Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5193 and GFF5194 are separated by 12 nucleotides GFF5194 and GFF5195 overlap by 4 nucleotides
GFF5193 - Galactofuranose ABC transporter, substrate-binding protein, at 51,705 to 52,670
GFF5193
GFF5194 - L-arabinose ABC transporter, substrate-binding protein AraF, at 52,683 to 53,753
GFF5194
GFF5195 - L-arabinose ABC transporter, ATP-binding protein AraG, at 53,750 to 55,321
GFF5195
Position (kb) 52
53
54 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 51.948 kb on + strand, within GFF5193 at 51.948 kb on + strand, within GFF5193 at 52.090 kb on - strand, within GFF5193 at 52.090 kb on - strand, within GFF5193 at 52.666 kb on - strand at 52.842 kb on + strand, within GFF5194 at 52.861 kb on - strand, within GFF5194 at 52.861 kb on - strand, within GFF5194 at 53.064 kb on + strand, within GFF5194 at 53.064 kb on + strand, within GFF5194 at 53.208 kb on + strand, within GFF5194 at 53.238 kb on + strand, within GFF5194 at 53.358 kb on + strand, within GFF5194 at 53.358 kb on + strand, within GFF5194 at 53.358 kb on + strand, within GFF5194 at 53.359 kb on - strand, within GFF5194 at 53.359 kb on - strand, within GFF5194 at 53.634 kb on + strand, within GFF5194 at 53.634 kb on + strand, within GFF5194 at 53.635 kb on - strand, within GFF5194 at 53.665 kb on - strand at 53.730 kb on + strand at 53.730 kb on + strand at 53.730 kb on + strand at 53.730 kb on + strand at 53.951 kb on + strand, within GFF5195 at 53.952 kb on - strand, within GFF5195 at 53.952 kb on - strand, within GFF5195 at 54.134 kb on + strand, within GFF5195 at 54.134 kb on + strand, within GFF5195
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 51,948 + GFF5193 0.25 -0.3 51,948 + GFF5193 0.25 -1.2 52,090 - GFF5193 0.40 -0.3 52,090 - GFF5193 0.40 +0.0 52,666 - -0.7 52,842 + GFF5194 0.15 +0.0 52,861 - GFF5194 0.17 +2.4 52,861 - GFF5194 0.17 -1.4 53,064 + GFF5194 0.36 -0.6 53,064 + GFF5194 0.36 +0.0 53,208 + GFF5194 0.49 +0.3 53,238 + GFF5194 0.52 +1.4 53,358 + GFF5194 0.63 -2.8 53,358 + GFF5194 0.63 +0.2 53,358 + GFF5194 0.63 -0.4 53,359 - GFF5194 0.63 +0.5 53,359 - GFF5194 0.63 +0.2 53,634 + GFF5194 0.89 +0.3 53,634 + GFF5194 0.89 -0.5 53,635 - GFF5194 0.89 +0.2 53,665 - -0.0 53,730 + -0.7 53,730 + +0.9 53,730 + -0.5 53,730 + -0.3 53,951 + GFF5195 0.13 -0.0 53,952 - GFF5195 0.13 +0.1 53,952 - GFF5195 0.13 -0.2 54,134 + GFF5195 0.24 +0.4 54,134 + GFF5195 0.24 +1.6
Or see this region's nucleotide sequence