand, within GFF1071 at 383.857 kb on - strand, within GFF1071 at 383.916 kb on - strand, within GFF1071 at 383.937 kb on + strand, within GFF1071 at 383.937 kb on + strand, within GFF1071 at 383.937 kb on + strand, within GFF1071 at 383.937 kb on + strand, within GFF1071 at 383.937 kb on + strand, within GFF1071 at 383.938 kb on - strand, within GFF1071 at 383.938 kb on - strand, within GFF1071 at 384.056 kb on + strand at 384.056 kb on + strand at 384.056 kb on + strand at 384.056 kb on + strand at 384.057 kb on - strand at 384.057 kb on - strand at 384.057 kb on - strand at 384.057 kb on - strand at 384.057 kb on - strand at 384.102 kb on + strand at 384.217 kb on + strand, within GFF1072 at 384.218 kb on - strand, within GFF1072 at 384.218 kb on - strand, within GFF1072 at 384.265 kb on + strand at 384.266 kb on - strand at 384.375 kb on + strand at 384.375 kb on + strand at 384.375 kb on + strand at 384.375 kb on + strand at 384.375 kb on + strand at 384.375 kb on + strand at 384.375 kb on + strand at 384.375 kb on + strand at 384.375 kb on + strand at 384.375 kb on + strand at 384.375 kb on + strand at 384.375 kb on + strand at 384.375 kb on + strand at 384.375 kb on + strand at 384.375 kb on + strand at 384.376 kb on - strand at 384.376 kb on - strand at 384.376 kb on - strand at 384.376 kb on - strand at 384.376 kb on - strand at 384.376 kb on - strand at 384.376 kb on - strand at 384.376 kb on - strand at 384.376 kb on - strand at 384.376 kb on - strand at 384.376 kb on - strand at 384.376 kb on - strand at 384.376 kb on - strand at 384.376 kb on - strand at 384.376 kb on - strand at 384.376 kb on - strand at 384.376 kb on - strand at 384.376 kb on - strand at 384.376 kb on - strand at 384.376 kb on - strand at 384.376 kb on - strand at 384.376 kb on - strand at 384.376 kb on - strand at 384.377 kb on + strand at 384.377 kb on + strand at 384.377 kb on + strand at 384.378 kb on - strand at 384.378 kb on - strand at 384.378 kb on - strand at 384.378 kb on - strand at 384.378 kb on - strand at 384.378 kb on - strand at 384.378 kb on - strand at 384.378 kb on - strand at 384.378 kb on - strand at 384.378 kb on - strand at 384.378 kb on - strand at 384.378 kb on - strand at 384.444 kb on + strand at 384.444 kb on + strand at 384.444 kb on + strand at 384.445 kb on - strand at 384.445 kb on - strand at 384.445 kb on - strand at 384.507 kb on + strand, within GFF1073 at 384.660 kb on + strand, within GFF1073 at 384.660 kb on + strand, within GFF1073 at 384.661 kb on - strand, within GFF1073 at 384.789 kb on + strand, within GFF1073 at 384.789 kb on + strand, within GFF1073 at 384.789 kb on + strand, within GFF1073 at 384.789 kb on + strand, within GFF1073 at 384.789 kb on + strand, within GFF1073 at 384.789 kb on + strand, within GFF1073 at 384.789 kb on + strand, within GFF1073 