Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF8 and GFF9 are separated by 19 nucleotides GFF9 and GFF10 overlap by 8 nucleotides
GFF8 - RND efflux system, outer membrane lipoprotein, NodT family, at 6,078 to 7,592
GFF8
GFF9 - CzcABC family efflux RND transporter, transmembrane protein, at 7,612 to 10,707
GFF9
GFF10 - Cobalt/zinc/cadmium efflux RND transporter, membrane fusion protein, CzcB family, at 10,700 to 11,854
GFF10
Position (kb)
7
8
9
10
11 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 6.934 kb on + strand, within GFF8 at 6.935 kb on - strand, within GFF8 at 7.139 kb on - strand, within GFF8 at 7.238 kb on - strand, within GFF8 at 7.477 kb on + strand at 7.477 kb on + strand at 7.477 kb on + strand at 7.478 kb on - strand at 7.478 kb on - strand at 7.478 kb on - strand at 7.478 kb on - strand at 7.478 kb on - strand at 7.510 kb on + strand at 7.510 kb on + strand at 7.510 kb on + strand at 7.511 kb on - strand at 7.934 kb on - strand, within GFF9 at 8.092 kb on + strand, within GFF9 at 8.093 kb on - strand, within GFF9 at 8.243 kb on + strand, within GFF9 at 8.244 kb on - strand, within GFF9 at 8.244 kb on - strand, within GFF9 at 8.453 kb on + strand, within GFF9 at 8.454 kb on - strand, within GFF9 at 8.454 kb on - strand, within GFF9 at 8.454 kb on - strand, within GFF9 at 8.471 kb on + strand, within GFF9 at 8.472 kb on - strand, within GFF9 at 8.472 kb on - strand, within GFF9 at 8.589 kb on + strand, within GFF9 at 8.693 kb on + strand, within GFF9 at 8.693 kb on + strand, within GFF9 at 8.694 kb on - strand, within GFF9 at 8.694 kb on - strand, within GFF9 at 8.770 kb on + strand, within GFF9 at 8.770 kb on + strand, within GFF9 at 8.771 kb on - strand, within GFF9 at 8.771 kb on - strand, within GFF9 at 8.771 kb on - strand, within GFF9 at 8.771 kb on - strand, within GFF9 at 8.771 kb on - strand, within GFF9 at 8.856 kb on - strand, within GFF9 at 8.942 kb on + strand, within GFF9 at 8.942 kb on + strand, within GFF9 at 8.942 kb on + strand, within GFF9 at 8.942 kb on + strand, within GFF9 at 8.942 kb on + strand, within GFF9 at 8.943 kb on - strand, within GFF9 at 8.943 kb on - strand, within GFF9 at 8.943 kb on - strand, within GFF9 at 9.134 kb on + strand, within GFF9 at 9.245 kb on + strand, within GFF9 at 9.246 kb on - strand, within GFF9 at 9.246 kb on - strand, within GFF9 at 9.246 kb on - strand, within GFF9 at 9.299 kb on + strand, within GFF9 at 9.299 kb on + strand, within GFF9 at 9.299 kb on + strand, within GFF9 at 9.300 kb on - strand, within GFF9 at 9.300 kb on - strand, within GFF9 at 9.368 kb on + strand, within GFF9 at 9.749 kb on + strand, within GFF9 at 9.750 kb on - strand, within GFF9 at 9.750 kb on - strand, within GFF9 at 9.750 kb on - strand, within GFF9 at 9.750 kb on - strand, within GFF9 at 9.750 kb on - strand, within GFF9 at 9.842 kb on + strand, within GFF9 at 9.843 kb on - strand, within GFF9 at 10.034 kb on + strand, within GFF9 at 10.034 kb on + strand, within GFF9 at 10.034 kb on + strand, within GFF9 at 10.034 kb on + strand, within GFF9 at 10.035 kb on - strand, within GFF9 at 10.035 kb on - strand, within GFF9 at 10.035 kb on - strand, within GFF9 at 10.035 kb on - strand, within GFF9 at 10.124 kb on + strand, within GFF9 at 10.124 kb on + strand, within GFF9 at 10.289 kb on + strand, within GFF9 at 10.290 kb on - strand, within GFF9 at 10.295 kb on + strand, within GFF9 at 10.295 kb on + strand, within GFF9 at 10.296 kb on - strand, within GFF9 at 10.296 kb on - strand, within GFF9 at 10.397 kb on + strand, within GFF9 at 10.397 kb on + strand, within GFF9 