Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2757 and GFF2758 are separated by 15 nucleotides GFF2758 and GFF2759 overlap by 17 nucleotides
GFF2757 - hypothetical protein, at 189,100 to 190,071
GFF2757
GFF2758 - Transcriptional regulator, RpiR family, at 190,087 to 191,040
GFF2758
GFF2759 - hypothetical protein, at 191,024 to 192,070
GFF2759
Position (kb) 190
191
192 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 189.148 kb on - strand at 189.148 kb on - strand at 189.316 kb on + strand, within GFF2757 at 189.316 kb on + strand, within GFF2757 at 189.317 kb on - strand, within GFF2757 at 189.317 kb on - strand, within GFF2757 at 189.317 kb on - strand, within GFF2757 at 189.317 kb on - strand, within GFF2757 at 189.317 kb on - strand, within GFF2757 at 189.607 kb on + strand, within GFF2757 at 189.655 kb on + strand, within GFF2757 at 189.655 kb on + strand, within GFF2757 at 189.656 kb on - strand, within GFF2757 at 189.656 kb on - strand, within GFF2757 at 189.656 kb on - strand, within GFF2757 at 189.656 kb on - strand, within GFF2757 at 189.656 kb on - strand, within GFF2757 at 189.883 kb on + strand, within GFF2757 at 189.883 kb on + strand, within GFF2757 at 189.883 kb on + strand, within GFF2757 at 189.883 kb on + strand, within GFF2757 at 189.884 kb on - strand, within GFF2757 at 189.884 kb on - strand, within GFF2757 at 189.884 kb on - strand, within GFF2757 at 189.884 kb on - strand, within GFF2757 at 189.884 kb on - strand, within GFF2757 at 189.884 kb on - strand, within GFF2757 at 189.884 kb on - strand, within GFF2757 at 189.884 kb on - strand, within GFF2757 at 190.199 kb on + strand, within GFF2758 at 190.199 kb on + strand, within GFF2758 at 190.200 kb on - strand, within GFF2758 at 190.200 kb on - strand, within GFF2758 at 190.358 kb on + strand, within GFF2758 at 190.358 kb on + strand, within GFF2758 at 190.359 kb on - strand, within GFF2758 at 190.478 kb on + strand, within GFF2758 at 190.479 kb on - strand, within GFF2758 at 190.479 kb on - strand, within GFF2758 at 190.727 kb on + strand, within GFF2758 at 190.728 kb on - strand, within GFF2758 at 190.728 kb on - strand, within GFF2758 at 190.794 kb on - strand, within GFF2758 at 191.077 kb on + strand at 191.078 kb on - strand at 191.078 kb on - strand at 191.078 kb on - strand at 191.109 kb on + strand at 191.109 kb on + strand at 191.109 kb on + strand at 191.109 kb on + strand at 191.109 kb on + strand at 191.109 kb on + strand at 191.110 kb on - strand at 191.110 kb on - strand at 191.110 kb on - strand at 191.620 kb on + strand, within GFF2759 at 191.620 kb on + strand, within GFF2759 at 191.620 kb on + strand, within GFF2759 at 191.620 kb on + strand, within GFF2759 at 191.621 kb on - strand, within GFF2759 at 191.621 kb on - strand, within GFF2759 at 191.621 kb on - strand, within GFF2759 at 191.979 kb on + strand at 191.979 kb on + strand at 191.980 kb on - strand at 191.980 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 189,148 - +1.6 189,148 - +0.2 189,316 + GFF2757 0.22 +0.6 189,316 + GFF2757 0.22 -0.2 189,317 - GFF2757 0.22 -0.1 189,317 - GFF2757 0.22 +1.9 189,317 - GFF2757 0.22 +1.1 189,317 - GFF2757 0.22 +0.1 189,317 - GFF2757 0.22 +0.0 189,607 + GFF2757 0.52 -1.1 189,655 + GFF2757 0.57 -0.3 189,655 + GFF2757 0.57 +0.3 189,656 - GFF2757 0.57 -0.5 189,656 - GFF2757 0.57 +0.6 189,656 - GFF2757 0.57 -0.8 189,656 - GFF2757 0.57 -0.1 189,656 - GFF2757 0.57 +0.3 189,883 + GFF2757 0.81 -1.2 189,883 + GFF2757 0.81 -0.6 189,883 + GFF2757 0.81 -1.1 189,883 + GFF2757 0.81 -0.5 189,884 - GFF2757 0.81 -1.1 189,884 - GFF2757 0.81 +0.5 189,884 - GFF2757 0.81 -1.0 189,884 - GFF2757 0.81 -0.7 189,884 - GFF2757 0.81 +0.2 189,884 - GFF2757 0.81 +0.2 189,884 - GFF2757 0.81 +0.2 189,884 - GFF2757 0.81 +0.9 190,199 + GFF2758 0.12 -0.1 190,199 + GFF2758 0.12 +1.1 190,200 - GFF2758 0.12 -0.1 190,200 - GFF2758 0.12 +0.9 190,358 + GFF2758 0.28 -1.3 190,358 + GFF2758 0.28 -2.0 190,359 - GFF2758 0.29 -1.6 190,478 + GFF2758 0.41 +0.4 190,479 - GFF2758 0.41 +1.5 190,479 - GFF2758 0.41 -0.5 190,727 + GFF2758 0.67 -1.2 190,728 - GFF2758 0.67 -1.0 190,728 - GFF2758 0.67 -1.5 190,794 - GFF2758 0.74 +0.1 191,077 + +0.0 191,078 - -1.1 191,078 - -1.0 191,078 - -1.3 191,109 + -1.2 191,109 + -0.8 191,109 + -0.8 191,109 + -0.2 191,109 + -0.0 191,109 + -0.9 191,110 - +0.0 191,110 - +0.0 191,110 - -0.6 191,620 + GFF2759 0.57 -1.0 191,620 + GFF2759 0.57 -0.2 191,620 + GFF2759 0.57 -0.1 191,620 + GFF2759 0.57 -0.9 191,621 - GFF2759 0.57 -0.3 191,621 - GFF2759 0.57 +1.1 191,621 - GFF2759 0.57 -2.9 191,979 + -0.8 191,979 + -0.8 191,980 - -0.1 191,980 - -0.3
Or see this region's nucleotide sequence