Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6947 and GFF6948 are separated by 235 nucleotides GFF6948 and GFF6949 are separated by 136 nucleotides GFF6949 and GFF6950 are separated by 1 nucleotides GFF6950 and GFF6951 are separated by 99 nucleotides
GFF6947 - transcriptional regulator, Crp/Fnr family, at 24,023 to 24,790
GFF6947
GFF6948 - Ornithine carbamoyltransferase (EC 2.1.3.3), at 25,026 to 25,529
GFF6948
GFF6949 - no description, at 25,666 to 26,319
GFF6949
GFF6950 - Acetoacetyl-CoA reductase (EC 1.1.1.36), at 26,321 to 27,040
GFF6950
GFF6951 - Alcohol dehydrogenase (EC 1.1.1.1), at 27,140 to 28,129
GFF6951
Position (kb) 25
26
27 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 24.791 kb on - strand at 24.832 kb on + strand at 24.832 kb on + strand at 24.833 kb on - strand at 24.877 kb on + strand at 24.909 kb on + strand at 25.182 kb on + strand, within GFF6948 at 25.183 kb on - strand, within GFF6948 at 25.330 kb on - strand, within GFF6948 at 25.330 kb on - strand, within GFF6948 at 25.591 kb on + strand at 25.591 kb on + strand at 25.837 kb on + strand, within GFF6949 at 25.838 kb on - strand, within GFF6949 at 26.023 kb on + strand, within GFF6949 at 26.024 kb on - strand, within GFF6949 at 26.252 kb on + strand, within GFF6949 at 26.253 kb on - strand, within GFF6949 at 26.756 kb on + strand, within GFF6950 at 26.757 kb on - strand, within GFF6950 at 26.757 kb on - strand, within GFF6950 at 26.852 kb on + strand, within GFF6950 at 26.853 kb on - strand, within GFF6950 at 27.029 kb on + strand at 27.029 kb on + strand at 27.029 kb on + strand at 27.029 kb on + strand at 27.029 kb on + strand at 27.030 kb on - strand at 27.030 kb on - strand at 27.030 kb on - strand at 27.030 kb on - strand at 27.082 kb on + strand at 27.168 kb on + strand at 27.168 kb on + strand at 27.169 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 24,791 - +0.6 24,832 + -0.5 24,832 + +0.0 24,833 - +2.0 24,877 + +0.4 24,909 + -0.5 25,182 + GFF6948 0.31 -1.8 25,183 - GFF6948 0.31 +3.2 25,330 - GFF6948 0.60 -0.5 25,330 - GFF6948 0.60 -0.4 25,591 + +0.2 25,591 + -0.0 25,837 + GFF6949 0.26 -0.8 25,838 - GFF6949 0.26 +0.8 26,023 + GFF6949 0.55 +1.0 26,024 - GFF6949 0.55 -1.3 26,252 + GFF6949 0.90 +1.3 26,253 - GFF6949 0.90 +0.3 26,756 + GFF6950 0.60 -2.4 26,757 - GFF6950 0.61 +0.4 26,757 - GFF6950 0.61 -1.4 26,852 + GFF6950 0.74 -0.2 26,853 - GFF6950 0.74 -0.4 27,029 + -0.6 27,029 + -0.1 27,029 + -0.7 27,029 + -0.4 27,029 + +0.2 27,030 - -3.4 27,030 - -0.3 27,030 - -0.7 27,030 - -1.2 27,082 + -0.4 27,168 + -0.1 27,168 + -0.8 27,169 - -3.1
Or see this region's nucleotide sequence