Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5945 and GFF5946 are separated by 60 nucleotides
GFF5945 - Membrane-bound lytic murein transglycosylase A, at 166,195 to 167,796
GFF5945
GFF5946 - T6SS Serine/threonine protein kinase (EC 2.7.11.1) PpkA, at 167,857 to 169,785
GFF5946
Position (kb)
167
168
169
170 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 166.892 kb on + strand, within GFF5945 at 167.036 kb on + strand, within GFF5945 at 167.037 kb on - strand, within GFF5945 at 167.037 kb on - strand, within GFF5945 at 167.039 kb on + strand, within GFF5945 at 167.039 kb on + strand, within GFF5945 at 167.265 kb on - strand, within GFF5945 at 167.265 kb on - strand, within GFF5945 at 167.265 kb on - strand, within GFF5945 at 167.325 kb on - strand, within GFF5945 at 167.372 kb on + strand, within GFF5945 at 167.372 kb on + strand, within GFF5945 at 167.570 kb on + strand, within GFF5945 at 167.570 kb on + strand, within GFF5945 at 167.571 kb on - strand, within GFF5945 at 167.571 kb on - strand, within GFF5945 at 167.595 kb on + strand, within GFF5945 at 167.595 kb on + strand, within GFF5945 at 167.596 kb on - strand, within GFF5945 at 167.858 kb on + strand at 167.858 kb on + strand at 167.859 kb on - strand at 167.859 kb on - strand at 167.859 kb on - strand at 167.891 kb on + strand at 167.892 kb on - strand at 167.892 kb on - strand at 168.162 kb on - strand, within GFF5946 at 168.162 kb on - strand, within GFF5946 at 168.162 kb on - strand, within GFF5946 at 168.162 kb on - strand, within GFF5946 at 168.162 kb on - strand, within GFF5946 at 168.224 kb on + strand, within GFF5946 at 168.224 kb on + strand, within GFF5946 at 168.225 kb on - strand, within GFF5946 at 168.225 kb on - strand, within GFF5946 at 168.914 kb on + strand, within GFF5946 at 168.914 kb on + strand, within GFF5946 at 168.914 kb on + strand, within GFF5946 at 168.914 kb on + strand, within GFF5946 at 168.914 kb on + strand, within GFF5946 at 168.914 kb on + strand, within GFF5946 at 168.914 kb on + strand, within GFF5946 at 168.914 kb on + strand, within GFF5946 at 168.914 kb on + strand, within GFF5946 at 168.915 kb on - strand, within GFF5946 at 168.915 kb on - strand, within GFF5946 at 168.915 kb on - strand, within GFF5946 at 168.915 kb on - strand, within GFF5946 at 168.915 kb on - strand, within GFF5946 at 168.996 kb on - strand, within GFF5946 at 169.040 kb on + strand, within GFF5946 at 169.040 kb on + strand, within GFF5946 at 169.193 kb on + strand, within GFF5946 at 169.194 kb on - strand, within GFF5946 at 169.194 kb on - strand, within GFF5946 at 169.316 kb on + strand, within GFF5946 at 169.316 kb on + strand, within GFF5946 at 169.317 kb on - strand, within GFF5946 at 169.317 kb on - strand, within GFF5946 at 169.334 kb on + strand, within GFF5946 at 169.334 kb on + strand, within GFF5946 at 169.334 kb on + strand, within GFF5946 at 169.334 kb on + strand, within GFF5946 at 169.334 kb on + strand, within GFF5946 at 169.334 kb on + strand, within GFF5946 at 169.334 kb on + strand, within GFF5946 