at 384.789 kb on + strand, within GFF1073 at 384.789 kb on + strand, within GFF1073 at 384.789 kb on + strand, within GFF1073 at 384.790 kb on - strand, within GFF1073 at 384.790 kb on - strand, within GFF1073 at 384.790 kb on - strand, within GFF1073 at 384.790 kb on - strand, within GFF1073 at 384.790 kb on - strand, within GFF1073 at 384.790 kb on - strand, within GFF1073 at 384.790 kb on - strand, within GFF1073 at 384.790 kb on - strand, within GFF1073 at 384.790 kb on - strand, within GFF1073 at 384.810 kb on + strand, within GFF1073 at 384.811 kb on - strand, within GFF1073 at 384.811 kb on - strand, within GFF1073 at 384.849 kb on + strand, within GFF1073 at 384.849 kb on + strand, within GFF1073 at 384.849 kb on + strand, within GFF1073 at 384.849 kb on + strand, within GFF1073 at 384.849 kb on + strand, within GFF1073 at 384.849 kb on + strand, within GFF1073 at 384.849 kb on + strand, within GFF1073 at 384.849 kb on + strand, within GFF1073 at 384.849 kb on + strand, within GFF1073 at 384.849 kb on + strand, within GFF1073 at 384.850 kb on - strand, within GFF1073 at 384.850 kb on - strand, within GFF1073 at 384.850 kb on - strand, within GFF1073 at 384.850 kb on - strand, within GFF1073 at 384.850 kb on - strand, within GFF1073 at 384.850 kb on - strand, within GFF1073 at 384.903 kb on + strand, within GFF1073 at 384.903 kb on + strand, within GFF1073 at 384.903 kb on + strand, within GFF1073 at 384.903 kb on + strand, within GFF1073 at 384.903 kb on + strand, within GFF1073 at 384.904 kb on - strand, within GFF1073 at 385.111 kb on - strand at 385.161 kb on + strand at 385.161 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 383,107 + GFF1069 0.76 -0.6 383,108 - GFF1069 0.76 +0.2 383,350 + +0.1 383,350 + -0.2 383,351 - -0.1 383,413 + +0.3 383,413 + -0.0 383,413 + -2.1 383,414 - -1.3 383,414 - -0.9 383,414 - +0.1 383,414 - -0.7 383,414 - +1.2 383,414 - +0.3 383,414 - -0.5 383,414 - -1.3 383,414 - -0.1 383,419 + -0.2 383,419 + -0.2 383,419 + +1.1 383,419 + +0.2 383,419 + -1.1 383,420 - +0.5 383,420 - -0.5 383,420 - -0.1 383,420 - +0.2 383,457 - GFF1070 0.22 -0.8 383,457 - GFF1070 0.22 -0.2 383,537 + GFF1070 0.52 +0.3 383,537 + GFF1070 0.52 -0.7 383,537 + GFF1070 0.52 -1.3 383,537 + GFF1070 0.52 -0.5 383,537 + GFF1070 0.52 -0.7 383,537 + GFF1070 0.52 +0.0 383,538 - GFF1070 0.52 -2.1 383,561 + GFF1070 0.61 +0.0 383,562 - GFF1070 0.61 -0.2 383,562 - GFF1070 0.61 +0.6 383,562 - GFF1070 0.61 -1.3 383,562 - GFF1070 0.61 -0.8 383,685 + +0.4 383,685 + -0.2 383,685 + -0.5 383,685 + -0.1 383,685 + -1.2 383,685 + +1.5 383,685 + -0.1 383,686 - -0.9 383,686 - -1.7 383,686 - -0.4 383,760 + GFF1071 0.12 -0.1 383,760 + GFF1071 0.12 -0.4 383,760 + GFF1071 0.12 -0.4 383,760 + GFF1071 0.12 +0.1 383,761 - GFF1071 0.12 -0.6 383,761 - GFF1071 0.12 -0.6 383,857 - GFF1071 0.45 +0.6 