Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4971 and GFF4972 overlap by 8 nucleotides GFF4972 and GFF4973 overlap by 4 nucleotides GFF4973 and GFF4974 overlap by 4 nucleotides GFF4974 and GFF4975 are separated by 13 nucleotides
GFF4971 - ABC transporter, ATP-binding protein 2 (cluster 4, leucine/isoleucine/valine/benzoate), at 71,761 to 72,480
GFF4971
GFF4972 - Branched-chain amino acid transport ATP-binding protein LivG (TC 3.A.1.4.1), at 72,473 to 73,237
GFF4972
GFF4973 - Branched-chain amino acid transport system permease protein LivM (TC 3.A.1.4.1), at 73,234 to 74,217
GFF4973
GFF4974 - High-affinity branched-chain amino acid transport system permease protein LivH (TC 3.A.1.4.1), at 74,214 to 75,116
GFF4974
GFF4975 - Branched-chain amino acid ABC transporter, amino acid-binding protein (TC 3.A.1.4.1), at 75,130 to 76,347
GFF4975
Position (kb) 73
74
75 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 72.438 kb on - strand at 72.501 kb on + strand at 72.501 kb on + strand at 72.502 kb on - strand at 72.503 kb on - strand at 73.071 kb on + strand, within GFF4972 at 73.071 kb on + strand, within GFF4972 at 73.072 kb on - strand, within GFF4972 at 73.072 kb on - strand, within GFF4972 at 73.352 kb on + strand, within GFF4973 at 73.352 kb on + strand, within GFF4973 at 73.352 kb on + strand, within GFF4973 at 73.353 kb on - strand, within GFF4973 at 73.532 kb on + strand, within GFF4973 at 73.532 kb on + strand, within GFF4973 at 73.532 kb on + strand, within GFF4973 at 73.533 kb on - strand, within GFF4973 at 73.533 kb on - strand, within GFF4973 at 73.533 kb on - strand, within GFF4973 at 73.533 kb on - strand, within GFF4973 at 73.533 kb on - strand, within GFF4973 at 73.727 kb on + strand, within GFF4973 at 73.727 kb on + strand, within GFF4973 at 73.754 kb on + strand, within GFF4973 at 73.754 kb on + strand, within GFF4973 at 73.871 kb on + strand, within GFF4973 at 73.871 kb on + strand, within GFF4973 at 73.871 kb on + strand, within GFF4973 at 73.872 kb on - strand, within GFF4973 at 73.872 kb on - strand, within GFF4973 at 73.997 kb on + strand, within GFF4973 at 73.997 kb on + strand, within GFF4973 at 74.111 kb on + strand, within GFF4973 at 74.111 kb on + strand, within GFF4973 at 74.111 kb on + strand, within GFF4973 at 74.111 kb on + strand, within GFF4973 at 74.112 kb on - strand, within GFF4973 at 74.112 kb on - strand, within GFF4973 at 74.112 kb on - strand, within GFF4973 at 74.120 kb on + strand at 74.218 kb on - strand at 74.218 kb on - strand at 74.623 kb on + strand, within GFF4974 at 74.624 kb on - strand, within GFF4974 at 74.624 kb on - strand, within GFF4974 at 74.782 kb on + strand, within GFF4974 at 74.917 kb on + strand, within GFF4974 at 75.047 kb on - strand at 75.140 kb on + strand at 75.140 kb on + strand at 75.140 kb on + strand at 75.141 kb on - strand at 75.141 kb on - strand at 75.141 kb on - strand at 75.141 kb on - strand at 75.141 kb on - strand at 75.141 kb on - strand at 75.158 kb on + strand at 75.158 kb on + strand at 75.159 kb on - strand at 75.203 kb on + strand at 75.203 kb on + strand at 75.203 kb on + strand at 75.203 kb on + strand at 75.203 kb on + strand at 75.203 kb on + strand at 75.204 kb on - strand at 75.204 kb on - strand at 75.204 kb on - strand at 75.204 kb on - strand at 75.204 kb on - strand at 75.204 kb on - strand at 75.204 kb on - strand at 75.204 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 72,438 - +0.4 72,501 + -0.1 72,501 + -0.1 72,502 - +0.7 72,503 - -0.7 73,071 + GFF4972 0.78 +0.0 73,071 + GFF4972 0.78 +0.4 73,072 - GFF4972 0.78 -0.7 73,072 - GFF4972 0.78 -0.6 73,352 + GFF4973 0.12 +0.3 73,352 + GFF4973 0.12 +0.3 73,352 + GFF4973 0.12 -0.4 73,353 - GFF4973 0.12 -0.2 73,532 + GFF4973 0.30 -0.7 73,532 + GFF4973 0.30 +1.7 73,532 + GFF4973 0.30 +1.7 73,533 - GFF4973 0.30 -2.4 73,533 - GFF4973 0.30 -1.0 73,533 - GFF4973 0.30 -1.2 73,533 - GFF4973 0.30 -0.6 73,533 - GFF4973 0.30 -0.5 73,727 + GFF4973 0.50 -3.4 73,727 + GFF4973 0.50 -0.1 73,754 + GFF4973 0.53 -1.0 73,754 + GFF4973 0.53 -0.3 73,871 + GFF4973 0.65 +1.0 73,871 + GFF4973 0.65 +0.4 73,871 + GFF4973 0.65 +0.3 73,872 - GFF4973 0.65 +1.7 73,872 - GFF4973 0.65 -1.0 73,997 + GFF4973 0.78 -1.5 73,997 + GFF4973 0.78 -0.3 74,111 + GFF4973 0.89 -0.5 74,111 + GFF4973 0.89 +0.3 74,111 + GFF4973 0.89 -0.8 74,111 + GFF4973 0.89 -0.8 74,112 - GFF4973 0.89 -0.3 74,112 - GFF4973 0.89 -0.1 74,112 - GFF4973 0.89 -0.3 74,120 + +0.1 74,218 - +1.2 74,218 - -1.6 74,623 + GFF4974 0.45 -0.2 74,624 - GFF4974 0.45 -0.9 74,624 - GFF4974 0.45 -1.3 74,782 + GFF4974 0.63 -0.1 74,917 + GFF4974 0.78 +0.2 75,047 - +0.4 75,140 + -2.9 75,140 + +0.3 75,140 + -0.4 75,141 - -0.9 75,141 - -1.1 75,141 - +0.3 75,141 - +0.1 75,141 - -1.2 75,141 - -0.2 75,158 + +2.5 75,158 + +0.2 75,159 - -0.4 75,203 + -2.1 75,203 + +0.5 75,203 + -0.4 75,203 + +0.7 75,203 + -1.5 75,203 + +0.7 75,204 - +0.4 75,204 - -0.8 75,204 - -0.9 75,204 - -0.3 75,204 - -0.9 75,204 - -0.1 75,204 - -0.8 75,204 - +0.2
Or see this region's nucleotide sequence