Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF574 and GFF575 overlap by 4 nucleotides GFF575 and GFF576 are separated by 15 nucleotides GFF576 and GFF577 are separated by 3 nucleotides
GFF574 - Tol-Pal system-associated acyl-CoA thioesterase, at 619,742 to 620,200
GFF574
GFF575 - Tol-Pal system protein TolQ, at 620,197 to 620,919
GFF575
GFF576 - Tol biopolymer transport system, TolR protein, at 620,935 to 621,372
GFF576
GFF577 - TolA protein, at 621,376 to 622,299
GFF577
Position (kb) 620
621
622 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 619.969 kb on + strand, within GFF574 at 619.970 kb on - strand, within GFF574 at 620.008 kb on - strand, within GFF574 at 620.026 kb on - strand, within GFF574 at 620.134 kb on - strand, within GFF574 at 620.165 kb on + strand at 620.165 kb on + strand at 620.165 kb on + strand at 620.166 kb on - strand at 620.166 kb on - strand at 620.166 kb on - strand at 620.166 kb on - strand at 620.188 kb on + strand at 620.188 kb on + strand at 620.188 kb on + strand at 620.188 kb on + strand at 620.188 kb on + strand at 620.188 kb on + strand at 620.188 kb on + strand at 620.188 kb on + strand at 620.188 kb on + strand at 620.188 kb on + strand at 620.188 kb on + strand at 620.188 kb on + strand at 620.188 kb on + strand at 620.188 kb on + strand at 620.188 kb on + strand at 620.188 kb on + strand at 620.188 kb on + strand at 620.188 kb on + strand at 620.188 kb on + strand at 620.189 kb on - strand at 620.189 kb on - strand at 620.189 kb on - strand at 620.189 kb on - strand at 620.189 kb on - strand at 620.189 kb on - strand at 620.189 kb on - strand at 620.189 kb on - strand at 620.189 kb on - strand at 620.189 kb on - strand at 620.189 kb on - strand at 620.189 kb on - strand at 620.189 kb on - strand at 620.210 kb on + strand at 620.210 kb on + strand at 620.210 kb on + strand at 620.210 kb on + strand at 620.210 kb on + strand at 620.210 kb on + strand at 620.210 kb on + strand at 620.210 kb on + strand at 620.210 kb on + strand at 620.210 kb on + strand at 620.211 kb on - strand at 620.211 kb on - strand at 620.211 kb on - strand at 620.211 kb on - strand at 620.211 kb on - strand at 620.211 kb on - strand at 620.211 kb on - strand at 620.211 kb on - strand at 620.211 kb on - strand at 620.211 kb on - strand at 620.211 kb on - strand at 620.211 kb on - strand at 620.211 kb on - strand at 620.211 kb on - strand at 620.314 kb on + strand, within GFF575 at 620.314 kb on + strand, within GFF575 at 620.314 kb on + strand, within GFF575 at 620.314 kb on + strand, within GFF575 at 620.315 kb on - strand, within GFF575 at 620.315 kb on - strand, within GFF575 at 620.468 kb on - strand, within GFF575 at 620.468 kb on - strand, within GFF575 at 620.614 kb on + strand, within GFF575 at 620.614 kb on + strand, within GFF575 at 620.614 kb on + strand, within GFF575 at 620.614 kb on + strand, within GFF575 at 620.614 kb on + strand, within GFF575 at 620.614 kb on + strand, within GFF575 at 620.614 kb on + strand, within GFF575 at 620.614 kb on + strand, within GFF575 at 620.614 kb on + strand, within GFF575 at 620.614 kb on + strand, within GFF575 at 620.614 kb on + strand, within