383,857 - GFF1071 0.45 +0.1 383,916 - GFF1071 0.65 +0.5 383,937 + GFF1071 0.72 -0.6 383,937 + GFF1071 0.72 +0.4 383,937 + GFF1071 0.72 +0.3 383,937 + GFF1071 0.72 +0.6 383,937 + GFF1071 0.72 -0.4 383,938 - GFF1071 0.72 +0.8 383,938 - GFF1071 0.72 +1.0 384,056 + +2.9 384,056 + -1.3 384,056 + -0.4 384,056 + -1.1 384,057 - +0.3 384,057 - -0.1 384,057 - -0.2 384,057 - -0.4 384,057 - -1.3 384,102 + -0.7 384,217 + GFF1072 0.67 -1.0 384,218 - GFF1072 0.67 -1.3 384,218 - GFF1072 0.67 -1.3 384,265 + -1.1 384,266 - +0.1 384,375 + -0.2 384,375 + -0.1 384,375 + -0.6 384,375 + -0.8 384,375 + +0.0 384,375 + -0.9 384,375 + +0.7 384,375 + -1.5 384,375 + -1.8 384,375 + +0.5 384,375 + -0.6 384,375 + -0.2 384,375 + +1.1 384,375 + -0.2 384,375 + -0.2 384,376 - -0.1 384,376 - +0.1 384,376 - -0.9 384,376 - +0.3 384,376 - -0.6 384,376 - +1.5 384,376 - -1.1 384,376 - -1.2 384,376 - -0.8 384,376 - +0.2 384,376 - +1.5 384,376 - -1.8 384,376 - -0.6 384,376 - +0.5 384,376 - -0.7 384,376 - -0.7 384,376 - -1.5 384,376 - -0.8 384,376 - -0.6 384,376 - -1.3 384,376 - +0.4 384,376 - -0.9 384,376 - -0.6 384,377 + -0.8 384,377 + -0.6 384,377 + +0.3 384,378 - -1.5 384,378 - -0.1 384,378 - -1.8 384,378 - -0.3 384,378 - -1.2 384,378 - -0.8 384,378 - -0.3 384,378 - -1.1 384,378 - +0.2 384,378 - -0.5 384,378 - -0.2 384,378 - +0.6 384,444 + +1.2 384,444 + +0.3 384,444 + -0.3 384,445 - -0.3 384,445 - -1.0 384,445 - +0.1 384,507 + GFF1073 0.12 -0.1 384,660 + GFF1073 0.34 -0.1 384,660 + GFF1073 0.34 -0.8 384,661 - GFF1073 0.34 -0.4 384,789 + GFF1073 0.52 +2.0 384,789 + GFF1073 0.52 -0.3 384,789 + GFF1073 0.52 -0.5 384,789 + GFF1073 0.52 +0.5 384,789 + GFF1073 0.52 +0.5 384,789 + GFF1073 0.52 -0.8 384,789 + GFF1073 0.52 +0.5 384,789 + GFF1073 0.52 -0.4 384,789 + GFF1073 0.52 -0.2 384,789 + GFF1073 0.52 +0.7 384,790 - GFF1073 0.52 +0.4 384,790 - GFF1073 0.52 +0.3 384,790 - GFF1073 0.52 +0.6 384,790 - GFF1073 0.52 +0.8 384,790 - GFF1073 0.52 +0.1 384,790 - GFF1073 0.52 -0.0 384,790 - GFF1073 0.52 -1.5 384,790 - GFF1073 0.52 -0.1 384,790 - GFF1073 0.52 -1.8 384,810 + GFF1073 0.55 -0.7 384,811 - GFF1073 0.55 -0.4 384,811 - GFF1073 0.55 -0.3 384,849 + GFF1073 0.61 +2.1 384,849 + GFF1073 0.61 +0.7 384,849 + GFF1073 0.61 -1.5 384,849 + GFF1073 0.61 -0.3 384,849 + GFF1073 0.61 -2.3 384,849 + GFF1073 0.61 -0.2 384,849 + GFF1073 0.61 +0.6 384,849 + GFF1073 0.61 -0.7 384,849 + GFF1073 0.61 -0.5 384,849 + GFF1073 0.61 -0.1 384,850 - GFF1073 0.61 +0.5 384,850 - GFF1073 0.61 +0.1 384,850 - GFF1073 0.61 -0.9 384,850 - GFF1073 0.61 -3.4 384,850 - GFF1073 0.61 +0.0 384,850 - GFF1073 0.61 +0.8 384,903 + GFF1073 0.69 -1.1 384,903 + GFF1073 0.69 -0.9 384,903 + GFF1073 0.69 -0.6 384,903 + GFF1073 0.69 -0.6 384,903 + GFF1073 0.69 +0.5 384,904 - GFF1073 0.69 -1.0 385,111 - +0.8 385,161 + -0.6 385,161 + -0.1
Or see this region's nucleotide sequence