Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3345 and GFF3346 overlap by 4 nucleotides GFF3346 and GFF3347 are separated by 121 nucleotides GFF3347 and GFF3348 are separated by 4 nucleotides GFF3348 and GFF3349 are separated by 160 nucleotides
GFF3345 - ATP:Cob(I)alamin adenosyltransferase (EC 2.5.1.17) @ ATP:Cob(I)alamin adenosyltransferase (EC 2.5.1.17), glycolate utilization, at 168,956 to 169,555
GFF3345
GFF3346 - hypothetical protein, at 169,552 to 169,914
GFF3346
GFF3347 - Adenylate cyclase (EC 4.6.1.1), at 170,036 to 170,557
GFF3347
GFF3348 - Putative lipase, at 170,562 to 171,203
GFF3348
GFF3349 - Branched-chain amino acid ABC transporter, substrate-binding protein LivJ (TC 3.A.1.4.1), at 171,364 to 172,545
GFF3349
Position (kb) 170
171 Strain fitness (log2 ratio) -2
-1
0
1
2 at 169.406 kb on + strand, within GFF3345 at 169.481 kb on + strand, within GFF3345 at 169.913 kb on - strand at 169.924 kb on - strand at 169.924 kb on - strand at 170.125 kb on - strand, within GFF3347 at 170.223 kb on - strand, within GFF3347 at 170.261 kb on + strand, within GFF3347 at 170.262 kb on - strand, within GFF3347 at 170.262 kb on - strand, within GFF3347 at 170.528 kb on + strand at 170.529 kb on - strand at 170.618 kb on - strand at 170.680 kb on + strand, within GFF3348 at 170.680 kb on + strand, within GFF3348 at 170.681 kb on - strand, within GFF3348 at 170.681 kb on - strand, within GFF3348 at 170.728 kb on + strand, within GFF3348 at 170.729 kb on - strand, within GFF3348 at 170.729 kb on - strand, within GFF3348 at 170.729 kb on - strand, within GFF3348 at 170.729 kb on - strand, within GFF3348 at 170.729 kb on - strand, within GFF3348 at 170.917 kb on + strand, within GFF3348 at 170.918 kb on - strand, within GFF3348 at 171.217 kb on + strand at 171.218 kb on - strand at 171.269 kb on + strand at 171.269 kb on + strand at 171.410 kb on + strand at 171.518 kb on - strand, within GFF3349 at 171.518 kb on - strand, within GFF3349
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 169,406 + GFF3345 0.75 -0.1 169,481 + GFF3345 0.88 +0.0 169,913 - -1.4 169,924 - -0.2 169,924 - +0.6 170,125 - GFF3347 0.17 -1.9 170,223 - GFF3347 0.36 +0.2 170,261 + GFF3347 0.43 -1.6 170,262 - GFF3347 0.43 -1.2 170,262 - GFF3347 0.43 -0.3 170,528 + -0.3 170,529 - +0.2 170,618 - +0.1 170,680 + GFF3348 0.18 +0.3 170,680 + GFF3348 0.18 +1.6 170,681 - GFF3348 0.19 -2.6 170,681 - GFF3348 0.19 -0.3 170,728 + GFF3348 0.26 -0.3 170,729 - GFF3348 0.26 +0.4 170,729 - GFF3348 0.26 -0.1 170,729 - GFF3348 0.26 -0.8 170,729 - GFF3348 0.26 +1.0 170,729 - GFF3348 0.26 -0.5 170,917 + GFF3348 0.55 -1.0 170,918 - GFF3348 0.55 -0.0 171,217 + -0.6 171,218 - -0.4 171,269 + -1.7 171,269 + -1.2 171,410 + +2.2 171,518 - GFF3349 0.13 -1.1 171,518 - GFF3349 0.13 +0.5
Or see this region's nucleotide sequence