Strain Fitness in Variovorax sp. SCN45 around GFF6701

Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF6700 and GFF6701 overlap by 4 nucleotidesGFF6701 and GFF6702 are separated by 55 nucleotides GFF6700 - Single-stranded-DNA-specific exonuclease RecJ, at 8,257 to 9,984 GFF6700 GFF6701 - Regulatory protein, RpfE type, at 9,981 to 10,949 GFF6701 GFF6702 - Lipoprotein releasing system transmembrane protein LolC/LolE, at 11,005 to 12,261 GFF6702 Position (kb) 9 10 11Strain fitness (log2 ratio) -1 0 1 2 3 4at 9.398 kb on + strand, within GFF6700at 9.398 kb on + strand, within GFF6700at 9.398 kb on + strand, within GFF6700at 9.398 kb on + strand, within GFF6700at 9.399 kb on - strand, within GFF6700at 9.399 kb on - strand, within GFF6700at 9.674 kb on + strand, within GFF6700at 9.674 kb on + strand, within GFF6700at 9.675 kb on - strand, within GFF6700at 9.750 kb on - strand, within GFF6700at 9.985 kb on - strandat 10.066 kb on + strandat 10.066 kb on + strandat 10.067 kb on - strandat 10.067 kb on - strandat 10.123 kb on + strand, within GFF6701at 10.351 kb on + strand, within GFF6701at 10.352 kb on - strand, within GFF6701at 10.352 kb on - strand, within GFF6701at 10.955 kb on + strandat 10.956 kb on - strandat 11.004 kb on + strandat 11.004 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
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9,398 + GFF6700 0.66 -0.6
9,398 + GFF6700 0.66 -0.7
9,398 + GFF6700 0.66 -1.6
9,398 + GFF6700 0.66 +0.0
9,399 - GFF6700 0.66 +1.1
9,399 - GFF6700 0.66 -0.2
9,674 + GFF6700 0.82 +1.3
9,674 + GFF6700 0.82 +0.2
9,675 - GFF6700 0.82 -0.4
9,750 - GFF6700 0.86 -0.8
9,985 - -0.1
10,066 + +1.6
10,066 + -0.5
10,067 - +1.2
10,067 - -1.0
10,123 + GFF6701 0.15 +0.6
10,351 + GFF6701 0.38 -1.4
10,352 - GFF6701 0.38 -0.7
10,352 - GFF6701 0.38 -1.1
10,955 + +3.9
10,956 - +0.2
11,004 + -1.2
11,004 + -0.3

Or see this region's nucleotide sequence