Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4864 and GFF4865 are separated by 11 nucleotides GFF4865 and GFF4866 are separated by 34 nucleotides GFF4866 and GFF4867 are separated by 526 nucleotides GFF4867 and GFF4868 are separated by 47 nucleotides
GFF4864 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase (EC 2.3.1.9), at 235,872 to 237,053
GFF4864
GFF4865 - Enoyl-CoA hydratase (EC 4.2.1.17), at 237,065 to 237,850
GFF4865
GFF4866 - Oxidoreductase, short-chain dehydrogenase/reductase family, at 237,885 to 238,667
GFF4866
GFF4867 - Enoyl-CoA hydratase (EC 4.2.1.17), at 239,194 to 239,496
GFF4867
GFF4868 - Type IV secretory pathway, VirD2 components (relaxase), at 239,544 to 241,526
GFF4868
Position (kb) 237
238
239 Strain fitness (log2 ratio) -2
-1
0
1
2 at 236.986 kb on - strand at 236.986 kb on - strand at 237.026 kb on + strand at 237.026 kb on + strand at 237.027 kb on - strand at 237.027 kb on - strand at 237.027 kb on - strand at 237.027 kb on - strand at 237.027 kb on - strand at 237.027 kb on - strand at 237.027 kb on - strand at 237.027 kb on - strand at 237.208 kb on + strand, within GFF4865 at 237.208 kb on + strand, within GFF4865 at 237.411 kb on + strand, within GFF4865 at 237.411 kb on + strand, within GFF4865 at 237.411 kb on + strand, within GFF4865 at 237.412 kb on - strand, within GFF4865 at 237.412 kb on - strand, within GFF4865 at 237.529 kb on + strand, within GFF4865 at 237.530 kb on - strand, within GFF4865 at 237.530 kb on - strand, within GFF4865 at 237.530 kb on - strand, within GFF4865 at 237.760 kb on + strand, within GFF4865 at 237.760 kb on + strand, within GFF4865 at 237.760 kb on + strand, within GFF4865 at 237.760 kb on + strand, within GFF4865 at 237.761 kb on - strand, within GFF4865 at 237.761 kb on - strand, within GFF4865 at 237.761 kb on - strand, within GFF4865 at 237.761 kb on - strand, within GFF4865 at 237.761 kb on - strand, within GFF4865 at 237.761 kb on - strand, within GFF4865 at 237.761 kb on - strand, within GFF4865 at 237.848 kb on + strand at 237.859 kb on + strand at 237.859 kb on + strand at 237.859 kb on + strand at 237.859 kb on + strand at 237.859 kb on + strand at 237.859 kb on + strand at 237.859 kb on + strand at 237.859 kb on + strand at 237.859 kb on + strand at 237.859 kb on + strand at 237.860 kb on - strand at 237.860 kb on - strand at 237.860 kb on - strand at 237.860 kb on - strand at 237.860 kb on - strand at 237.860 kb on - strand at 237.860 kb on - strand at 237.860 kb on - strand at 237.860 kb on - strand at 237.860 kb on - strand at 237.860 kb on - strand at 238.044 kb on + strand, within GFF4866 at 238.045 kb on - strand, within GFF4866 at 238.281 kb on + strand, within GFF4866 at 238.283 kb on + strand, within GFF4866 at 238.284 kb on - strand, within GFF4866 at 238.284 kb on - strand, within GFF4866 at 238.380 kb on + strand, within GFF4866 at 238.380 kb on + strand, within GFF4866 at 238.380 kb on + strand, within GFF4866 at 238.380 kb on + strand, within GFF4866 at 238.380 kb on + strand, within GFF4866 at 238.380 kb on + strand, within GFF4866 at 238.380 kb on + strand, within GFF4866 at 238.380 kb on + strand, within GFF4866 at 238.380 kb on + strand, within GFF4866 at 238.380 kb on + strand, within GFF4866 at 238.380 kb on + strand, within GFF4866 at 238.380 kb on + strand, within GFF4866 at 238.380 kb on + strand, within GFF4866 at 238.381 kb on - strand, within GFF4866 at 238.381 kb on - strand, within GFF4866 at 238.381 kb on - strand, within GFF4866 at 238.381 kb on - strand, within GFF4866 at 238.381 kb on - strand, within GFF4866 at 238.381 kb on - strand, within GFF4866 at 238.381 kb on - strand, within GFF4866 at 238.381 kb on - strand, within GFF4866 at 238.381 kb on - strand, within GFF4866 