Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF43 and GFF44 are separated by 46 nucleotides GFF44 and GFF45 are separated by 26 nucleotides GFF45 and GFF46 are separated by 103 nucleotides GFF46 and GFF47 are separated by 24 nucleotides
GFF43 - hypothetical protein, at 44,860 to 46,923
GFF43
GFF44 - hypothetical protein, at 46,970 to 47,146
GFF44
GFF45 - Phospholipase C (EC 3.1.4.3), at 47,173 to 49,449
GFF45
GFF46 - Transcriptional regulators of sugar metabolism, at 49,553 to 50,329
GFF46
GFF47 - DNA double-strand break repair Rad50 ATPase, at 50,354 to 52,993
GFF47
Position (kb)
47
48
49
50 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 46.174 kb on + strand, within GFF43 at 46.265 kb on + strand, within GFF43 at 46.265 kb on + strand, within GFF43 at 46.266 kb on - strand, within GFF43 at 46.266 kb on - strand, within GFF43 at 46.266 kb on - strand, within GFF43 at 46.266 kb on - strand, within GFF43 at 46.266 kb on - strand, within GFF43 at 46.457 kb on - strand, within GFF43 at 46.486 kb on + strand, within GFF43 at 46.487 kb on - strand, within GFF43 at 46.487 kb on - strand, within GFF43 at 46.487 kb on - strand, within GFF43 at 46.618 kb on + strand, within GFF43 at 46.619 kb on - strand, within GFF43 at 46.619 kb on - strand, within GFF43 at 46.663 kb on + strand, within GFF43 at 46.663 kb on + strand, within GFF43 at 46.663 kb on + strand, within GFF43 at 46.664 kb on - strand, within GFF43 at 46.664 kb on - strand, within GFF43 at 46.664 kb on - strand, within GFF43 at 46.783 kb on + strand at 46.783 kb on + strand at 46.783 kb on + strand at 46.783 kb on + strand at 46.784 kb on - strand at 46.784 kb on - strand at 46.786 kb on + strand at 46.786 kb on + strand at 46.786 kb on + strand at 46.787 kb on - strand at 46.787 kb on - strand at 46.787 kb on - strand at 46.885 kb on + strand at 46.885 kb on + strand at 46.886 kb on - strand at 46.886 kb on - strand at 46.886 kb on - strand at 46.886 kb on - strand at 46.886 kb on - strand at 47.001 kb on + strand, within GFF44 at 47.359 kb on + strand at 47.359 kb on + strand at 47.474 kb on - strand, within GFF45 at 47.517 kb on + strand, within GFF45 at 47.569 kb on + strand, within GFF45 at 47.569 kb on + strand, within GFF45 at 47.569 kb on + strand, within GFF45 at 47.569 kb on + strand, within GFF45 at 47.569 kb on + strand, within GFF45 at 47.569 kb on + strand, within GFF45 at 47.569 kb on + strand, within GFF45 at 47.569 kb on + strand, within GFF45 at 47.570 kb on - strand, within GFF45 at 47.570 kb on - strand, within GFF45 at 47.570 kb on - strand, within GFF45 at 47.570 kb on - strand, within GFF45 at 47.570 kb on - strand, within GFF45 at 47.570 kb on - strand, within GFF45 at 47.570 kb on - strand, within GFF45 at 47.608 kb on + strand, within GFF45 at 47.609 kb on - strand, within GFF45 at 47.609 kb on - strand, within GFF45 at 47.691 kb on + strand, within GFF45 at 47.691 kb on + strand, within GFF45 at 47.691 kb on + strand, within GFF45 at 47.692 kb on - strand, within GFF45 at 47.875 kb