Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1061 and GFF1062 overlap by 1 nucleotides GFF1062 and GFF1063 are separated by 41 nucleotides GFF1063 and GFF1064 are separated by 75 nucleotides GFF1064 and GFF1065 are separated by 59 nucleotides
GFF1061 - Hydroxymethylpyrimidine ABC transporter, transmembrane component, at 374,928 to 375,686
GFF1061
GFF1062 - Ribulose-5-phosphate 4-epimerase and related epimerases and aldolases, at 375,686 to 376,462
GFF1062
GFF1063 - dioxygenase, TauD/TfdA family, at 376,504 to 377,343
GFF1063
GFF1064 - Transcriptional regulator, GntR family, at 377,419 to 378,153
GFF1064
GFF1065 - Oxidoreductase, short-chain dehydrogenase/reductase family, at 378,213 to 379,046
GFF1065
Position (kb)
376
377
378 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 375.561 kb on + strand, within GFF1061 at 375.562 kb on - strand, within GFF1061 at 375.562 kb on - strand, within GFF1061 at 375.562 kb on - strand, within GFF1061 at 375.562 kb on - strand, within GFF1061 at 375.562 kb on - strand, within GFF1061 at 375.761 kb on + strand at 375.800 kb on + strand, within GFF1062 at 375.800 kb on + strand, within GFF1062 at 375.801 kb on - strand, within GFF1062 at 375.801 kb on - strand, within GFF1062 at 375.971 kb on - strand, within GFF1062 at 376.118 kb on + strand, within GFF1062 at 376.253 kb on + strand, within GFF1062 at 376.253 kb on + strand, within GFF1062 at 376.253 kb on + strand, within GFF1062 at 376.253 kb on + strand, within GFF1062 at 376.254 kb on - strand, within GFF1062 at 376.256 kb on + strand, within GFF1062 at 376.256 kb on + strand, within GFF1062 at 376.257 kb on - strand, within GFF1062 at 376.257 kb on - strand, within GFF1062 at 376.326 kb on - strand, within GFF1062 at 376.729 kb on + strand, within GFF1063 at 376.741 kb on + strand, within GFF1063 at 376.741 kb on + strand, within GFF1063 at 376.822 kb on + strand, within GFF1063 at 376.823 kb on - strand, within GFF1063 at 376.823 kb on - strand, within GFF1063 at 376.823 kb on - strand, within GFF1063 at 376.976 kb on - strand, within GFF1063 at 376.976 kb on - strand, within GFF1063 at 376.976 kb on - strand, within GFF1063 at 376.984 kb on + strand, within GFF1063 at 376.985 kb on - strand, within GFF1063 at 376.988 kb on - strand, within GFF1063 at 377.101 kb on + strand, within GFF1063 at 377.101 kb on + strand, within GFF1063 at 377.102 kb on - strand, within GFF1063 at 377.102 kb on - strand, within GFF1063 at 377.206 kb on + strand, within GFF1063 at 377.861 kb on - strand, within GFF1064 at 377.893 kb on + strand, within GFF1064 at 377.893 kb on + strand, within GFF1064 at 377.894 kb on - strand, within GFF1064 at 377.894 kb on - strand, within GFF1064 at 377.914 kb on + strand, within GFF1064 at 377.914 kb on + strand, within GFF1064 at 377.915 kb on - strand, within GFF1064 at 377.915 kb on - strand, within GFF1064 at 377.915 kb on - strand, within GFF1064 at 377.959 kb on + strand, within GFF1064 at 377.960 kb on - strand, within GFF1064 at 377.968 kb on + strand, within GFF1064 at 377.968 kb on + strand, within GFF1064 at 378.242 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 375,561 + GFF1061 0.83 +1.0 375,562 - GFF1061 0.84 +1.3 375,562 - GFF1061 0.84 +0.2 375,562 - GFF1061 0.84 -1.1 375,562 - GFF1061 0.84 +0.8 375,562 - GFF1061 0.84 +0.5 375,761 + -0.7 375,800 + GFF1062 0.15 -0.0 375,800 + GFF1062 0.15 -0.4 375,801 - GFF1062 0.15 -1.5 375,801 - GFF1062 0.15 +0.3 375,971 - GFF1062 0.37 -0.1 376,118 + GFF1062 0.56 +0.6 376,253 + GFF1062 0.73 +0.2 376,253 + GFF1062 0.73 -2.2 376,253 + GFF1062 0.73 +1.4 376,253 + GFF1062 0.73 +2.7 376,254 - GFF1062 0.73 -0.1 376,256 + GFF1062 0.73 +0.4 376,256 + GFF1062 0.73 -0.3 376,257 - GFF1062 0.73 +0.9 376,257 - GFF1062 0.73 +0.4 376,326 - GFF1062 0.82 -0.8 376,729 + GFF1063 0.27 -1.1 376,741 + GFF1063 0.28 -0.3 376,741 + GFF1063 0.28 +1.5 376,822 + GFF1063 0.38 -0.7 376,823 - GFF1063 0.38 -0.4 376,823 - GFF1063 0.38 +0.8 376,823 - GFF1063 0.38 -0.3 376,976 - GFF1063 0.56 -0.0 376,976 - GFF1063 0.56 +0.4 376,976 - GFF1063 0.56 -0.5 376,984 + GFF1063 0.57 -0.5 376,985 - GFF1063 0.57 +0.3 376,988 - GFF1063 0.58 -0.3 377,101 + GFF1063 0.71 -1.1 377,101 + GFF1063 0.71 -0.9 377,102 - GFF1063 0.71 -0.5 377,102 - GFF1063 0.71 +0.2 377,206 + GFF1063 0.84 -0.5 377,861 - GFF1064 0.60 -1.1 377,893 + GFF1064 0.64 -0.8 377,893 + GFF1064 0.64 +0.0 377,894 - GFF1064 0.65 -0.9 377,894 - GFF1064 0.65 +0.3 377,914 + GFF1064 0.67 +0.3 377,914 + GFF1064 0.67 +0.9 377,915 - GFF1064 0.67 +1.1 377,915 - GFF1064 0.67 +0.4 377,915 - GFF1064 0.67 -0.0 377,959 + GFF1064 0.73 +0.8 377,960 - GFF1064 0.74 -0.5 377,968 + GFF1064 0.75 +0.3 377,968 + GFF1064 0.75 -0.0 378,242 - +1.0
Or see this region's nucleotide sequence