Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6428 and GFF6429 overlap by 4 nucleotides GFF6429 and GFF6430 overlap by 4 nucleotides GFF6430 and GFF6431 are separated by 112 nucleotides GFF6431 and GFF6432 overlap by 4 nucleotides
GFF6428 - Glyoxalase/bleomycin resistance protein/dioxygenase, at 1,988 to 2,368
GFF6428
GFF6429 - Pyruvate:Oxaloacetate transcarboxylase domain protein, at 2,365 to 3,348
GFF6429
GFF6430 - L-carnitine dehydratase/bile acid-inducible protein F, at 3,345 to 4,517
GFF6430
GFF6431 - hypothetical protein, at 4,630 to 5,406
GFF6431
GFF6432 - no description, at 5,403 to 6,131
GFF6432
Position (kb)
3
4
5 Strain fitness (log2 ratio)
-2
-1
0
1 at 2.372 kb on + strand at 2.372 kb on + strand at 2.793 kb on - strand, within GFF6429 at 3.113 kb on + strand, within GFF6429 at 3.218 kb on + strand, within GFF6429 at 3.536 kb on - strand, within GFF6430 at 3.536 kb on - strand, within GFF6430 at 3.568 kb on + strand, within GFF6430 at 3.568 kb on + strand, within GFF6430 at 3.568 kb on + strand, within GFF6430 at 3.569 kb on - strand, within GFF6430 at 3.569 kb on - strand, within GFF6430 at 3.569 kb on - strand, within GFF6430 at 3.569 kb on - strand, within GFF6430 at 3.569 kb on - strand, within GFF6430 at 3.569 kb on - strand, within GFF6430 at 3.569 kb on - strand, within GFF6430 at 3.569 kb on - strand, within GFF6430 at 3.569 kb on - strand, within GFF6430 at 3.570 kb on + strand, within GFF6430 at 3.571 kb on - strand, within GFF6430 at 3.787 kb on + strand, within GFF6430 at 3.787 kb on + strand, within GFF6430 at 3.787 kb on + strand, within GFF6430 at 3.787 kb on + strand, within GFF6430 at 3.787 kb on + strand, within GFF6430 at 3.787 kb on + strand, within GFF6430 at 3.788 kb on - strand, within GFF6430 at 3.788 kb on - strand, within GFF6430 at 3.788 kb on - strand, within GFF6430 at 3.788 kb on - strand, within GFF6430 at 3.788 kb on - strand, within GFF6430 at 3.788 kb on - strand, within GFF6430 at 3.809 kb on - strand, within GFF6430 at 3.919 kb on + strand, within GFF6430 at 4.072 kb on + strand, within GFF6430 at 4.073 kb on - strand, within GFF6430 at 4.541 kb on + strand at 4.542 kb on - strand at 4.542 kb on - strand at 4.554 kb on + strand at 4.631 kb on + strand at 4.748 kb on + strand, within GFF6431 at 4.749 kb on - strand, within GFF6431 at 4.749 kb on - strand, within GFF6431 at 4.749 kb on - strand, within GFF6431 at 4.916 kb on + strand, within GFF6431 at 5.159 kb on + strand, within GFF6431 at 5.336 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 2,372 + +1.4 2,372 + -1.2 2,793 - GFF6429 0.43 -0.0 3,113 + GFF6429 0.76 -0.4 3,218 + GFF6429 0.87 +0.4 3,536 - GFF6430 0.16 -0.4 3,536 - GFF6430 0.16 -0.4 3,568 + GFF6430 0.19 -0.2 3,568 + GFF6430 0.19 -0.2 3,568 + GFF6430 0.19 +0.2 3,569 - GFF6430 0.19 +0.0 3,569 - GFF6430 0.19 -0.8 3,569 - GFF6430 0.19 +0.4 3,569 - GFF6430 0.19 +0.2 3,569 - GFF6430 0.19 -0.7 3,569 - GFF6430 0.19 -2.4 3,569 - GFF6430 0.19 -0.1 3,569 - GFF6430 0.19 -0.3 3,569 - GFF6430 0.19 -0.4 3,570 + GFF6430 0.19 -0.5 3,571 - GFF6430 0.19 -0.9 3,787 + GFF6430 0.38 -0.9 3,787 + GFF6430 0.38 -0.1 3,787 + GFF6430 0.38 -1.2 3,787 + GFF6430 0.38 -1.0 3,787 + GFF6430 0.38 -1.0 3,787 + GFF6430 0.38 -0.5 3,788 - GFF6430 0.38 -0.8 3,788 - GFF6430 0.38 -0.1 3,788 - GFF6430 0.38 -0.2 3,788 - GFF6430 0.38 -0.6 3,788 - GFF6430 0.38 -0.2 3,788 - GFF6430 0.38 +0.8 3,809 - GFF6430 0.40 +0.1 3,919 + GFF6430 0.49 +0.4 4,072 + GFF6430 0.62 -0.3 4,073 - GFF6430 0.62 -0.3 4,541 + +0.8 4,542 - -0.4 4,542 - +0.6 4,554 + -0.6 4,631 + -1.1 4,748 + GFF6431 0.15 +0.1 4,749 - GFF6431 0.15 +0.4 4,749 - GFF6431 0.15 +0.8 4,749 - GFF6431 0.15 +1.4 4,916 + GFF6431 0.37 +0.4 5,159 + GFF6431 0.68 -1.1 5,336 + -0.1
Or see this region's nucleotide sequence