Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5147 and GFF5148 are separated by 25 nucleotides
GFF5147 - Alpha-ketoglutarate-dependent taurine dioxygenase (EC 1.14.11.17), at 309 to 1,205
GFF5147
GFF5148 - Taurine ABC transporter, substrate-binding protein TauA, at 1,231 to 2,241
GFF5148
Position (kb) 0
1
2 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 0.151 kb on + strand at 0.152 kb on - strand at 0.152 kb on - strand at 0.180 kb on - strand at 0.180 kb on - strand at 0.180 kb on - strand at 0.242 kb on - strand at 0.242 kb on - strand at 0.251 kb on + strand at 0.251 kb on + strand at 0.251 kb on + strand at 0.251 kb on + strand at 0.251 kb on + strand at 0.251 kb on + strand at 0.251 kb on + strand at 0.251 kb on + strand at 0.252 kb on - strand at 0.252 kb on - strand at 0.252 kb on - strand at 0.252 kb on - strand at 0.256 kb on + strand at 0.256 kb on + strand at 0.256 kb on + strand at 0.256 kb on + strand at 0.256 kb on + strand at 0.256 kb on + strand at 0.256 kb on + strand at 0.256 kb on + strand at 0.256 kb on + strand at 0.256 kb on + strand at 0.256 kb on + strand at 0.257 kb on - strand at 0.257 kb on - strand at 0.257 kb on - strand at 0.257 kb on - strand at 0.257 kb on - strand at 0.257 kb on - strand at 0.257 kb on - strand at 0.257 kb on - strand at 0.257 kb on - strand at 0.296 kb on + strand at 0.296 kb on + strand at 0.296 kb on + strand at 0.296 kb on + strand at 0.296 kb on + strand at 0.296 kb on + strand at 0.296 kb on + strand at 0.296 kb on + strand at 0.296 kb on + strand at 0.296 kb on + strand at 0.296 kb on + strand at 0.296 kb on + strand at 0.296 kb on + strand at 0.296 kb on + strand at 0.296 kb on + strand at 0.296 kb on + strand at 0.297 kb on - strand at 0.297 kb on - strand at 0.297 kb on - strand at 0.297 kb on - strand at 0.297 kb on - strand at 0.297 kb on - strand at 0.297 kb on - strand at 0.297 kb on - strand at 0.297 kb on - strand at 0.297 kb on - strand at 0.367 kb on + strand at 0.367 kb on + strand at 0.367 kb on + strand at 0.367 kb on + strand at 0.367 kb on + strand at 0.368 kb on - strand at 0.368 kb on - strand at 0.368 kb on - strand at 0.409 kb on + strand, within GFF5147 at 0.409 kb on + strand, within GFF5147 at 0.409 kb on + strand, within GFF5147 at 0.409 kb on + strand, within GFF5147 at 0.409 kb on + strand, within GFF5147 at 0.410 kb on - strand, within GFF5147 at 0.410 kb on - strand, within GFF5147 at 0.410 kb on - strand, within GFF5147 at 0.410 kb on - strand, within GFF5147 at 0.410 kb on - strand, within GFF5147 at 0.410 kb on - strand, within GFF5147 at 0.484 kb on + strand, within GFF5147 at 0.485 kb on - strand, within GFF5147 at 0.657 kb on + strand, within GFF5147 at 0.657 kb on + strand, within