Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3214 and GFF3215 overlap by 4 nucleotides GFF3215 and GFF3216 are separated by 188 nucleotides GFF3216 and GFF3217 are separated by 6 nucleotides GFF3217 and GFF3218 are separated by 2 nucleotides
GFF3214 - FIG138928: iron-regulated membrane protein, at 34,423 to 36,090
GFF3214
GFF3215 - no description, at 36,087 to 36,398
GFF3215
GFF3216 - hypothetical protein, at 36,587 to 37,069
GFF3216
GFF3217 - hypothetical protein, at 37,076 to 37,687
GFF3217
GFF3218 - Phage tail fiber, at 37,690 to 38,703
GFF3218
Position (kb)
37
38 Strain fitness (log2 ratio)
-2
-1
0
1 at 36.379 kb on + strand at 36.565 kb on + strand at 36.639 kb on + strand, within GFF3216 at 36.639 kb on + strand, within GFF3216 at 36.640 kb on - strand, within GFF3216 at 36.640 kb on - strand, within GFF3216 at 36.640 kb on - strand, within GFF3216 at 36.640 kb on - strand, within GFF3216 at 36.702 kb on + strand, within GFF3216 at 36.702 kb on + strand, within GFF3216 at 36.702 kb on + strand, within GFF3216 at 36.703 kb on - strand, within GFF3216 at 36.703 kb on - strand, within GFF3216 at 36.703 kb on - strand, within GFF3216 at 36.846 kb on - strand, within GFF3216 at 37.077 kb on + strand at 37.077 kb on + strand at 37.078 kb on - strand at 37.078 kb on - strand at 37.201 kb on - strand, within GFF3217 at 37.284 kb on + strand, within GFF3217 at 37.284 kb on + strand, within GFF3217 at 37.284 kb on + strand, within GFF3217 at 37.285 kb on - strand, within GFF3217 at 37.285 kb on - strand, within GFF3217 at 37.285 kb on - strand, within GFF3217 at 37.318 kb on - strand, within GFF3217 at 37.391 kb on + strand, within GFF3217 at 37.391 kb on + strand, within GFF3217 at 37.391 kb on + strand, within GFF3217 at 37.392 kb on - strand, within GFF3217 at 37.392 kb on - strand, within GFF3217 at 37.392 kb on - strand, within GFF3217 at 37.392 kb on - strand, within GFF3217 at 37.392 kb on - strand, within GFF3217 at 37.392 kb on - strand, within GFF3217 at 37.448 kb on + strand, within GFF3217 at 37.501 kb on + strand, within GFF3217 at 37.501 kb on + strand, within GFF3217 at 37.501 kb on + strand, within GFF3217 at 37.501 kb on + strand, within GFF3217 at 37.501 kb on + strand, within GFF3217 at 37.502 kb on - strand, within GFF3217 at 37.502 kb on - strand, within GFF3217 at 37.502 kb on - strand, within GFF3217 at 37.502 kb on - strand, within GFF3217 at 37.502 kb on - strand, within GFF3217 at 37.502 kb on - strand, within GFF3217 at 37.502 kb on - strand, within GFF3217 at 37.521 kb on + strand, within GFF3217 at 37.521 kb on + strand, within GFF3217 at 37.641 kb on + strand at 37.642 kb on + strand at 37.642 kb on - strand at 37.642 kb on - strand at 37.642 kb on - strand at 37.642 kb on - strand at 37.642 kb on - strand at 37.665 kb on + strand at 37.666 kb on - strand at 37.694 kb on + strand at 37.695 kb on - strand at 37.712 kb on + strand at 37.712 kb on + strand at 37.713 kb on - strand at 37.808 kb on + strand, within GFF3218 at 37.808 kb on + strand, within GFF3218 at 37.808 kb on + strand, within GFF3218 at 37.808 kb on + strand, within GFF3218 at 37.809 kb on - strand, within GFF3218 at 38.018 kb on + strand, within GFF3218 at 38.018 kb on + strand, within GFF3218 at 38.019 kb on - strand, within GFF3218 at 38.019 kb on - strand, within GFF3218 at 38.019 kb on - strand, within GFF3218 at 38.111 kb on + strand, within GFF3218 at 38.111 kb on + strand, within GFF3218 at 38.111 kb on + strand, within GFF3218 at 38.112 kb on - strand, within GFF3218 at 38.125 kb on - strand, within GFF3218 at 38.223 kb on - strand, within GFF3218 at 38.255 kb on + strand, within GFF3218 at 38.255 kb on + strand, within GFF3218 at 38.255 kb on + strand, within GFF3218 at 38.256 kb on - strand, within GFF3218 at 38.256 kb on - strand, within GFF3218 at 38.256 kb on - strand, within GFF3218 at 38.262 kb on + strand, within GFF3218 at 38.324 kb on + strand, within GFF3218 at 38.567 kb on + strand, within GFF3218 at 38.567 kb on + strand, within GFF3218 at 38.567 kb on + strand, within GFF3218 at 38.567 kb on + strand, within GFF3218 at 38.568 kb on - strand, within GFF3218 at 38.616 kb on - strand at 38.638 kb on + strand at 38.639 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 36,379 + +0.2 36,565 + +0.0 36,639 + GFF3216 0.11 +0.1 36,639 + GFF3216 0.11 -0.2 36,640 - GFF3216 0.11 +0.7 36,640 - GFF3216 0.11 -0.7 36,640 - GFF3216 0.11 +1.7 36,640 - GFF3216 0.11 -0.3 36,702 + GFF3216 0.24 +0.7 36,702 + GFF3216 0.24 -0.3 36,702 + GFF3216 0.24 +0.4 36,703 - GFF3216 0.24 -0.8 36,703 - GFF3216 0.24 +0.5 36,703 - GFF3216 0.24 -0.1 36,846 - GFF3216 0.54 +0.4 37,077 + -0.2 37,077 + +0.4 37,078 - +0.5 37,078 - -2.3 37,201 - GFF3217 0.20 -1.8 37,284 + GFF3217 0.34 -0.0 37,284 + GFF3217 0.34 -0.5 37,284 + GFF3217 0.34 -0.3 37,285 - GFF3217 0.34 -1.1 37,285 - GFF3217 0.34 -1.0 37,285 - GFF3217 0.34 -2.3 37,318 - GFF3217 0.40 -0.7 37,391 + GFF3217 0.51 +0.3 37,391 + GFF3217 0.51 -0.2 37,391 + GFF3217 0.51 +0.3 37,392 - GFF3217 0.52 +0.3 37,392 - GFF3217 0.52 -1.6 37,392 - GFF3217 0.52 -0.4 37,392 - GFF3217 0.52 -0.1 37,392 - GFF3217 0.52 -0.3 37,392 - GFF3217 0.52 +0.1 37,448 + GFF3217 0.61 -0.1 37,501 + GFF3217 0.69 -0.1 37,501 + GFF3217 0.69 -0.5 37,501 + GFF3217 0.69 -0.1 37,501 + GFF3217 0.69 -0.8 37,501 + GFF3217 0.69 +0.2 37,502 - GFF3217 0.70 -1.3 37,502 - GFF3217 0.70 -1.0 37,502 - GFF3217 0.70 +0.3 37,502 - GFF3217 0.70 -0.2 37,502 - GFF3217 0.70 -0.1 37,502 - GFF3217 0.70 -0.3 37,502 - GFF3217 0.70 -1.1 37,521 + GFF3217 0.73 -0.9 37,521 + GFF3217 0.73 +0.0 37,641 + +0.3 37,642 + -0.3 37,642 - +0.3 37,642 - -0.8 37,642 - +0.3 37,642 - -0.1 37,642 - -0.5 37,665 + -0.5 37,666 - -0.1 37,694 + -1.0 37,695 - -1.8 37,712 + -0.6 37,712 + +0.0 37,713 - +1.7 37,808 + GFF3218 0.12 +0.5 37,808 + GFF3218 0.12 +0.0 37,808 + GFF3218 0.12 -0.8 37,808 + GFF3218 0.12 -1.6 37,809 - GFF3218 0.12 +1.4 38,018 + GFF3218 0.32 -0.7 38,018 + GFF3218 0.32 +0.2 38,019 - GFF3218 0.32 -0.2 38,019 - GFF3218 0.32 -0.5 38,019 - GFF3218 0.32 +0.4 38,111 + GFF3218 0.42 +0.2 38,111 + GFF3218 0.42 -0.4 38,111 + GFF3218 0.42 +0.7 38,112 - GFF3218 0.42 -0.8 38,125 - GFF3218 0.43 +0.0 38,223 - GFF3218 0.53 +0.5 38,255 + GFF3218 0.56 -1.8 38,255 + GFF3218 0.56 -0.8 38,255 + GFF3218 0.56 -0.3 38,256 - GFF3218 0.56 +0.7 38,256 - GFF3218 0.56 +0.5 38,256 - GFF3218 0.56 -0.4 38,262 + GFF3218 0.56 -0.4 38,324 + GFF3218 0.63 -1.4 38,567 + GFF3218 0.86 -0.1 38,567 + GFF3218 0.86 +0.2 38,567 + GFF3218 0.86 +0.1 38,567 + GFF3218 0.86 +0.4 38,568 - GFF3218 0.87 +1.4 38,616 - +0.2 38,638 + -2.3 38,639 - -0.7
Or see this region's nucleotide sequence