at 10.398 kb on - strand at 10.592 kb on + strand at 10.592 kb on + strand at 10.701 kb on + strand at 10.701 kb on + strand at 10.929 kb on + strand, within GFF10 at 10.929 kb on + strand, within GFF10 at 10.929 kb on + strand, within GFF10 at 10.929 kb on + strand, within GFF10 at 10.929 kb on + strand, within GFF10 at 10.929 kb on + strand, within GFF10 at 10.929 kb on + strand, within GFF10 at 10.929 kb on + strand, within GFF10 at 10.929 kb on + strand, within GFF10 at 10.930 kb on - strand, within GFF10 at 10.930 kb on - strand, within GFF10 at 10.930 kb on - strand, within GFF10 at 10.930 kb on - strand, within GFF10 at 10.930 kb on - strand, within GFF10 at 10.930 kb on - strand, within GFF10 at 10.930 kb on - strand, within GFF10 at 10.930 kb on - strand, within GFF10 at 10.930 kb on - strand, within GFF10 at 11.597 kb on + strand, within GFF10 at 11.597 kb on + strand, within GFF10 at 11.598 kb on - strand, within GFF10 at 11.598 kb on - strand, within GFF10
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 6,934 + GFF8 0.57 -1.1 6,935 - GFF8 0.57 -0.5 7,139 - GFF8 0.70 -1.1 7,238 - GFF8 0.77 -0.6 7,477 + -0.7 7,477 + -0.1 7,477 + -0.3 7,478 - -1.0 7,478 - +0.7 7,478 - -0.1 7,478 - -0.3 7,478 - -0.3 7,510 + +1.3 7,510 + -0.2 7,510 + -1.1 7,511 - +0.3 7,934 - GFF9 0.10 +0.5 8,092 + GFF9 0.16 -0.6 8,093 - GFF9 0.16 -0.9 8,243 + GFF9 0.20 -0.6 8,244 - GFF9 0.20 +0.8 8,244 - GFF9 0.20 +0.4 8,453 + GFF9 0.27 -0.6 8,454 - GFF9 0.27 -0.4 8,454 - GFF9 0.27 -1.2 8,454 - GFF9 0.27 +0.5 8,471 + GFF9 0.28 -0.0 8,472 - GFF9 0.28 +0.8 8,472 - GFF9 0.28 -0.9 8,589 + GFF9 0.32 -0.5 8,693 + GFF9 0.35 +0.0 8,693 + GFF9 0.35 -0.1 8,694 - GFF9 0.35 -0.8 8,694 - GFF9 0.35 -0.1 8,770 + GFF9 0.37 +0.0 8,770 + GFF9 0.37 +0.1 8,771 - GFF9 0.37 -0.5 8,771 - GFF9 0.37 -0.6 8,771 - GFF9 0.37 +0.8 8,771 - GFF9 0.37 -0.3 8,771 - GFF9 0.37 +0.6 8,856 - GFF9 0.40 -1.2 8,942 + GFF9 0.43 +0.5 8,942 + GFF9 0.43 -0.9 8,942 + GFF9 0.43 -1.5 8,942 + GFF9 0.43 -2.1 8,942 + GFF9 0.43 -0.6 8,943 - GFF9 0.43 -1.0 8,943 - GFF9 0.43 -0.7 8,943 - GFF9 0.43 -1.2 9,134 + GFF9 0.49 -0.5 9,245 + GFF9 0.53 -0.2 9,246 - GFF9 0.53 -0.1 9,246 - GFF9 0.53 -0.2 9,246 - GFF9 0.53 -0.0 9,299 + GFF9 0.54 +0.1 9,299 + GFF9 0.54 -0.3 9,299 + GFF9 0.54 -0.1 9,300 - GFF9 0.55 -0.1 9,300 - GFF9 0.55 +1.0 9,368 + GFF9 0.57 -0.5 9,749 + GFF9 0.69 -1.1 9,750 - GFF9 0.69 -0.7 9,750 - GFF9 0.69 -0.9 9,750 - GFF9 0.69 -0.0 9,750 - GFF9 0.69 +0.3 9,750 - GFF9 0.69 -0.6 9,842 + GFF9 0.72 -1.4 9,843 - GFF9 0.72 -0.2 10,034 + GFF9 0.78 -0.4 10,034 + GFF9 0.78 -0.1 10,034 + GFF9 0.78 -0.2 10,034 + GFF9 0.78 -0.8 10,035 - GFF9 0.78 -0.5 10,035 - GFF9 0.78 -0.4 10,035 - GFF9 0.78 -0.8 10,035 - GFF9 0.78 +2.6 10,124 + GFF9 0.81 +0.3 10,124 + GFF9 0.81 -0.5 10,289 + GFF9 0.86 +1.5 10,290 - GFF9 0.86 -0.8 10,295 + GFF9 0.87 -1.0 10,295 + GFF9 0.87 -1.1 10,296 - GFF9 0.87 -0.1 10,296 - GFF9 0.87 -0.0 10,397 + GFF9 0.90 -0.4 10,397 + GFF9 0.90 -1.9 10,398 - -0.4 10,592 + +0.2 10,592 + +0.1 10,701 + -1.1 10,701 + +0.2 10,929 + GFF10 0.20 -0.2 10,929 + GFF10 0.20 +0.1 10,929 + GFF10 0.20 +0.1 10,929 + GFF10 0.20 -1.4 10,929 + GFF10 0.20 -0.3 10,929 + GFF10 0.20 -0.4 10,929 + GFF10 0.20 +0.6 10,929 + GFF10 0.20 -0.4 10,929 + GFF10 0.20 +0.3 10,930 - GFF10 0.20 -0.0 10,930 - GFF10 0.20 +0.6 10,930 - GFF10 0.20 +0.1 10,930 - GFF10 0.20 -1.1 10,930 - GFF10 0.20 +0.3 10,930 - GFF10 0.20 +0.3 10,930 - GFF10 0.20 +0.3 10,930 - GFF10 0.20 -0.3 10,930 - GFF10 0.20 -0.9 11,597 + GFF10 0.78 -0.5 11,597 + GFF10 0.78 -0.3 11,598 - GFF10 0.78 -0.9 11,598 - GFF10 0.78 -0.7
Or see this region's nucleotide sequence