at 169.334 kb on + strand, within GFF5946 at 169.335 kb on - strand, within GFF5946 at 169.335 kb on - strand, within GFF5946 at 169.335 kb on - strand, within GFF5946 at 169.335 kb on - strand, within GFF5946 at 169.335 kb on - strand, within GFF5946 at 169.335 kb on - strand, within GFF5946 at 169.365 kb on - strand, within GFF5946 at 169.517 kb on + strand, within GFF5946 at 169.517 kb on + strand, within GFF5946 at 169.518 kb on - strand, within GFF5946 at 169.518 kb on - strand, within GFF5946 at 169.518 kb on - strand, within GFF5946 at 169.565 kb on + strand, within GFF5946 at 169.566 kb on - strand, within GFF5946 at 169.591 kb on + strand, within GFF5946 at 169.631 kb on - strand at 169.753 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 166,892 + GFF5945 0.44 -1.4 167,036 + GFF5945 0.52 -1.7 167,037 - GFF5945 0.53 -0.2 167,037 - GFF5945 0.53 +0.5 167,039 + GFF5945 0.53 -0.3 167,039 + GFF5945 0.53 -0.4 167,265 - GFF5945 0.67 +0.1 167,265 - GFF5945 0.67 -0.1 167,265 - GFF5945 0.67 +0.8 167,325 - GFF5945 0.71 +0.4 167,372 + GFF5945 0.73 +0.1 167,372 + GFF5945 0.73 +0.1 167,570 + GFF5945 0.86 -0.1 167,570 + GFF5945 0.86 -0.3 167,571 - GFF5945 0.86 +0.3 167,571 - GFF5945 0.86 +0.4 167,595 + GFF5945 0.87 +0.1 167,595 + GFF5945 0.87 -2.5 167,596 - GFF5945 0.87 -0.4 167,858 + +0.2 167,858 + -0.2 167,859 - +2.3 167,859 - +0.2 167,859 - -1.6 167,891 + -0.9 167,892 - -0.9 167,892 - -0.3 168,162 - GFF5946 0.16 -0.9 168,162 - GFF5946 0.16 -0.6 168,162 - GFF5946 0.16 -0.1 168,162 - GFF5946 0.16 -0.1 168,162 - GFF5946 0.16 +1.0 168,224 + GFF5946 0.19 +1.3 168,224 + GFF5946 0.19 -0.6 168,225 - GFF5946 0.19 -0.6 168,225 - GFF5946 0.19 -0.6 168,914 + GFF5946 0.55 +1.3 168,914 + GFF5946 0.55 -0.5 168,914 + GFF5946 0.55 +0.1 168,914 + GFF5946 0.55 +0.1 168,914 + GFF5946 0.55 +0.9 168,914 + GFF5946 0.55 +0.2 168,914 + GFF5946 0.55 -0.3 168,914 + GFF5946 0.55 -1.8 168,914 + GFF5946 0.55 -0.6 168,915 - GFF5946 0.55 -0.3 168,915 - GFF5946 0.55 +1.7 168,915 - GFF5946 0.55 -0.9 168,915 - GFF5946 0.55 +0.4 168,915 - GFF5946 0.55 -0.3 168,996 - GFF5946 0.59 +0.6 169,040 + GFF5946 0.61 +0.3 169,040 + GFF5946 0.61 -1.1 169,193 + GFF5946 0.69 -0.3 169,194 - GFF5946 0.69 -0.6 169,194 - GFF5946 0.69 +0.4 169,316 + GFF5946 0.76 +0.7 169,316 + GFF5946 0.76 +0.7 169,317 - GFF5946 0.76 -0.9 169,317 - GFF5946 0.76 +0.5 169,334 + GFF5946 0.77 +0.5 169,334 + GFF5946 0.77 +0.6 169,334 + GFF5946 0.77 +0.9 169,334 + GFF5946 0.77 -0.0 169,334 + GFF5946 0.77 -1.3 169,334 + GFF5946 0.77 +0.8 169,334 + GFF5946 0.77 -1.2 169,334 + GFF5946 0.77 +0.4 169,335 - GFF5946 0.77 +0.3 169,335 - GFF5946 0.77 -0.3 169,335 - GFF5946 0.77 +0.0 169,335 - GFF5946 0.77 +1.3 169,335 - GFF5946 0.77 +0.4 169,335 - GFF5946 0.77 -0.1 169,365 - GFF5946 0.78 -0.3 169,517 + GFF5946 0.86 +1.2 169,517 + GFF5946 0.86 -1.0 169,518 - GFF5946 0.86 +0.7 169,518 - GFF5946 0.86 -0.5 169,518 - GFF5946 0.86 -1.0 169,565 + GFF5946 0.89 -0.0 169,566 - GFF5946 0.89 +1.7 169,591 + GFF5946 0.90 -0.0 169,631 - +1.4 169,753 + +0.4
Or see this region's nucleotide sequence