on + strand, within GFF45 at 47.875 kb on + strand, within GFF45 at 47.875 kb on + strand, within GFF45 at 47.875 kb on + strand, within GFF45 at 47.876 kb on - strand, within GFF45 at 47.938 kb on + strand, within GFF45 at 47.938 kb on + strand, within GFF45 at 47.938 kb on + strand, within GFF45 at 47.939 kb on - strand, within GFF45 at 47.939 kb on - strand, within GFF45 at 47.939 kb on - strand, within GFF45 at 47.964 kb on + strand, within GFF45 at 47.972 kb on - strand, within GFF45 at 48.013 kb on + strand, within GFF45 at 48.013 kb on + strand, within GFF45 at 48.014 kb on - strand, within GFF45 at 48.059 kb on - strand, within GFF45 at 48.059 kb on - strand, within GFF45 at 48.059 kb on - strand, within GFF45 at 48.059 kb on - strand, within GFF45 at 48.059 kb on - strand, within GFF45 at 48.094 kb on + strand, within GFF45 at 48.094 kb on + strand, within GFF45 at 48.095 kb on - strand, within GFF45 at 48.205 kb on + strand, within GFF45 at 48.205 kb on + strand, within GFF45 at 48.205 kb on + strand, within GFF45 at 48.206 kb on - strand, within GFF45 at 48.206 kb on - strand, within GFF45 at 48.250 kb on + strand, within GFF45 at 48.250 kb on + strand, within GFF45 at 48.250 kb on + strand, within GFF45 at 48.251 kb on - strand, within GFF45 at 48.251 kb on - strand, within GFF45 at 48.251 kb on - strand, within GFF45 at 48.340 kb on + strand, within GFF45 at 48.341 kb on - strand, within GFF45 at 48.343 kb on + strand, within GFF45 at 48.456 kb on + strand, within GFF45 at 48.456 kb on + strand, within GFF45 at 48.456 kb on + strand, within GFF45 at 48.456 kb on + strand, within GFF45 at 48.456 kb on + strand, within GFF45 at 48.456 kb on + strand, within GFF45 at 48.456 kb on + strand, within GFF45 at 48.456 kb on + strand, within GFF45 at 48.456 kb on + strand, within GFF45 at 48.457 kb on - strand, within GFF45 at 48.457 kb on - strand, within GFF45 at 48.457 kb on - strand, within GFF45 at 48.457 kb on - strand, within GFF45 at 48.457 kb on - strand, within GFF45 at 48.457 kb on - strand, within GFF45 at 48.457 kb on - strand, within GFF45 at 48.457 kb on - strand, within GFF45 at 48.457 kb on - strand, within GFF45 at 48.460 kb on + strand, within GFF45 at 48.460 kb on + strand, within GFF45 at 48.460 kb on + strand, within GFF45 at 48.461 kb on - strand, within GFF45 at 48.461 kb on - strand, within GFF45 at 48.532 kb on + strand, within GFF45 at 48.532 kb on + strand, within GFF45 at 48.532 kb on + strand, within GFF45 at 48.532 kb on + strand, within GFF45 at 48.532 kb on + strand, within GFF45 at 48.532 kb on + strand, within GFF45 at 48.532 kb on + strand, within GFF45 at 48.532 kb on + strand, within GFF45 at 48.533 kb on - strand, within GFF45 at 48.533 kb on - strand, within GFF45 at 48.533 kb on - strand, within GFF45 at 48.533 kb on - strand, within GFF45 at 48.533 kb on - strand, within GFF45 at 48.533 kb on - strand, within GFF45 at 48.533 kb on - strand, within GFF45 at 48.533 kb on - strand, within GFF45 at 48.533 kb on - strand, within GFF45 