Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4056 and GFF4057 overlap by 4 nucleotides GFF4057 and GFF4058 overlap by 4 nucleotides
GFF4056 - Copper/silver efflux RND transporter, transmembrane protein CusA, at 266,783 to 269,947
GFF4056
GFF4057 - Copper/silver efflux RND transporter, membrane fusion protein CusB, at 269,944 to 271,647
GFF4057
GFF4058 - Copper/silver efflux RND transporter, outer membrane protein CusC, at 271,644 to 272,999
GFF4058
Position (kb)
269
270
271
272 Strain fitness (log2 ratio)
-1
0
1 at 268.974 kb on + strand, within GFF4056 at 269.263 kb on - strand, within GFF4056 at 269.263 kb on - strand, within GFF4056 at 269.529 kb on + strand, within GFF4056 at 269.530 kb on - strand, within GFF4056 at 269.530 kb on - strand, within GFF4056 at 269.667 kb on + strand at 269.667 kb on + strand at 269.668 kb on - strand at 269.694 kb on + strand at 269.790 kb on + strand at 269.863 kb on - strand at 269.863 kb on - strand at 270.271 kb on - strand, within GFF4057 at 271.371 kb on - strand, within GFF4057 at 271.577 kb on + strand at 271.577 kb on + strand at 271.578 kb on - strand at 271.578 kb on - strand at 271.900 kb on + strand, within GFF4058 at 271.900 kb on + strand, within GFF4058 at 272.055 kb on + strand, within GFF4058 at 272.120 kb on - strand, within GFF4058 at 272.120 kb on - strand, within GFF4058 at 272.123 kb on + strand, within GFF4058 at 272.123 kb on + strand, within GFF4058 at 272.123 kb on + strand, within GFF4058 at 272.123 kb on + strand, within GFF4058 at 272.522 kb on - strand, within GFF4058 at 272.577 kb on - strand, within GFF4058
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 268,974 + GFF4056 0.69 +1.4 269,263 - GFF4056 0.78 +0.1 269,263 - GFF4056 0.78 -0.7 269,529 + GFF4056 0.87 -0.3 269,530 - GFF4056 0.87 +1.1 269,530 - GFF4056 0.87 -0.8 269,667 + -0.5 269,667 + -0.3 269,668 - +0.4 269,694 + +0.1 269,790 + -0.9 269,863 - +0.1 269,863 - -1.3 270,271 - GFF4057 0.19 -0.6 271,371 - GFF4057 0.84 -0.7 271,577 + -0.1 271,577 + -0.8 271,578 - -0.2 271,578 - +0.3 271,900 + GFF4058 0.19 -0.5 271,900 + GFF4058 0.19 -0.7 272,055 + GFF4058 0.30 -0.5 272,120 - GFF4058 0.35 -1.3 272,120 - GFF4058 0.35 -0.3 272,123 + GFF4058 0.35 +0.0 272,123 + GFF4058 0.35 +0.3 272,123 + GFF4058 0.35 -0.3 272,123 + GFF4058 0.35 +0.2 272,522 - GFF4058 0.65 -1.3 272,577 - GFF4058 0.69 +1.3
Or see this region's nucleotide sequence