GFF5147 at 0.657 kb on + strand, within GFF5147 at 0.657 kb on + strand, within GFF5147 at 0.657 kb on + strand, within GFF5147 at 0.658 kb on - strand, within GFF5147 at 0.658 kb on - strand, within GFF5147 at 0.658 kb on - strand, within GFF5147 at 0.658 kb on - strand, within GFF5147 at 0.658 kb on - strand, within GFF5147 at 0.658 kb on - strand, within GFF5147 at 0.678 kb on + strand, within GFF5147 at 0.678 kb on + strand, within GFF5147 at 0.678 kb on + strand, within GFF5147 at 0.678 kb on + strand, within GFF5147 at 0.679 kb on - strand, within GFF5147 at 0.679 kb on - strand, within GFF5147 at 0.679 kb on - strand, within GFF5147 at 0.679 kb on - strand, within GFF5147 at 0.679 kb on - strand, within GFF5147 at 0.679 kb on - strand, within GFF5147 at 0.679 kb on - strand, within GFF5147 at 0.831 kb on + strand, within GFF5147 at 0.831 kb on + strand, within GFF5147 at 0.831 kb on + strand, within GFF5147 at 0.831 kb on + strand, within GFF5147 at 0.831 kb on + strand, within GFF5147 at 0.831 kb on + strand, within GFF5147 at 0.831 kb on + strand, within GFF5147 at 0.831 kb on + strand, within GFF5147 at 0.831 kb on + strand, within GFF5147 at 0.831 kb on + strand, within GFF5147 at 0.831 kb on + strand, within GFF5147 at 0.832 kb on - strand, within GFF5147 at 0.832 kb on - strand, within GFF5147 at 0.832 kb on - strand, within GFF5147 at 0.832 kb on - strand, within GFF5147 at 0.832 kb on - strand, within GFF5147 at 0.832 kb on - strand, within GFF5147 at 0.832 kb on - strand, within GFF5147 at 0.832 kb on - strand, within GFF5147 at 0.843 kb on + strand, within GFF5147 at 0.843 kb on + strand, within GFF5147 at 0.843 kb on + strand, within GFF5147 at 0.844 kb on - strand, within GFF5147 at 0.844 kb on - strand, within GFF5147 at 0.844 kb on - strand, within GFF5147 at 1.071 kb on + strand, within GFF5147 at 1.072 kb on - strand, within GFF5147 at 1.348 kb on + strand, within GFF5148 at 1.348 kb on + strand, within GFF5148 at 1.586 kb on - strand, within GFF5148 at 1.586 kb on - strand, within GFF5148 at 1.588 kb on + strand, within GFF5148 at 1.588 kb on + strand, within GFF5148 at 1.589 kb on - strand, within GFF5148 at 1.589 kb on - strand, within GFF5148 at 1.589 kb on - strand, within GFF5148 at 1.589 kb on - strand, within GFF5148 at 1.888 kb on + strand, within GFF5148 at 1.888 kb on + strand, within GFF5148 at 1.888 kb on + strand, within GFF5148 at 1.889 kb on - strand, within GFF5148 at 1.889 kb on - strand, within GFF5148 at 1.928 kb on - strand, within GFF5148 at 1.928 kb on - strand, within GFF5148 at 1.966 kb on + strand, within GFF5148 at 1.966 kb on + strand, within GFF5148 at 1.966 kb on + strand, within GFF5148 at 1.966 kb on + strand, within GFF5148 at 1.966 