at 48.649 kb on + strand, within GFF45 at 48.859 kb on + strand, within GFF45 at 48.860 kb on - strand, within GFF45 at 48.956 kb on - strand, within GFF45 at 48.956 kb on - strand, within GFF45 at 48.956 kb on - strand, within GFF45 at 48.956 kb on - strand, within GFF45 at 48.991 kb on + strand, within GFF45 at 48.992 kb on - strand, within GFF45 at 48.992 kb on - strand, within GFF45 at 48.992 kb on - strand, within GFF45 at 48.992 kb on - strand, within GFF45 at 48.992 kb on - strand, within GFF45 at 49.057 kb on + strand, within GFF45 at 49.057 kb on + strand, within GFF45 at 49.057 kb on + strand, within GFF45 at 49.057 kb on + strand, within GFF45 at 49.057 kb on + strand, within GFF45 at 49.058 kb on - strand, within GFF45 at 49.058 kb on - strand, within GFF45 at 49.058 kb on - strand, within GFF45 at 49.162 kb on + strand, within GFF45 at 49.187 kb on - strand, within GFF45 at 49.187 kb on - strand, within GFF45 at 49.187 kb on - strand, within GFF45 at 49.187 kb on - strand, within GFF45 at 49.333 kb on + strand at 49.333 kb on + strand at 49.333 kb on + strand at 49.334 kb on - strand at 49.334 kb on - strand at 49.369 kb on + strand at 49.370 kb on - strand at 49.370 kb on - strand at 49.370 kb on - strand at 49.373 kb on - strand at 49.511 kb on - strand at 49.511 kb on - strand at 50.222 kb on + strand, within GFF46 at 50.222 kb on + strand, within GFF46 at 50.223 kb on - strand, within GFF46 at 50.356 kb on - strand at 50.445 kb on + strand at 50.445 kb on + strand at 50.445 kb on + strand at 50.445 kb on + strand at 50.445 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 46,174 + GFF43 0.64 -0.5 46,265 + GFF43 0.68 -0.6 46,265 + GFF43 0.68 -0.3 46,266 - GFF43 0.68 -1.3 46,266 - GFF43 0.68 -0.8 46,266 - GFF43 0.68 +0.5 46,266 - GFF43 0.68 -1.3 46,266 - GFF43 0.68 -0.4 46,457 - GFF43 0.77 -1.3 46,486 + GFF43 0.79 -0.3 46,487 - GFF43 0.79 -0.7 46,487 - GFF43 0.79 +0.5 46,487 - GFF43 0.79 -0.2 46,618 + GFF43 0.85 +0.1 46,619 - GFF43 0.85 +0.3 46,619 - GFF43 0.85 +0.8 46,663 + GFF43 0.87 -0.1 46,663 + GFF43 0.87 -1.5 46,663 + GFF43 0.87 -0.0 46,664 - GFF43 0.87 -0.6 46,664 - GFF43 0.87 +0.7 46,664 - GFF43 0.87 -0.9 46,783 + -0.5 46,783 + -0.7 46,783 + -0.3 46,783 + -0.7 46,784 - +0.7 46,784 - +1.3 46,786 + -1.3 46,786 + -0.2 46,786 + +0.1 46,787 - +0.2 46,787 - +0.3 46,787 - -0.8 46,885 + -0.7 46,885 + -0.6 46,886 - +0.2 46,886 - +0.3 46,886 - -0.3 46,886 - +0.1 46,886 - -0.2 47,001 + GFF44 0.18 -0.2 47,359 + -1.0 47,359 + -0.2 47,474 - GFF45 0.13 -0.2 47,517 + GFF45 0.15 -0.0 47,569 + GFF45 0.17 -0.6 47,569 + GFF45 0.17 +0.0 47,569 + GFF45 0.17 -0.5 47,569 + GFF45 0.17 -0.0 47,569 + GFF45 0.17 -0.7 47,569 + GFF45 0.17 -0.5 47,569 + GFF45 0.17 -2.0 47,569 + GFF45 0.17 -0.2 47,570 - GFF45 0.17 +0.8 47,570 - GFF45 0.17 +0.7 47,570 - GFF45 0.17 +0.2 47,570 - GFF45 0.17 -0.1 47,570 - GFF45 0.17 -0.5 47,570 - GFF45 0.17 -0.3 47,570 - GFF45 0.17 +0.4 47,608 + GFF45 0.19 -1.7 47,609 - GFF45 0.19 +0.3 47,609 - GFF45 0.19 +0.6 47,691 + GFF45 0.23 +2.3 47,691 + GFF45 