Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4958 and GFF4959 overlap by 4 nucleotides GFF4959 and GFF4960 overlap by 4 nucleotides GFF4960 and GFF4961 overlap by 4 nucleotides
GFF4958 - ABC transporter, permease protein 1 (cluster 4, leucine/isoleucine/valine/benzoate), at 58,886 to 59,806
GFF4958
GFF4959 - ABC transporter, permease protein 2 (cluster 4, leucine/isoleucine/valine/benzoate), at 59,803 to 60,792
GFF4959
GFF4960 - ABC transporter, ATP-binding protein 1 (cluster 4, leucine/isoleucine/valine/benzoate), at 60,789 to 61,544
GFF4960
GFF4961 - ABC transporter, ATP-binding protein 2 (cluster 4, leucine/isoleucine/valine/benzoate), at 61,541 to 62,260
GFF4961
Position (kb) 59
60
61 Strain fitness (log2 ratio) -2
-1
0
1
2 at 58.885 kb on + strand at 58.886 kb on - strand at 58.886 kb on - strand at 58.886 kb on - strand at 59.361 kb on - strand, within GFF4958 at 59.556 kb on + strand, within GFF4958 at 59.557 kb on - strand, within GFF4958 at 59.557 kb on - strand, within GFF4958 at 59.557 kb on - strand, within GFF4958 at 59.744 kb on + strand at 59.745 kb on - strand at 59.745 kb on - strand at 59.745 kb on - strand at 59.745 kb on - strand at 59.824 kb on + strand at 59.824 kb on + strand at 59.825 kb on - strand at 59.899 kb on + strand at 59.899 kb on + strand at 59.899 kb on + strand at 60.019 kb on + strand, within GFF4959 at 60.145 kb on + strand, within GFF4959 at 60.146 kb on - strand, within GFF4959 at 60.184 kb on + strand, within GFF4959 at 60.185 kb on - strand, within GFF4959 at 60.230 kb on - strand, within GFF4959
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 58,885 + +0.0 58,886 - +0.7 58,886 - -0.2 58,886 - +0.2 59,361 - GFF4958 0.52 -1.2 59,556 + GFF4958 0.73 -0.2 59,557 - GFF4958 0.73 -2.1 59,557 - GFF4958 0.73 -0.3 59,557 - GFF4958 0.73 +0.2 59,744 + -0.9 59,745 - -0.4 59,745 - -0.0 59,745 - -0.6 59,745 - +0.1 59,824 + -1.7 59,824 + -0.5 59,825 - +0.2 59,899 + -0.6 59,899 + +2.0 59,899 + +1.0 60,019 + GFF4959 0.22 -0.6 60,145 + GFF4959 0.35 -1.0 60,146 - GFF4959 0.35 -0.4 60,184 + GFF4959 0.38 -0.3 60,185 - GFF4959 0.39 +0.3 60,230 - GFF4959 0.43 -1.6
Or see this region's nucleotide sequence