Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2600 and GFF2601 are separated by 199 nucleotides GFF2601 and GFF2602 are separated by 8 nucleotides GFF2602 and GFF2603 are separated by 69 nucleotides
GFF2600 - Shikimate 5-dehydrogenase I alpha (EC 1.1.1.25), at 5,672 to 6,595
GFF2600
GFF2601 - Transcriptional regulator, IclR family, at 6,795 to 7,580
GFF2601
GFF2602 - no description, at 7,589 to 8,254
GFF2602
GFF2603 - Tripartite tricarboxylate transporter TctC family, at 8,324 to 9,334
GFF2603
Position (kb)
7
8
9 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 6.647 kb on + strand at 6.647 kb on + strand at 6.648 kb on - strand at 6.648 kb on - strand at 6.648 kb on - strand at 6.678 kb on - strand at 6.759 kb on + strand at 6.760 kb on - strand at 6.760 kb on - strand at 6.760 kb on - strand at 6.760 kb on - strand at 6.990 kb on + strand, within GFF2601 at 6.990 kb on + strand, within GFF2601 at 6.990 kb on + strand, within GFF2601 at 6.990 kb on + strand, within GFF2601 at 6.990 kb on + strand, within GFF2601 at 6.991 kb on - strand, within GFF2601 at 6.991 kb on - strand, within GFF2601 at 6.991 kb on - strand, within GFF2601 at 7.053 kb on + strand, within GFF2601 at 7.054 kb on - strand, within GFF2601 at 7.054 kb on - strand, within GFF2601 at 7.054 kb on - strand, within GFF2601 at 7.132 kb on - strand, within GFF2601 at 7.132 kb on - strand, within GFF2601 at 7.132 kb on - strand, within GFF2601 at 7.164 kb on + strand, within GFF2601 at 7.164 kb on + strand, within GFF2601 at 7.164 kb on + strand, within GFF2601 at 7.165 kb on - strand, within GFF2601 at 7.165 kb on - strand, within GFF2601 at 7.165 kb on - strand, within GFF2601 at 7.165 kb on - strand, within GFF2601 at 7.165 kb on - strand, within GFF2601 at 7.206 kb on + strand, within GFF2601 at 7.206 kb on + strand, within GFF2601 at 7.206 kb on + strand, within GFF2601 at 7.207 kb on - strand, within GFF2601 at 7.207 kb on - strand, within GFF2601 at 7.207 kb on - strand, within GFF2601 at 7.207 kb on - strand, within GFF2601 at 7.238 kb on - strand, within GFF2601 at 7.706 kb on + strand, within GFF2602 at 7.707 kb on - strand, within GFF2602 at 7.707 kb on - strand, within GFF2602 at 7.709 kb on + strand, within GFF2602 at 7.766 kb on + strand, within GFF2602 at 7.779 kb on + strand, within GFF2602 at 8.042 kb on + strand, within GFF2602 at 8.043 kb on - strand, within GFF2602 at 8.090 kb on + strand, within GFF2602 at 8.090 kb on + strand, within GFF2602 at 8.090 kb on + strand, within GFF2602 at 8.090 kb on + strand, within GFF2602 at 8.090 kb on + strand, within GFF2602 at 8.090 kb on + strand, within GFF2602 at 8.090 kb on + strand, within GFF2602 at 8.090 kb on + strand, within GFF2602 at 8.091 kb on - strand, within GFF2602 at 8.091 kb on - strand, within GFF2602 at 8.091 kb on - strand, within GFF2602 at 8.091 kb on - strand, within GFF2602 at 8.091 kb on - strand, within GFF2602 at 8.091 kb on - strand, within GFF2602 at 8.115 kb on + strand, within GFF2602 at 8.147 kb on + strand, within GFF2602 at 8.234 kb on + strand at 8.234 kb on + strand at 8.235 kb on - strand at 8.666 kb on + strand, within GFF2603 at 8.666 kb on + strand, within GFF2603 at 8.667 kb on - strand, within GFF2603 at 8.667 kb on - strand, within GFF2603 at 8.667 kb on - strand, within GFF2603 at 8.744 kb on + strand, within GFF2603 at 8.744 kb on + strand, within GFF2603 at 8.744 kb on + strand, within GFF2603 at 8.744 kb on + strand, within GFF2603 at 8.744 kb on + strand, within GFF2603 at 8.745 kb on - strand, within GFF2603 at 8.745 kb on - strand, within GFF2603 at 8.745 kb on - strand, within GFF2603 at 8.795 kb on + strand, within GFF2603 at 8.825 kb on + strand, within GFF2603 at 8.825 kb on + strand, within GFF2603 at 8.826 kb on - strand, within GFF2603 at 8.826 kb on - strand, within GFF2603 at 8.826 kb on - strand, within GFF2603 at 8.870 kb on + strand, within GFF2603 at 8.871 kb on - strand, within GFF2603 at 9.116 kb on + strand, within GFF2603 at 9.117 kb on - strand, within GFF2603
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 6,647 + -0.4 6,647 + +0.2 6,648 - +0.1 6,648 - -0.4 6,648 - -1.2 6,678 - -1.7 6,759 + +0.5 6,760 - -0.2 6,760 - -0.4 6,760 - +0.1 6,760 - -1.0 6,990 + GFF2601 0.25 -0.7 6,990 + GFF2601 0.25 +1.1 6,990 + GFF2601 0.25 -1.0 6,990 + GFF2601 0.25 +0.7 6,990 + GFF2601 0.25 +0.9 6,991 - GFF2601 0.25 -0.1 6,991 - GFF2601 0.25 +0.8 6,991 - GFF2601 0.25 +0.9 7,053 + GFF2601 0.33 +0.0 7,054 - GFF2601 0.33 -0.5 7,054 - GFF2601 0.33 +0.1 7,054 - GFF2601 0.33 +0.0 7,132 - GFF2601 0.43 -1.2 7,132 - GFF2601 0.43 -1.8 7,132 - GFF2601 0.43 -1.3 7,164 + GFF2601 0.47 -0.8 7,164 + GFF2601 0.47 +1.2 7,164 + GFF2601 0.47 -1.3 7,165 - GFF2601 0.47 -0.8 7,165 - GFF2601 0.47 -0.1 7,165 - GFF2601 0.47 +0.5 7,165 - GFF2601 0.47 -0.0 7,165 - GFF2601 0.47 -0.3 7,206 + GFF2601 0.52 -0.5 7,206 + GFF2601 0.52 +0.0 7,206 + GFF2601 0.52 -0.3 7,207 - GFF2601 0.52 -0.9 7,207 - GFF2601 0.52 -1.2 7,207 - GFF2601 0.52 +0.4 7,207 - GFF2601 0.52 +0.5 7,238 - GFF2601 0.56 +0.8 7,706 + GFF2602 0.18 -0.5 7,707 - GFF2602 0.18 -0.2 7,707 - GFF2602 0.18 -0.8 7,709 + GFF2602 0.18 +0.1 7,766 + GFF2602 0.27 -0.7 7,779 + GFF2602 0.29 +0.6 8,042 + GFF2602 0.68 -0.2 8,043 - GFF2602 0.68 +0.7 8,090 + GFF2602 0.75 -0.8 8,090 + GFF2602 0.75 -1.0 8,090 + GFF2602 0.75 -0.7 8,090 + GFF2602 0.75 +1.5 8,090 + GFF2602 0.75 -0.9 8,090 + GFF2602 0.75 -0.7 8,090 + GFF2602 0.75 -0.5 8,090 + GFF2602 0.75 -1.3 8,091 - GFF2602 0.75 -0.3 8,091 - GFF2602 0.75 +0.1 8,091 - GFF2602 0.75 -0.2 8,091 - GFF2602 0.75 -0.8 8,091 - GFF2602 0.75 +0.2 8,091 - GFF2602 0.75 -0.7 8,115 + GFF2602 0.79 -0.3 8,147 + GFF2602 0.84 +1.2 8,234 + -0.2 8,234 + -0.6 8,235 - -0.6 8,666 + GFF2603 0.34 -0.1 8,666 + GFF2603 0.34 +0.8 8,667 - GFF2603 0.34 -0.5 8,667 - GFF2603 0.34 +0.1 8,667 - GFF2603 0.34 -0.1 8,744 + GFF2603 0.42 +0.2 8,744 + GFF2603 0.42 +0.2 8,744 + GFF2603 0.42 +2.1 8,744 + GFF2603 0.42 -0.2 8,744 + GFF2603 0.42 +0.5 8,745 - GFF2603 0.42 +1.5 8,745 - GFF2603 0.42 -1.1 8,745 - GFF2603 0.42 +0.8 8,795 + GFF2603 0.47 +0.7 8,825 + GFF2603 0.50 +0.7 8,825 + GFF2603 0.50 +0.3 8,826 - GFF2603 0.50 +0.1 8,826 - GFF2603 0.50 -0.3 8,826 - GFF2603 0.50 -0.2 8,870 + GFF2603 0.54 -1.1 8,871 - GFF2603 0.54 +0.2 9,116 + GFF2603 0.78 -0.1 9,117 - GFF2603 0.78 -0.7
Or see this region's nucleotide sequence