0.23 -1.3 47,691 + GFF45 0.23 -0.4 47,692 - GFF45 0.23 -0.8 47,875 + GFF45 0.31 -0.6 47,875 + GFF45 0.31 +0.5 47,875 + GFF45 0.31 +0.3 47,875 + GFF45 0.31 -0.2 47,876 - GFF45 0.31 +0.2 47,938 + GFF45 0.34 -0.8 47,938 + GFF45 0.34 -0.4 47,938 + GFF45 0.34 -0.4 47,939 - GFF45 0.34 -0.4 47,939 - GFF45 0.34 -0.5 47,939 - GFF45 0.34 +0.4 47,964 + GFF45 0.35 -1.0 47,972 - GFF45 0.35 +1.2 48,013 + GFF45 0.37 -0.6 48,013 + GFF45 0.37 -0.2 48,014 - GFF45 0.37 +0.5 48,059 - GFF45 0.39 -0.7 48,059 - GFF45 0.39 +0.1 48,059 - GFF45 0.39 -2.2 48,059 - GFF45 0.39 -0.3 48,059 - GFF45 0.39 -0.3 48,094 + GFF45 0.40 -0.5 48,094 + GFF45 0.40 -0.3 48,095 - GFF45 0.40 +0.3 48,205 + GFF45 0.45 -0.1 48,205 + GFF45 0.45 +0.6 48,205 + GFF45 0.45 -0.3 48,206 - GFF45 0.45 +0.7 48,206 - GFF45 0.45 -0.7 48,250 + GFF45 0.47 -0.5 48,250 + GFF45 0.47 +1.0 48,250 + GFF45 0.47 +0.1 48,251 - GFF45 0.47 -0.7 48,251 - GFF45 0.47 -0.2 48,251 - GFF45 0.47 +1.8 48,340 + GFF45 0.51 -0.6 48,341 - GFF45 0.51 +0.4 48,343 + GFF45 0.51 -2.3 48,456 + GFF45 0.56 -1.1 48,456 + GFF45 0.56 -0.3 48,456 + GFF45 0.56 -0.2 48,456 + GFF45 0.56 -0.4 48,456 + GFF45 0.56 -1.3 48,456 + GFF45 0.56 -0.1 48,456 + GFF45 0.56 -0.9 48,456 + GFF45 0.56 -1.7 48,456 + GFF45 0.56 +0.3 48,457 - GFF45 0.56 -2.7 48,457 - GFF45 0.56 -0.4 48,457 - GFF45 0.56 -2.3 48,457 - GFF45 0.56 +0.5 48,457 - GFF45 0.56 +0.4 48,457 - GFF45 0.56 -0.6 48,457 - GFF45 0.56 -1.2 48,457 - GFF45 0.56 -1.3 48,457 - GFF45 0.56 -0.1 48,460 + GFF45 0.57 -1.9 48,460 + GFF45 0.57 -1.1 48,460 + GFF45 0.57 -0.7 48,461 - GFF45 0.57 -0.0 48,461 - GFF45 0.57 +1.6 48,532 + GFF45 0.60 +0.3 48,532 + GFF45 0.60 -1.3 48,532 + GFF45 0.60 -1.0 48,532 + GFF45 0.60 -0.8 48,532 + GFF45 0.60 -0.1 48,532 + GFF45 0.60 +0.1 48,532 + GFF45 0.60 +0.5 48,532 + GFF45 0.60 +0.7 48,533 - GFF45 0.60 -1.3 48,533 - GFF45 0.60 -1.1 48,533 - GFF45 0.60 -0.8 48,533 - GFF45 0.60 -1.2 48,533 - GFF45 0.60 -0.3 48,533 - GFF45 0.60 +0.7 48,533 - GFF45 0.60 -0.5 48,533 - GFF45 0.60 +0.8 48,533 - GFF45 0.60 -1.7 48,649 + GFF45 0.65 -0.0 48,859 + GFF45 0.74 -0.3 48,860 - GFF45 0.74 +0.0 48,956 - GFF45 0.78 -0.0 48,956 - GFF45 0.78 -2.9 48,956 - GFF45 0.78 -0.2 48,956 - GFF45 0.78 +1.5 48,991 + GFF45 0.80 -0.7 48,992 - GFF45 0.80 +0.2 48,992 - GFF45 0.80 +0.9 48,992 - GFF45 0.80 -0.4 48,992 - GFF45 0.80 -0.0 48,992 - GFF45 0.80 -0.4 49,057 + GFF45 0.83 +1.5 49,057 + GFF45 0.83 -0.3 49,057 + GFF45 0.83 +0.1 49,057 + GFF45 0.83 +1.5 49,057 + GFF45 0.83 -1.3 49,058 - GFF45 0.83 -1.1 49,058 - GFF45 0.83 -0.2 49,058 - GFF45 0.83 -0.7 49,162 + GFF45 0.87 -0.5 49,187 - GFF45 0.88 -0.9 49,187 - GFF45 0.88 -2.3 49,187 - GFF45 0.88 -0.5 49,187 - GFF45 0.88 -0.3 49,333 + -1.3 49,333 + -0.7 49,333 + -0.9 49,334 - -0.4 49,334 - +0.4 49,369 + -1.2 49,370 - +0.0 49,370 - -0.5 49,370 - -0.1 49,373 - -0.6 49,511 - -1.3 49,511 - -0.2 50,222 + GFF46 0.86 +1.3 50,222 + GFF46 0.86 +1.8 50,223 - GFF46 0.86 +0.6 50,356 - -0.5 50,445 + -0.1 50,445 + -1.1 50,445 + -0.3 50,445 + -0.1 50,445 + +1.3
Or see this region's nucleotide sequence