at 238.381 kb on - strand, within GFF4866 at 238.381 kb on - strand, within GFF4866 at 238.484 kb on + strand, within GFF4866 at 238.485 kb on - strand, within GFF4866 at 238.485 kb on - strand, within GFF4866 at 238.485 kb on - strand, within GFF4866 at 238.485 kb on - strand, within GFF4866 at 238.601 kb on + strand at 238.601 kb on + strand at 238.602 kb on - strand at 238.602 kb on - strand at 238.602 kb on - strand at 238.611 kb on + strand at 238.612 kb on - strand at 238.612 kb on - strand at 238.718 kb on - strand at 238.718 kb on - strand at 238.750 kb on + strand at 238.750 kb on + strand at 238.750 kb on + strand at 238.750 kb on + strand at 238.750 kb on + strand at 238.750 kb on + strand at 238.750 kb on + strand at 238.750 kb on + strand at 238.751 kb on - strand at 238.751 kb on - strand at 238.751 kb on - strand at 238.751 kb on - strand at 238.751 kb on - strand at 238.751 kb on - strand at 238.751 kb on - strand at 238.751 kb on - strand at 238.798 kb on + strand at 238.798 kb on + strand at 238.934 kb on + strand at 238.934 kb on + strand at 238.934 kb on + strand at 238.934 kb on + strand at 238.935 kb on - strand at 238.935 kb on - strand at 238.935 kb on - strand at 238.935 kb on - strand at 238.935 kb on - strand at 238.935 kb on - strand at 238.935 kb on - strand at 238.935 kb on - strand at 238.935 kb on - strand at 238.935 kb on - strand at 238.935 kb on - strand at 239.017 kb on + strand at 239.017 kb on + strand at 239.017 kb on + strand at 239.017 kb on + strand at 239.017 kb on + strand at 239.017 kb on + strand at 239.018 kb on - strand at 239.018 kb on - strand at 239.018 kb on - strand at 239.074 kb on + strand at 239.075 kb on - strand at 239.075 kb on - strand at 239.156 kb on + strand at 239.156 kb on + strand at 239.156 kb on + strand at 239.157 kb on - strand at 239.157 kb on - strand at 239.157 kb on - strand at 239.214 kb on - strand at 239.295 kb on + strand, within GFF4867 at 239.332 kb on + strand, within GFF4867 at 239.332 kb on + strand, within GFF4867 at 239.504 kb on + strand at 239.504 kb on + strand at 239.504 kb on + strand at 239.504 kb on + strand at 239.504 kb on + strand at 239.505 kb on - strand at 239.505 kb on - strand at 239.505 kb on - strand at 239.505 kb on - strand at 239.521 kb on + strand at 239.521 kb on + strand at 239.521 kb on + strand at 239.521 kb on + strand at 239.522 kb on - strand at 239.522 kb on - strand at 239.522 kb on - strand at 239.522 kb on - strand at 239.550 kb on - strand at 239.556 kb on + strand at 239.556 kb on + strand at 239.556 kb on + strand at 239.557 kb on - strand at 239.557 kb on - strand at 239.557 kb on - strand at 239.586 kb on + strand at 239.586 kb on + strand at 239.586 kb on + strand at 239.587 kb on - strand at 239.601 kb on + strand at 239.601 kb on + strand at 239.601 kb on + strand at 239.601 kb on + strand at 239.601 kb on + strand at 239.601 kb on + strand at 239.601 kb on + strand at 239.601 kb on + strand at 239.601 kb on + strand at 239.601 kb on + strand at 239.601 kb on + strand at 239.601 kb on + strand at 239.601 kb on + strand at 239.601 kb on + strand at 239.601 kb on + strand at 239.601 kb on + strand at 239.602 kb on - strand at 239.602 kb on - strand at 239.602 kb on - strand at 239.602 kb on - strand at 239.602 kb on - strand at 239.602 kb on - strand at 239.602 kb on - strand at 239.602 kb on - strand at 239.602 kb on - strand at 239.602 kb on - strand at 239.602 kb on - strand at 239.602 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 236,986 - -1.0 236,986 - -0.3 237,026 + -0.6 237,026 + -0.1 237,027 - +0.2 237,027 - -1.2 237,027 - -1.5 237,027 - +0.9 237,027 - -0.8 237,027 - +0.1 237,027 - -1.5 237,027 - -1.2 237,208 + GFF4865 0.18 -0.3 237,208 + GFF4865 0.18 +0.2 