Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6953 and GFF6954 are separated by 183 nucleotides GFF6954 and GFF6955 are separated by 7 nucleotides GFF6955 and GFF6956 are separated by 260 nucleotides
GFF6953 - Universal stress protein UspA and related nucleotide-binding proteins, at 29,511 to 30,335
GFF6953
GFF6954 - no description, at 30,519 to 30,722
GFF6954
GFF6955 - no description, at 30,730 to 30,864
GFF6955
GFF6956 - Heavy-metal-associated domain (N-terminus) and membrane-bounded cytochrome biogenesis cycZ-like domain, possible membrane copper tolerance protein, at 31,125 to 31,820
GFF6956
Position (kb) 30
31 Strain fitness (log2 ratio) -1
0
1
2 at 29.728 kb on + strand, within GFF6953 at 29.729 kb on - strand, within GFF6953 at 29.729 kb on - strand, within GFF6953 at 29.899 kb on + strand, within GFF6953 at 30.019 kb on + strand, within GFF6953 at 30.019 kb on + strand, within GFF6953 at 30.019 kb on + strand, within GFF6953 at 30.019 kb on + strand, within GFF6953 at 30.019 kb on + strand, within GFF6953 at 30.020 kb on - strand, within GFF6953 at 30.020 kb on - strand, within GFF6953 at 30.020 kb on - strand, within GFF6953 at 30.022 kb on - strand, within GFF6953 at 30.187 kb on + strand, within GFF6953 at 30.187 kb on + strand, within GFF6953 at 31.637 kb on - strand, within GFF6956
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 29,728 + GFF6953 0.26 +2.2 29,729 - GFF6953 0.26 +0.2 29,729 - GFF6953 0.26 -1.3 29,899 + GFF6953 0.47 -0.0 30,019 + GFF6953 0.62 -1.1 30,019 + GFF6953 0.62 -0.6 30,019 + GFF6953 0.62 -0.8 30,019 + GFF6953 0.62 +0.4 30,019 + GFF6953 0.62 -0.5 30,020 - GFF6953 0.62 -0.2 30,020 - GFF6953 0.62 -0.0 30,020 - GFF6953 0.62 -1.4 30,022 - GFF6953 0.62 -0.0 30,187 + GFF6953 0.82 -0.5 30,187 + GFF6953 0.82 -1.2 31,637 - GFF6956 0.74 -1.0
Or see this region's nucleotide sequence