Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF501 and GFF502 are separated by 4 nucleotides GFF502 and GFF503 are separated by 17 nucleotides GFF503 and GFF504 overlap by 4 nucleotides GFF504 and GFF505 are separated by 24 nucleotides
GFF501 - hypothetical protein, at 544,464 to 544,769
GFF501
GFF502 - Hydrolase, at 544,774 to 545,505
GFF502
GFF503 - Ferrochelatase, protoheme ferro-lyase (EC 4.99.1.1), at 545,523 to 546,554
GFF503
GFF504 - SAM-dependent methyltransferases, at 546,551 to 547,399
GFF504
GFF505 - no description, at 547,424 to 547,996
GFF505
Position (kb)
545
546
547 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 544.578 kb on + strand, within GFF501 at 544.578 kb on + strand, within GFF501 at 544.578 kb on + strand, within GFF501 at 544.579 kb on - strand, within GFF501 at 544.704 kb on + strand, within GFF501 at 544.734 kb on + strand, within GFF501 at 544.734 kb on + strand, within GFF501 at 544.735 kb on - strand, within GFF501 at 544.735 kb on - strand, within GFF501 at 544.747 kb on - strand at 544.767 kb on + strand at 544.768 kb on - strand at 544.768 kb on - strand at 544.768 kb on - strand at 544.768 kb on - strand at 544.768 kb on - strand at 544.768 kb on - strand at 544.768 kb on - strand at 544.768 kb on - strand at 544.768 kb on - strand at 545.141 kb on + strand, within GFF502 at 545.141 kb on + strand, within GFF502 at 545.517 kb on + strand at 546.869 kb on + strand, within GFF504 at 546.869 kb on + strand, within GFF504 at 546.869 kb on + strand, within GFF504 at 546.869 kb on + strand, within GFF504 at 546.869 kb on + strand, within GFF504 at 546.870 kb on - strand, within GFF504 at 546.870 kb on - strand, within GFF504 at 546.870 kb on - strand, within GFF504 at 546.870 kb on - strand, within GFF504 at 546.870 kb on - strand, within GFF504 at 546.870 kb on - strand, within GFF504 at 546.875 kb on + strand, within GFF504 at 546.876 kb on - strand, within GFF504 at 546.876 kb on - strand, within GFF504 at 546.876 kb on - strand, within GFF504 at 546.930 kb on - strand, within GFF504 at 547.067 kb on + strand, within GFF504 at 547.067 kb on + strand, within GFF504 at 547.067 kb on + strand, within GFF504 at 547.067 kb on + strand, within GFF504 at 547.067 kb on + strand, within GFF504 at 547.067 kb on + strand, within GFF504 at 547.068 kb on - strand, within GFF504 at 547.068 kb on - strand, within GFF504 at 547.068 kb on - strand, within GFF504 at 547.068 kb on - strand, within GFF504 at 547.073 kb on + strand, within GFF504 at 547.115 kb on + strand, within GFF504 at 547.115 kb on + strand, within GFF504 at 547.115 kb on + strand, within GFF504 at 547.115 kb on + strand, within GFF504 at 547.116 kb on - strand, within GFF504 at 547.116 kb on - strand, within GFF504 at 547.154 kb on + strand, within GFF504 at 547.154 kb on + strand, within GFF504 at 547.155 kb on - strand, within GFF504 at 547.155 kb on - strand, within GFF504 at 547.275 kb on - strand, within GFF504 at 547.280 kb on + strand, within