Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2087 and GFF2088 overlap by 4 nucleotides GFF2088 and GFF2089 are separated by 27 nucleotides GFF2089 and GFF2090 are separated by 85 nucleotides
GFF2087 - Ser/Thr and Tyr protein phosphatase (dual specificity), at 134,074 to 135,423
GFF2087
GFF2088 - Putative lipase in cluster with Phosphatidate cytidylyltransferase, at 135,420 to 137,186
GFF2088
GFF2089 - CDP-alcohol phosphatidyltransferase family protein, at 137,214 to 137,837
GFF2089
GFF2090 - Holliday junction ATP-dependent DNA helicase RuvB (EC 3.6.4.12), at 137,923 to 138,987
GFF2090
Position (kb)
135
136
137
138 Strain fitness (log2 ratio)
-2
-1
0
1 at 134.642 kb on + strand, within GFF2087 at 134.642 kb on + strand, within GFF2087 at 134.642 kb on + strand, within GFF2087 at 134.642 kb on + strand, within GFF2087 at 134.643 kb on - strand, within GFF2087 at 134.747 kb on + strand, within GFF2087 at 134.748 kb on - strand, within GFF2087 at 134.785 kb on - strand, within GFF2087 at 135.006 kb on + strand, within GFF2087 at 135.006 kb on + strand, within GFF2087 at 135.007 kb on - strand, within GFF2087 at 135.218 kb on + strand, within GFF2087 at 135.218 kb on + strand, within GFF2087 at 135.219 kb on - strand, within GFF2087 at 135.240 kb on - strand, within GFF2087 at 135.268 kb on + strand, within GFF2087 at 135.269 kb on - strand, within GFF2087 at 135.341 kb on + strand at 135.341 kb on + strand at 135.341 kb on + strand at 135.350 kb on + strand at 135.350 kb on + strand at 135.350 kb on + strand at 135.350 kb on + strand at 135.351 kb on - strand at 135.351 kb on - strand at 135.351 kb on - strand at 135.351 kb on - strand at 135.351 kb on - strand at 135.625 kb on + strand, within GFF2088 at 135.626 kb on - strand, within GFF2088 at 135.710 kb on - strand, within GFF2088 at 135.920 kb on - strand, within GFF2088 at 135.920 kb on - strand, within GFF2088 at 135.999 kb on + strand, within GFF2088 at 135.999 kb on + strand, within GFF2088 at 135.999 kb on + strand, within GFF2088 at 136.000 kb on - strand, within GFF2088 at 136.057 kb on + strand, within GFF2088 at 136.058 kb on - strand, within GFF2088 at 136.111 kb on + strand, within GFF2088 at 136.117 kb on + strand, within GFF2088 at 136.117 kb on + strand, within GFF2088 at 136.117 kb on + strand, within GFF2088 at 136.219 kb on + strand, within GFF2088 at 136.624 kb on + strand, within GFF2088 at 136.624 kb on + strand, within GFF2088 at 136.625 kb on - strand, within GFF2088 at 136.625 kb on - strand, within GFF2088 at 136.672 kb on + strand, within GFF2088 at 136.673 kb on - strand, within GFF2088 at 136.673 kb on - strand, within GFF2088 at 136.741 kb on + strand, within GFF2088 at 136.742 kb on - strand, within GFF2088 at 136.742 kb on - strand, within GFF2088 at 136.742 kb on - strand, within GFF2088 at 136.798 kb on + strand, within GFF2088 at 136.798 kb on + strand, within GFF2088 at 137.116 kb on + strand at 137.116 kb on + strand at 137.117 kb on - strand at 137.210 kb on + strand at 137.211 kb on - strand at 137.211 kb on - strand at 137.211 kb on - strand at 137.276 kb on + strand at 137.353 kb on + strand, within GFF2089 at 137.353 kb on + strand, within GFF2089 at 137.353 kb on + strand, within GFF2089 at 137.354 kb on - strand, within GFF2089 at 137.354 kb on - strand, within GFF2089 at 137.354 kb on - strand, within GFF2089 at 137.354 kb on - strand, within GFF2089 at 137.531 kb on - strand, within GFF2089 at 137.531 kb on - strand, within GFF2089 at 137.827 kb on + strand at 137.861 kb on - strand at 137.861 kb on - strand at 137.892 kb on + strand at 137.892 kb on + strand at 137.893 kb on - strand at 137.895 kb on + strand at 137.895 kb on + strand at 137.895 kb on + strand at 137.903 kb on + strand at 137.928 kb on + strand at 138.040 kb on + strand, within GFF2090 at 138.041 kb on - strand, within GFF2090
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 134,642 + GFF2087 0.42 -0.2 134,642 + GFF2087 0.42 -0.4 134,642 + GFF2087 0.42 -2.0 134,642 + GFF2087 0.42 -0.1 134,643 - GFF2087 0.42 +0.1 134,747 + GFF2087 0.50 -0.3 134,748 - GFF2087 0.50 +0.5 134,785 - GFF2087 0.53 -0.9 135,006 + GFF2087 0.69 +0.3 135,006 + GFF2087 0.69 -1.1 135,007 - GFF2087 0.69 -0.1 135,218 + GFF2087 0.85 -0.3 135,218 + GFF2087 0.85 +0.2 135,219 - GFF2087 0.85 -0.3 135,240 - GFF2087 0.86 +0.7 135,268 + GFF2087 0.88 +0.5 135,269 - GFF2087 0.89 -0.4 135,341 + -0.4 135,341 + +0.8 135,341 + +1.2 135,350 + -0.0 135,350 + -1.1 135,350 + +0.1 135,350 + +0.1 135,351 - +0.1 135,351 - -0.1 135,351 - -0.4 135,351 - +0.8 135,351 - +0.0 135,625 + GFF2088 0.12 +1.3 135,626 - GFF2088 0.12 -0.1 135,710 - GFF2088 0.16 -0.3 135,920 - GFF2088 0.28 +0.2 135,920 - GFF2088 0.28 +0.2 135,999 + GFF2088 0.33 -0.3 135,999 + GFF2088 0.33 +0.4 135,999 + GFF2088 0.33 -0.8 136,000 - GFF2088 0.33 +0.9 136,057 + GFF2088 0.36 +1.1 136,058 - GFF2088 0.36 -0.8 136,111 + GFF2088 0.39 +1.1 136,117 + GFF2088 0.39 +0.1 136,117 + GFF2088 0.39 +0.0 136,117 + GFF2088 0.39 -0.1 136,219 + GFF2088 0.45 +0.0 136,624 + GFF2088 0.68 +0.1 136,624 + GFF2088 0.68 +1.0 136,625 - GFF2088 0.68 -0.9 136,625 - GFF2088 0.68 -0.0 136,672 + GFF2088 0.71 -0.3 136,673 - GFF2088 0.71 -0.5 136,673 - GFF2088 0.71 +1.1 136,741 + GFF2088 0.75 -0.5 136,742 - GFF2088 0.75 +0.2 136,742 - GFF2088 0.75 +0.3 136,742 - GFF2088 0.75 -0.3 136,798 + GFF2088 0.78 -0.5 136,798 + GFF2088 0.78 +0.0 137,116 + +0.1 137,116 + +0.3 137,117 - +0.5 137,210 + -0.6 137,211 - -0.6 137,211 - -0.2 137,211 - +0.2 137,276 + +0.1 137,353 + GFF2089 0.22 +0.3 137,353 + GFF2089 0.22 +0.0 137,353 + GFF2089 0.22 +0.1 137,354 - GFF2089 0.22 +0.2 137,354 - GFF2089 0.22 -1.5 137,354 - GFF2089 0.22 -0.2 137,354 - GFF2089 0.22 -0.3 137,531 - GFF2089 0.51 +0.4 137,531 - GFF2089 0.51 -0.6 137,827 + -0.9 137,861 - +0.0 137,861 - -0.6 137,892 + -0.1 137,892 + -0.8 137,893 - -1.5 137,895 + -0.6 137,895 + -1.5 137,895 + -0.2 137,903 + +0.1 137,928 + +0.1 138,040 + GFF2090 0.11 -0.5 138,041 - GFF2090 0.11 +0.1
Or see this region's nucleotide sequence