GFF575 at 620.614 kb on + strand, within GFF575 at 620.615 kb on - strand, within GFF575 at 620.615 kb on - strand, within GFF575 at 620.615 kb on - strand, within GFF575 at 620.615 kb on - strand, within GFF575 at 620.615 kb on - strand, within GFF575 at 620.615 kb on - strand, within GFF575 at 620.615 kb on - strand, within GFF575 at 620.615 kb on - strand, within GFF575 at 620.615 kb on - strand, within GFF575 at 620.615 kb on - strand, within GFF575 at 620.615 kb on - strand, within GFF575 at 620.615 kb on - strand, within GFF575 at 620.615 kb on - strand, within GFF575 at 620.615 kb on - strand, within GFF575 at 620.615 kb on - strand, within GFF575 at 620.615 kb on - strand, within GFF575 at 620.615 kb on - strand, within GFF575 at 620.615 kb on - strand, within GFF575 at 620.615 kb on - strand, within GFF575 at 620.773 kb on + strand, within GFF575 at 620.773 kb on + strand, within GFF575 at 620.774 kb on - strand, within GFF575 at 620.774 kb on - strand, within GFF575 at 620.774 kb on - strand, within GFF575 at 620.824 kb on + strand, within GFF575 at 620.824 kb on + strand, within GFF575 at 620.824 kb on + strand, within GFF575 at 620.824 kb on + strand, within GFF575 at 620.824 kb on + strand, within GFF575 at 620.824 kb on + strand, within GFF575 at 620.824 kb on + strand, within GFF575 at 620.824 kb on + strand, within GFF575 at 620.825 kb on - strand, within GFF575 at 620.825 kb on - strand, within GFF575 at 620.825 kb on - strand, within GFF575 at 620.825 kb on - strand, within GFF575 at 620.825 kb on - strand, within GFF575 at 620.825 kb on - strand, within GFF575 at 620.825 kb on - strand, within GFF575 at 620.825 kb on - strand, within GFF575 at 621.283 kb on + strand, within GFF576 at 621.283 kb on + strand, within GFF576 at 621.283 kb on + strand, within GFF576 at 621.283 kb on + strand, within GFF576 at 621.283 kb on + strand, within GFF576 at 621.284 kb on - strand, within GFF576 at 621.284 kb on - strand, within GFF576 at 621.284 kb on - strand, within GFF576 at 621.370 kb on + strand at 621.370 kb on + strand at 621.370 kb on + strand at 621.370 kb on + strand at 621.370 kb on + strand at 621.370 kb on + strand at 621.370 kb on + strand at 621.370 kb on + strand at 621.370 kb on + strand at 621.371 kb on - strand at 621.371 kb on - strand at 621.371 kb on - strand at 621.371 kb on - strand at 621.371 kb on - strand at 621.371 kb on - strand at 621.371 kb on - strand at 621.371 kb on - strand at 621.371 kb on - strand at 621.371 kb on - strand at 621.371 kb on - strand at 621.371 kb on - strand at 621.602 kb on + strand, within GFF577 at 622.036 kb on + strand, within GFF577 at 622.036 kb on + strand, within GFF577 at 622.037 kb on - strand, within GFF577 at 622.328 kb on + strand at 622.328 kb on + strand at 622.329 kb on - strand at 622.329 kb on - strand at 622.329 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 619,969 + GFF574 0.49 +0.1 619,970 - GFF574 0.50 +0.7 620,008 - GFF574 0.58 -0.2 620,026 - GFF574 0.62 +0.2 620,134 - GFF574 0.85 -0.5 620,165 + -0.6 620,165 + -0.7 620,165 + -0.2 620,166 - +0.3 620,166 - +0.8 620,166 - -0.2 620,166 - +0.7 620,188 + +0.5 620,188 + +1.7 620,188 + -0.3 620,188 + +0.3 620,188 + -0.3 620,188 + +0.3 620,188 + -0.0 620,188 + +1.0 620,188 + +0.4 620,188 + -2.4 620,188 + +0.5 620,188 + -0.4 620,188 + -0.1 620,188 + +0.3 620,188 + -1.2 620,188 + -0.0 620,188 + -0.7 620,188 + +0.3 620,188 + -0.3 620,189 - -0.6 620,189 - +0.1 620,189 - +0.1 620,189 - +0.4 620,189 - -0.2 620,189 - +0.2 620,189 - +0.3 620,189 - -0.1 620,189 - -1.1 620,189 - -0.1 620,189 - -0.4 620,189 - -0.5 620,189 - -0.8 620,210 + -1.1 620,210 + -0.4 620,210 + -1.4 620,210 + -0.4 620,210 + -0.2 620,210 + -0.6 620,210 + -1.1 620,210 + -2.9 620,210 + -0.6 620,210 + -2.6 620,211 - -1.0 620,211 - -0.8 620,211 - -1.6 620,211 - +0.3 620,211 - +0.7 620,211 - -1.4 620,211 - -0.1 620,211 - +0.1 620,211 - -0.8 620,211 - -0.5 620,211 - +2.0 620,211 - -1.6 620,211 - +0.1 620,211 - -0.4 620,314 + GFF575 0.16 +0.3 620,314 + GFF575 0.16 -1.1 620,314 + GFF575 0.16 -2.7 620,314 + GFF575 0.16 -1.6 620,315 - GFF575 0.16 -1.6 620,315 - GFF575 0.16 -1.1 620,468 - GFF575 0.37 -1.6 620,468 - GFF575 0.37 -0.4 620,614 + GFF575 0.58 -1.7 620,614 + GFF575 0.58 -0.6 620,614 + GFF575 0.58 +0.3 620,614 + GFF575 0.58 -0.3 620,614 + GFF575 0.58 -1.3 620,614 + GFF575 0.58 -2.1 620,614 + GFF575 0.58 -2.2 620,614 + GFF575 0.58 -2.1 620,614 + GFF575 0.58 +0.5 620,614 + GFF575 0.58 -1.9 620,614 + GFF575 0.58 -0.3 620,614 + GFF575 0.58 -1.7 620,615 - GFF575 0.58 -2.8 620,615 - GFF575 0.58 +0.5 620,615 - GFF575 0.58 -1.4 620,615 - GFF575 0.58 -1.2 620,615 - GFF575 0.58 -2.3 620,615 - GFF575 0.58 -2.1 620,615 - GFF575 0.58 -0.8 620,615 - GFF575 0.58 -1.8 620,615 - GFF575 0.58 -2.7 620,615 - GFF575 0.58 +0.4 620,615 - GFF575 0.58 +1.3 620,615 - GFF575 0.58 -1.2 620,615 - GFF575 0.58 -1.3 620,615 - GFF575 0.58 -3.0 620,615 - GFF575 0.58 -0.3 620,615 - GFF575 0.58 -0.3 620,615 - GFF575 0.58 -2.7 620,615 - GFF575 0.58 -3.4 620,615 - GFF575 0.58 -2.7 620,773 + GFF575 0.80 -2.2 620,773 + GFF575 0.80 -2.7 620,774 - GFF575 0.80 -0.6 620,774 - GFF575 0.80 -0.9 620,774 - GFF575 0.80 -0.7 620,824 + GFF575 0.87 -1.5 620,824 + GFF575 0.87 -1.7 620,824 + GFF575 0.87 +0.1 620,824 + GFF575 0.87 -1.2 620,824 + GFF575 0.87 -2.7 620,824 + GFF575 0.87 -0.9 620,824 + GFF575 0.87 -0.3 620,824 + GFF575 0.87 -0.4 620,825 - GFF575 0.87 -1.9 620,825 - GFF575 0.87 -1.2 620,825 - GFF575 0.87 -0.8 620,825 - GFF575 0.87 -0.9 620,825 - GFF575 0.87 +0.3 620,825 - GFF575 0.87 -0.1 620,825 - GFF575 0.87 -1.1 620,825 - GFF575 0.87 -1.6 621,283 + GFF576 0.79 -1.3 621,283 + GFF576 0.79 -1.7 621,283 + GFF576 0.79 -1.3 621,283 + GFF576 0.79 -0.7 621,283 + GFF576 0.79 -1.7 621,284 - GFF576 0.80 -1.9 621,284 - GFF576 0.80 -2.2 621,284 - GFF576 0.80 -1.4 621,370 + +0.5 621,370 + -0.8 621,370 + +0.8 621,370 + -0.8 621,370 + -0.7 621,370 + -0.2 621,370 + -0.5 621,370 + -2.2 621,370 + +0.1 621,371 - +0.4 621,371 - -0.7 621,371 - -1.1 621,371 - -0.7 621,371 - -0.7 621,371 - -1.2 621,371 - +0.3 621,371 - +0.0 621,371 - -1.0 621,371 - +1.0 621,371 - -1.1 621,371 - -0.5 621,602 + GFF577 0.24 -0.7 622,036 + GFF577 0.71 -0.2 622,036 + GFF577 0.71 -2.7 622,037 - GFF577 0.72 -1.8 622,328 + -1.4 622,328 + +1.1 622,329 - +0.8 622,329 - -1.9 622,329 - +0.1
Or see this region's nucleotide sequence