Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF377 and GFF378 are separated by 140 nucleotides GFF378 and GFF379 are separated by 7 nucleotides GFF379 and GFF380 overlap by 11 nucleotides
GFF377 - Carbamoyl-phosphate synthase small chain (EC 6.3.5.5), at 408,978 to 410,177
GFF377
GFF378 - RNA (nucleoside 2'-O)-methyltransferase, at 410,318 to 411,103
GFF378
GFF379 - Ribonuclease HII (EC 3.1.26.4), at 411,111 to 411,821
GFF379
GFF380 - Lipid-A-disaccharide synthase (EC 2.4.1.182), at 411,811 to 412,959
GFF380
Position (kb) 410
411
412 Strain fitness (log2 ratio) -2
-1
0
1 at 410.196 kb on - strand at 410.196 kb on - strand at 410.196 kb on - strand at 410.300 kb on + strand at 410.301 kb on - strand at 410.301 kb on - strand at 410.301 kb on - strand at 410.301 kb on - strand at 410.319 kb on + strand at 410.319 kb on + strand at 410.319 kb on + strand at 410.319 kb on + strand at 410.319 kb on + strand at 410.319 kb on + strand at 410.320 kb on - strand at 410.320 kb on - strand at 410.320 kb on - strand at 410.320 kb on - strand at 410.320 kb on - strand at 410.320 kb on - strand at 410.320 kb on - strand at 410.320 kb on - strand at 410.588 kb on + strand, within GFF378 at 410.588 kb on + strand, within GFF378 at 410.588 kb on + strand, within GFF378 at 410.589 kb on - strand, within GFF378 at 410.589 kb on - strand, within GFF378 at 411.175 kb on - strand at 411.316 kb on + strand, within GFF379 at 411.316 kb on + strand, within GFF379 at 411.316 kb on + strand, within GFF379 at 411.317 kb on - strand, within GFF379 at 411.317 kb on - strand, within GFF379 at 411.546 kb on + strand, within GFF379
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 410,196 - -0.3 410,196 - +0.7 410,196 - +0.0 410,300 + +0.9 410,301 - -0.6 410,301 - -0.1 410,301 - +0.2 410,301 - -0.3 410,319 + +0.0 410,319 + -0.1 410,319 + +0.1 410,319 + +0.3 410,319 + +1.4 410,319 + -0.4 410,320 - -0.3 410,320 - +0.1 410,320 - +0.4 410,320 - -0.7 410,320 - -0.5 410,320 - +0.0 410,320 - -0.1 410,320 - -0.7 410,588 + GFF378 0.34 -0.7 410,588 + GFF378 0.34 -0.6 410,588 + GFF378 0.34 -0.9 410,589 - GFF378 0.34 -1.1 410,589 - GFF378 0.34 -2.3 411,175 - +0.0 411,316 + GFF379 0.29 -0.1 411,316 + GFF379 0.29 -0.9 411,316 + GFF379 0.29 -0.4 411,317 - GFF379 0.29 +0.1 411,317 - GFF379 0.29 -0.7 411,546 + GFF379 0.61 -0.5
Or see this region's nucleotide sequence