Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6016 and GFF6017 are separated by 125 nucleotides GFF6017 and GFF6018 overlap by 4 nucleotides GFF6018 and GFF6019 are separated by 7 nucleotides GFF6019 and GFF6020 overlap by 1 nucleotides
GFF6016 - Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases), at 76,215 to 77,144
GFF6016
GFF6017 - NADPH-dependent 7-cyano-7-deazaguanine reductase (EC 1.7.1.13), at 77,270 to 78,136
GFF6017
GFF6018 - Hydrolase, alpha/beta fold family functionally coupled to Phosphoribulokinase, at 78,133 to 79,035
GFF6018
GFF6019 - Similar to eukaryotic Peptidyl prolyl 4-hydroxylase, alpha subunit (EC 1.14.11.2), at 79,043 to 79,885
GFF6019
GFF6020 - UPF0246 protein YaaA, at 79,885 to 80,667
GFF6020
Position (kb)
78
79
80 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 77.358 kb on + strand, within GFF6017 at 77.359 kb on - strand, within GFF6017 at 77.359 kb on - strand, within GFF6017 at 77.367 kb on + strand, within GFF6017 at 77.367 kb on + strand, within GFF6017 at 77.368 kb on - strand, within GFF6017 at 77.368 kb on - strand, within GFF6017 at 77.469 kb on + strand, within GFF6017 at 77.469 kb on + strand, within GFF6017 at 77.469 kb on + strand, within GFF6017 at 77.470 kb on - strand, within GFF6017 at 77.470 kb on - strand, within GFF6017 at 77.470 kb on - strand, within GFF6017 at 77.508 kb on + strand, within GFF6017 at 77.640 kb on + strand, within GFF6017 at 77.851 kb on - strand, within GFF6017 at 78.063 kb on + strand at 78.076 kb on - strand at 78.416 kb on + strand, within GFF6018 at 78.417 kb on - strand, within GFF6018 at 78.417 kb on - strand, within GFF6018 at 79.044 kb on + strand at 79.044 kb on + strand at 79.149 kb on + strand, within GFF6019 at 79.149 kb on + strand, within GFF6019 at 79.150 kb on - strand, within GFF6019 at 79.242 kb on + strand, within GFF6019 at 79.242 kb on + strand, within GFF6019 at 79.242 kb on + strand, within GFF6019 at 79.242 kb on + strand, within GFF6019 at 79.242 kb on + strand, within GFF6019 at 79.243 kb on - strand, within GFF6019 at 79.243 kb on - strand, within GFF6019 at 79.243 kb on - strand, within GFF6019 at 79.243 kb on - strand, within GFF6019 at 79.243 kb on - strand, within GFF6019 at 79.318 kb on - strand, within GFF6019 at 79.318 kb on - strand, within GFF6019 at 79.318 kb on - strand, within GFF6019 at 79.324 kb on - strand, within GFF6019 at 79.660 kb on - strand, within GFF6019 at 79.943 kb on + strand at 79.996 kb on + strand, within GFF6020
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 77,358 + GFF6017 0.10 +1.8 77,359 - GFF6017 0.10 -0.6 77,359 - GFF6017 0.10 -0.3 77,367 + GFF6017 0.11 +0.1 77,367 + GFF6017 0.11 +0.6 77,368 - GFF6017 0.11 -0.4 77,368 - GFF6017 0.11 -2.4 77,469 + GFF6017 0.23 -1.6 77,469 + GFF6017 0.23 -0.3 77,469 + GFF6017 0.23 -0.1 77,470 - GFF6017 0.23 -2.7 77,470 - GFF6017 0.23 -0.4 77,470 - GFF6017 0.23 +0.7 77,508 + GFF6017 0.27 +1.0 77,640 + GFF6017 0.43 -0.6 77,851 - GFF6017 0.67 -1.0 78,063 + -0.5 78,076 - -0.9 78,416 + GFF6018 0.31 -0.8 78,417 - GFF6018 0.31 +0.9 78,417 - GFF6018 0.31 +0.2 79,044 + +0.6 79,044 + -2.2 79,149 + GFF6019 0.13 -0.0 79,149 + GFF6019 0.13 -0.4 79,150 - GFF6019 0.13 -0.4 79,242 + GFF6019 0.24 -0.5 79,242 + GFF6019 0.24 -1.5 79,242 + GFF6019 0.24 -0.0 79,242 + GFF6019 0.24 -0.9 79,242 + GFF6019 0.24 +0.4 79,243 - GFF6019 0.24 -0.1 79,243 - GFF6019 0.24 +0.1 79,243 - GFF6019 0.24 -2.0 79,243 - GFF6019 0.24 +0.7 79,243 - GFF6019 0.24 -0.1 79,318 - GFF6019 0.33 -0.4 79,318 - GFF6019 0.33 -0.5 79,318 - GFF6019 0.33 -0.3 79,324 - GFF6019 0.33 -0.4 79,660 - GFF6019 0.73 -0.0 79,943 + -0.7 79,996 + GFF6020 0.14 -0.0
Or see this region's nucleotide sequence