kb on + strand, within GFF5148 at 1.967 kb on - strand, within GFF5148 at 1.967 kb on - strand, within GFF5148 at 2.041 kb on + strand, within GFF5148 at 2.041 kb on + strand, within GFF5148 at 2.041 kb on + strand, within GFF5148 at 2.041 kb on + strand, within GFF5148 at 2.041 kb on + strand, within GFF5148 at 2.041 kb on + strand, within GFF5148 at 2.041 kb on + strand, within GFF5148 at 2.041 kb on + strand, within GFF5148 at 2.041 kb on + strand, within GFF5148 at 2.041 kb on + strand, within GFF5148 at 2.041 kb on + strand, within GFF5148 at 2.042 kb on - strand, within GFF5148 at 2.042 kb on - strand, within GFF5148 at 2.042 kb on - strand, within GFF5148 at 2.042 kb on - strand, within GFF5148 at 2.042 kb on - strand, within GFF5148 at 2.042 kb on - strand, within GFF5148 at 2.042 kb on - strand, within GFF5148 at 2.042 kb on - strand, within GFF5148 at 2.042 kb on - strand, within GFF5148 at 2.137 kb on + strand, within GFF5148 at 2.137 kb on + strand, within GFF5148 at 2.137 kb on + strand, within GFF5148 at 2.137 kb on + strand, within GFF5148 at 2.137 kb on + strand, within GFF5148 at 2.137 kb on + strand, within GFF5148 at 2.137 kb on + strand, within GFF5148 at 2.138 kb on - strand, within GFF5148 at 2.138 kb on - strand, within GFF5148 at 2.138 kb on - strand, within GFF5148 at 2.203 kb on + strand at 2.203 kb on + strand at 2.203 kb on + strand at 2.204 kb on - strand at 2.204 kb on - strand at 2.204 kb on - strand at 2.204 kb on - strand at 2.204 kb on - strand at 2.204 kb on - strand at 2.204 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 151 + -1.3 152 - -1.0 152 - -0.7 180 - -0.1 180 - +0.7 180 - -0.2 242 - -0.3 242 - +1.1 251 + +0.3 251 + +0.6 251 + -0.0 251 + -2.1 251 + -1.0 251 + +0.3 251 + -1.2 251 + -0.8 252 - +0.4 252 - -0.1 252 - +1.0 252 - -0.2 256 + -0.2 256 + -1.1 256 + +0.5 256 + -0.0 256 + +0.2 256 + +0.0 256 + +0.2 256 + -0.5 256 + +1.3 256 + -0.2 256 + -1.0 257 - -0.4 257 - -0.6 257 - -0.0 257 - -1.0 257 - -0.7 257 - -0.0 257 - -0.1 257 - -0.0 257 - -0.2 296 + +0.2 296 + -0.4 296 + -0.6 296 + -0.7 296 + -0.1 296 + -0.6 296 + -1.8 296 + +0.5 296 + -1.1 296 + -1.1 296 + +1.6 296 + -0.5 296 + -0.8 296 + +0.2 296 + +0.3 296 + -1.0 297 - +1.0 297 - -0.4 297 - -0.3 297 - +1.0 297 - -1.0 297 - -1.0 297 - -0.7 297 - +0.2 297 - +0.3 297 - -0.4 367 + +0.4 367 + -0.4 367 + -0.7 367 + +0.0 367 + -0.1 368 - -0.1 368 - +0.3 368 - -1.0 409 + GFF5147 0.11 -0.6 409 + GFF5147 0.11 +0.0 409 + GFF5147 0.11 -0.5 409 + GFF5147 0.11 -0.7 409 + GFF5147 0.11 +0.1 410 - GFF5147 0.11 +0.6 410 - GFF5147 0.11 -0.0 410 - GFF5147 0.11 +0.0 410 - GFF5147 0.11 +0.3 410 - GFF5147 0.11 +0.2 410 - GFF5147 0.11 -0.2 484 + GFF5147 0.20 +0.2 485 - GFF5147 0.20 +0.4 657 + GFF5147 0.39 -0.4 657 + GFF5147 0.39 +0.6 657 + GFF5147 0.39 -0.0 657 + GFF5147 0.39 -2.5 657 + GFF5147 0.39 -0.0 658 - GFF5147 0.39 +0.1 658 - GFF5147 0.39 -0.2 658 - GFF5147 0.39 +0.0 658 - GFF5147 0.39 +0.1 658 - GFF5147 0.39 +0.0 658 - GFF5147 0.39 +0.1 678 + GFF5147 0.41 +0.9 678 + GFF5147 0.41 -0.8 678 + GFF5147 0.41 +0.3 678 + GFF5147 0.41 -0.1 679 - GFF5147 0.41 -0.4 679 - GFF5147 0.41 +1.9 679 - GFF5147 0.41 +0.3 679 - GFF5147 0.41 +0.5 679 - GFF5147 0.41 +0.6 679 - GFF5147 0.41 +0.3 679 - GFF5147 0.41 +1.4 831 + GFF5147 0.58 -1.5 831 + GFF5147 0.58 +0.0 831 + GFF5147 0.58 -1.1 831 + GFF5147 0.58 -0.5 831 + GFF5147 0.58 -1.5 831 + GFF5147 0.58 +0.1 831 + GFF5147 0.58 +0.0 831 + GFF5147 0.58 -0.0 831 + GFF5147 0.58 +0.3 831 + GFF5147 0.58 +0.0 831 + GFF5147 0.58 +1.6 832 - GFF5147 0.58 +0.4 832 - GFF5147 0.58 +0.1 832 - GFF5147 0.58 +1.0 832 - GFF5147 0.58 -0.5 832 - GFF5147 0.58 +0.6 832 - GFF5147 0.58 +1.6 832 - GFF5147 0.58 +0.6 832 - GFF5147 0.58 -1.5 843 + GFF5147 0.60 -0.5 843 + GFF5147 0.60 -0.9 843 + GFF5147 0.60 -0.9 844 - GFF5147 0.60 -0.5 844 - GFF5147 0.60 -0.2 844 - GFF5147 0.60 -0.6 1,071 + GFF5147 0.85 +0.2 1,072 - GFF5147 0.85 +0.8 1,348 + GFF5148 0.12 -0.2 1,348 + GFF5148 0.12 -0.4 1,586 - GFF5148 0.35 +0.5 1,586 - GFF5148 0.35 -0.4 1,588 + GFF5148 0.35 +0.2 1,588 + GFF5148 0.35 -0.5 1,589 - GFF5148 0.35 +1.1 1,589 - GFF5148 0.35 -0.7 1,589 - GFF5148 0.35 -1.6 1,589 - GFF5148 0.35 -0.4 1,888 + GFF5148 0.65 -0.7 1,888 + GFF5148 0.65 +0.7 1,888 + GFF5148 0.65 +0.1 1,889 - GFF5148 0.65 -0.1 1,889 - GFF5148 0.65 -0.2 1,928 - GFF5148 0.69 -0.3 1,928 - GFF5148 0.69 +3.0 1,966 + GFF5148 0.73 -0.8 1,966 + GFF5148 0.73 -0.2 1,966 + GFF5148 0.73 -0.5 1,966 + GFF5148 0.73 +1.4 1,966 + GFF5148 0.73 +0.2 1,967 - GFF5148 0.73 +0.1 1,967 - GFF5148 0.73 -0.6 2,041 + GFF5148 0.80 -0.1 2,041 + GFF5148 0.80 +0.5 2,041 + GFF5148 0.80 -0.3 2,041 + GFF5148 0.80 +0.1 2,041 + GFF5148 0.80 +0.3 2,041 + GFF5148 0.80 +0.7 2,041 + GFF5148 0.80 +0.8 2,041 + GFF5148 0.80 +0.9 2,041 + GFF5148 0.80 -0.9 2,041 + GFF5148 0.80 +0.6 2,041 + GFF5148 0.80 +0.5 2,042 - GFF5148 0.80 -1.0 2,042 - GFF5148 0.80 -1.8 2,042 - GFF5148 0.80 -2.1 2,042 - GFF5148 0.80 -0.0 2,042 - GFF5148 0.80 -0.3 2,042 - GFF5148 0.80 -1.2 2,042 - GFF5148 0.80 +0.0 2,042 - GFF5148 0.80 -2.3 2,042 - GFF5148 0.80 -0.9 2,137 + GFF5148 0.90 -0.3 2,137 + GFF5148 0.90 +0.0 2,137 + GFF5148 0.90 -0.5 2,137 + GFF5148 0.90 +0.5 2,137 + GFF5148 0.90 -0.4 2,137 + GFF5148 0.90 +3.8 2,137 + GFF5148 0.90 -0.1 2,138 - GFF5148 0.90 -0.5 2,138 - GFF5148 0.90 -0.3 2,138 - GFF5148 0.90 +0.8 2,203 + +0.6 2,203 + +0.6 2,203 + -0.3 2,204 - +0.3 2,204 - +0.4 2,204 - -0.4 2,204 - +0.1 2,204 - -1.1 2,204 - -1.5 2,204 - +0.4
Or see this region's nucleotide sequence