GFF504 at 547.280 kb on + strand, within GFF504 at 547.280 kb on + strand, within GFF504 at 547.280 kb on + strand, within GFF504 at 547.281 kb on - strand, within GFF504 at 547.281 kb on - strand, within GFF504 at 547.281 kb on - strand, within GFF504 at 547.373 kb on + strand at 547.373 kb on + strand at 547.374 kb on - strand at 547.374 kb on - strand at 547.374 kb on - strand at 547.374 kb on - strand at 547.479 kb on + strand at 547.479 kb on + strand at 547.479 kb on + strand at 547.480 kb on - strand at 547.533 kb on + strand, within GFF505 at 547.533 kb on + strand, within GFF505 at 547.533 kb on + strand, within GFF505 at 547.533 kb on + strand, within GFF505 at 547.534 kb on - strand, within GFF505 at 547.534 kb on - strand, within GFF505 at 547.549 kb on - strand, within GFF505 at 547.549 kb on - strand, within GFF505
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 544,578 + GFF501 0.37 -0.9 544,578 + GFF501 0.37 +1.8 544,578 + GFF501 0.37 -0.1 544,579 - GFF501 0.38 -0.6 544,704 + GFF501 0.78 -1.9 544,734 + GFF501 0.88 -0.5 544,734 + GFF501 0.88 +0.8 544,735 - GFF501 0.89 -1.3 544,735 - GFF501 0.89 -0.7 544,747 - -0.8 544,767 + -0.7 544,768 - +0.2 544,768 - +0.4 544,768 - -1.4 544,768 - -0.3 544,768 - +0.5 544,768 - +0.9 544,768 - -0.3 544,768 - -0.5 544,768 - -0.5 545,141 + GFF502 0.50 -0.4 545,141 + GFF502 0.50 -1.7 545,517 + -0.7 546,869 + GFF504 0.37 -0.9 546,869 + GFF504 0.37 -0.1 546,869 + GFF504 0.37 +1.3 546,869 + GFF504 0.37 +0.2 546,869 + GFF504 0.37 -0.5 546,870 - GFF504 0.38 -0.1 546,870 - GFF504 0.38 -0.1 546,870 - GFF504 0.38 +0.3 546,870 - GFF504 0.38 -0.3 546,870 - GFF504 0.38 -1.5 546,870 - GFF504 0.38 -1.0 546,875 + GFF504 0.38 -0.8 546,876 - GFF504 0.38 -0.7 546,876 - GFF504 0.38 +0.7 546,876 - GFF504 0.38 +0.0 546,930 - GFF504 0.45 +0.0 547,067 + GFF504 0.61 -1.3 547,067 + GFF504 0.61 +0.5 547,067 + GFF504 0.61 -1.4 547,067 + GFF504 0.61 -0.3 547,067 + GFF504 0.61 -2.2 547,067 + GFF504 0.61 -0.5 547,068 - GFF504 0.61 +1.6 547,068 - GFF504 0.61 -0.2 547,068 - GFF504 0.61 -0.3 547,068 - GFF504 0.61 +0.0 547,073 + GFF504 0.61 +0.3 547,115 + GFF504 0.66 -1.1 547,115 + GFF504 0.66 -1.3 547,115 + GFF504 0.66 -0.4 547,115 + GFF504 0.66 -0.1 547,116 - GFF504 0.67 -0.7 547,116 - GFF504 0.67 -0.3 547,154 + GFF504 0.71 +0.2 547,154 + GFF504 0.71 -2.1 547,155 - GFF504 0.71 -0.5 547,155 - GFF504 0.71 -0.0 547,275 - GFF504 0.85 -0.0 547,280 + GFF504 0.86 +0.5 547,280 + GFF504 0.86 -0.2 547,280 + GFF504 0.86 -0.5 547,280 + GFF504 0.86 -0.0 547,281 - GFF504 0.86 +0.5 547,281 - GFF504 0.86 -0.5 547,281 - GFF504 0.86 +0.2 547,373 + -0.6 547,373 + -0.8 547,374 - -0.1 547,374 - +0.4 547,374 - -0.7 547,374 - +0.8 547,479 + -0.3 547,479 + -0.6 547,479 + -0.3 547,480 - -0.4 547,533 + GFF505 0.19 -1.4 547,533 + GFF505 0.19 +0.3 547,533 + GFF505 0.19 -1.6 547,533 + GFF505 0.19 -0.8 547,534 - GFF505 0.19 -0.1 547,534 - GFF505 0.19 -0.5 547,549 - GFF505 0.22 -0.5 547,549 - GFF505 0.22 -1.9
Or see this region's nucleotide sequence