237,411 + GFF4865 0.44 +0.2 237,411 + GFF4865 0.44 -0.5 237,411 + GFF4865 0.44 +0.0 237,412 - GFF4865 0.44 -0.1 237,412 - GFF4865 0.44 +0.5 237,529 + GFF4865 0.59 -0.0 237,530 - GFF4865 0.59 -0.5 237,530 - GFF4865 0.59 -1.2 237,530 - GFF4865 0.59 +0.0 237,760 + GFF4865 0.88 -0.2 237,760 + GFF4865 0.88 -0.5 237,760 + GFF4865 0.88 -1.2 237,760 + GFF4865 0.88 -0.2 237,761 - GFF4865 0.89 +0.2 237,761 - GFF4865 0.89 +0.3 237,761 - GFF4865 0.89 -0.6 237,761 - GFF4865 0.89 +0.3 237,761 - GFF4865 0.89 +1.0 237,761 - GFF4865 0.89 +0.7 237,761 - GFF4865 0.89 -0.1 237,848 + +0.1 237,859 + +0.3 237,859 + +0.3 237,859 + +0.3 237,859 + +0.3 237,859 + -1.2 237,859 + -0.7 237,859 + +0.1 237,859 + -1.5 237,859 + -1.2 237,859 + -2.7 237,860 - +0.6 237,860 - -0.3 237,860 - -0.4 237,860 - -0.4 237,860 - +1.0 237,860 - -1.5 237,860 - -0.3 237,860 - +0.3 237,860 - -0.0 237,860 - +0.1 237,860 - -1.2 238,044 + GFF4866 0.20 +1.2 238,045 - GFF4866 0.20 +0.0 238,281 + GFF4866 0.51 -0.2 238,283 + GFF4866 0.51 +0.0 238,284 - GFF4866 0.51 -0.6 238,284 - GFF4866 0.51 +0.3 238,380 + GFF4866 0.63 +1.0 238,380 + GFF4866 0.63 -1.7 238,380 + GFF4866 0.63 -0.7 238,380 + GFF4866 0.63 -1.4 238,380 + GFF4866 0.63 +0.5 238,380 + GFF4866 0.63 -0.7 238,380 + GFF4866 0.63 -0.7 238,380 + GFF4866 0.63 -0.3 238,380 + GFF4866 0.63 +0.1 238,380 + GFF4866 0.63 +0.3 238,380 + GFF4866 0.63 -1.1 238,380 + GFF4866 0.63 +0.6 238,380 + GFF4866 0.63 -0.2 238,381 - GFF4866 0.63 -0.4 238,381 - GFF4866 0.63 +0.3 238,381 - GFF4866 0.63 -0.2 238,381 - GFF4866 0.63 +0.8 238,381 - GFF4866 0.63 +2.7 238,381 - GFF4866 0.63 +0.2 238,381 - GFF4866 0.63 -1.0 238,381 - GFF4866 0.63 +0.3 238,381 - GFF4866 0.63 -0.1 238,381 - GFF4866 0.63 +1.6 238,381 - GFF4866 0.63 -0.1 238,484 + GFF4866 0.77 -0.0 238,485 - GFF4866 0.77 -0.3 238,485 - GFF4866 0.77 +0.9 238,485 - GFF4866 0.77 +0.5 238,485 - GFF4866 0.77 -0.9 238,601 + -0.4 238,601 + -0.1 238,602 - +1.6 238,602 - -0.3 238,602 - -0.7 238,611 + -2.2 238,612 - -0.1 238,612 - -0.9 238,718 - +0.3 238,718 - +0.5 238,750 + -0.7 238,750 + +0.5 238,750 + -0.7 238,750 + +0.6 238,750 + +1.3 238,750 + -0.5 238,750 + +0.1 238,750 + +0.1 238,751 - -0.3 238,751 - -0.4 238,751 - -0.7 238,751 - -0.1 238,751 - +0.5 238,751 - -0.9 238,751 - -0.5 238,751 - +0.6 238,798 + -1.2 238,798 + -0.4 238,934 + +0.3 238,934 + -0.6 238,934 + +2.3 238,934 + +1.8 238,935 - +1.0 238,935 - +0.4 238,935 - -0.3 238,935 - -1.7 238,935 - -1.9 238,935 - -0.2 238,935 - +1.3 238,935 - +0.0 238,935 - +0.5 238,935 - -0.5 238,935 - -2.0 239,017 + -0.6 239,017 + -0.4 239,017 + +0.2 239,017 + -0.9 239,017 + -0.4 239,017 + +0.3 239,018 - -0.3 239,018 - -0.4 239,018 - +0.3 239,074 + -0.4 239,075 - -0.2 239,075 - -0.2 239,156 + -0.3 239,156 + +0.0 239,156 + -0.1 239,157 - -0.1 239,157 - -1.2 239,157 - +0.8 239,214 - -0.4 239,295 + GFF4867 0.33 +0.0 239,332 + GFF4867 0.46 -1.0 239,332 + GFF4867 0.46 +0.1 239,504 + -0.1 239,504 + -0.0 239,504 + +0.1 239,504 + +0.5 239,504 + -0.2 239,505 - -0.5 239,505 - -1.8 239,505 - +1.3 239,505 - -1.3 239,521 + -0.8 239,521 + +0.1 239,521 + -0.6 239,521 + +0.3 239,522 - +1.1 239,522 - -0.2 239,522 - +0.5 239,522 - -0.5 239,550 - -0.8 239,556 + +0.1 239,556 + +0.3 239,556 + +0.0 239,557 - -0.8 239,557 - -0.1 239,557 - +0.0 239,586 + +0.8 239,586 + +0.0 239,586 + -0.7 239,587 - +0.5 239,601 + -0.7 239,601 + -1.2 239,601 + +0.0 239,601 + +0.3 239,601 + -1.1 239,601 + -0.2 239,601 + -0.4 239,601 + +0.1 239,601 + -0.3 239,601 + -1.0 239,601 + +1.3 239,601 + -0.8 239,601 + +0.9 239,601 + -0.3 239,601 + +0.0 239,601 + -0.6 239,602 - +0.3 239,602 - +0.8 239,602 - +0.3 239,602 - +0.2 239,602 - -1.7 239,602 - +0.5 239,602 - +0.5 239,602 - +0.0 239,602 - -0.4 239,602 - -0.1 239,602 - -0.5 239,602 - +0.0
Or see this region's nucleotide sequence