Strain Fitness in Variovorax sp. SCN45 around GFF1987

Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1985 and GFF1986 are separated by 21 nucleotidesGFF1986 and GFF1987 are separated by 68 nucleotidesGFF1987 and GFF1988 overlap by 4 nucleotides GFF1985 - Zinc metalloprotease, at 26,774 to 27,754 GFF1985 GFF1986 - Oxidoreductase, short-chain dehydrogenase/reductase family, at 27,776 to 28,546 GFF1986 GFF1987 - Pseudouridine synthase (EC 4.2.1.70), PA2043 type, at 28,615 to 29,535 GFF1987 GFF1988 - Serine/threonine protein kinase, at 29,532 to 30,842 GFF1988 Position (kb) 28 29 30Strain fitness (log2 ratio) -2 -1 0 1 2at 27.615 kb on - strand, within GFF1985at 27.615 kb on - strand, within GFF1985at 27.701 kb on + strandat 27.701 kb on + strandat 27.828 kb on + strandat 27.828 kb on + strandat 27.829 kb on - strandat 27.829 kb on - strandat 27.829 kb on - strandat 27.829 kb on - strandat 27.852 kb on + strandat 27.852 kb on + strandat 27.852 kb on + strandat 27.852 kb on + strandat 27.852 kb on + strandat 27.852 kb on + strandat 27.852 kb on + strandat 27.852 kb on + strandat 27.853 kb on - strand, within GFF1986at 27.853 kb on - strand, within GFF1986at 27.853 kb on - strand, within GFF1986at 28.059 kb on + strand, within GFF1986at 28.065 kb on + strand, within GFF1986at 28.066 kb on - strand, within GFF1986at 28.066 kb on - strand, within GFF1986at 28.066 kb on - strand, within GFF1986at 28.305 kb on + strand, within GFF1986at 28.306 kb on - strand, within GFF1986at 28.431 kb on + strand, within GFF1986at 28.432 kb on - strand, within GFF1986at 28.432 kb on - strand, within GFF1986at 28.432 kb on - strand, within GFF1986at 28.590 kb on + strandat 28.590 kb on + strandat 28.757 kb on + strand, within GFF1987at 28.758 kb on - strand, within GFF1987at 28.758 kb on - strand, within GFF1987at 28.958 kb on + strand, within GFF1987at 28.958 kb on + strand, within GFF1987at 28.958 kb on + strand, within GFF1987at 28.958 kb on + strand, within GFF1987at 28.959 kb on - strand, within GFF1987at 29.009 kb on + strand, within GFF1987at 29.009 kb on + strand, within GFF1987at 29.010 kb on - strand, within GFF1987at 29.010 kb on - strand, within GFF1987at 29.010 kb on - strand, within GFF1987at 29.010 kb on - strand, within GFF1987at 29.048 kb on + strand, within GFF1987at 29.180 kb on + strand, within GFF1987at 29.180 kb on + strand, within GFF1987at 29.180 kb on + strand, within GFF1987at 29.180 kb on + strand, within GFF1987at 29.180 kb on + strand, within GFF1987at 29.181 kb on - strand, within GFF1987at 29.181 kb on - strand, within GFF1987at 29.181 kb on - strand, within GFF1987at 29.181 kb on - strand, within GFF1987at 29.181 kb on - strand, within GFF1987at 29.181 kb on - strand, within GFF1987at 29.297 kb on + strand, within GFF1987at 29.297 kb on + strand, within GFF1987at 29.300 kb on + strand, within GFF1987at 29.300 kb on + strand, within GFF1987at 29.300 kb on + strand, within GFF1987at 29.300 kb on + strand, within GFF1987at 29.300 kb on + strand, within GFF1987at 29.300 kb on + strand, within GFF1987at 29.301 kb on - strand, within GFF1987at 29.301 kb on - strand, within GFF1987at 29.301 kb on - strand, within GFF1987at 29.301 kb on - strand, within GFF1987at 29.301 kb on - strand, within GFF1987at 29.301 kb on - strand, within GFF1987at 29.301 kb on - strand, within GFF1987at 29.301 kb on - strand, within GFF1987at 29.301 kb on - strand, within GFF1987at 29.301 kb on - strand, within GFF1987at 29.301 kb on - strand, within GFF1987at 29.302 kb on + strand, within GFF1987at 29.303 kb on + strand, within GFF1987at 29.303 kb on + strand, within GFF1987at 29.303 kb on + strand, within GFF1987at 29.303 kb on + strand, within GFF1987at 29.303 kb on + strand, within GFF1987at 29.303 kb on + strand, within GFF1987at 29.304 kb on - strand, within GFF1987at 29.304 kb on - strand, within GFF1987at 29.304 kb on - strand, within GFF1987at 29.304 kb on - strand, within GFF1987at 29.304 kb on - strand, within GFF1987at 29.304 kb on - strand, within GFF1987at 29.325 kb on - strand, within GFF1987at 29.553 kb on + strandat 29.553 kb on + strandat 29.554 kb on - strandat 29.554 kb on - strandat 29.554 kb on - strandat 29.554 kb on - strandat 29.812 kb on + strand, within GFF1988at 29.812 kb on + strand, within GFF1988at 29.812 kb on + strand, within GFF1988at 29.813 kb on - strand, within GFF1988at 29.813 kb on - strand, within GFF1988at 29.813 kb on - strand, within GFF1988at 29.813 kb on - strand, within GFF1988at 29.920 kb on + strand, within GFF1988at 29.921 kb on - strand, within GFF1988at 29.921 kb on - strand, within GFF1988

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
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27,615 - GFF1985 0.86 +1.3
27,615 - GFF1985 0.86 -0.4
27,701 + -0.2
27,701 + -0.6
27,828 + +0.7
27,828 + -0.3
27,829 - +0.2
27,829 - +0.2
27,829 - +0.7
27,829 - -1.0
27,852 + +0.7
27,852 + +0.1
27,852 + +0.9
27,852 + +0.5
27,852 + -0.2
27,852 + -0.0
27,852 + +0.4
27,852 + +0.8
27,853 - GFF1986 0.10 +0.2
27,853 - GFF1986 0.10 -0.8
27,853 - GFF1986 0.10 -0.4
28,059 + GFF1986 0.37 +0.2
28,065 + GFF1986 0.37 -0.6
28,066 - GFF1986 0.38 -0.4
28,066 - GFF1986 0.38 -0.2
28,066 - GFF1986 0.38 +0.3
28,305 + GFF1986 0.69 -1.6
28,306 - GFF1986 0.69 -0.3
28,431 + GFF1986 0.85 -0.7
28,432 - GFF1986 0.85 -0.3
28,432 - GFF1986 0.85 -1.7
28,432 - GFF1986 0.85 -1.6
28,590 + -0.5
28,590 + -1.0
28,757 + GFF1987 0.15 +0.1
28,758 - GFF1987 0.16 +0.1
28,758 - GFF1987 0.16 -0.6
28,958 + GFF1987 0.37 -1.2
28,958 + GFF1987 0.37 -0.1
28,958 + GFF1987 0.37 -0.5
28,958 + GFF1987 0.37 -1.8
28,959 - GFF1987 0.37 -0.4
29,009 + GFF1987 0.43 -0.4
29,009 + GFF1987 0.43 -0.6
29,010 - GFF1987 0.43 -2.4
29,010 - GFF1987 0.43 +1.3
29,010 - GFF1987 0.43 -0.5
29,010 - GFF1987 0.43 -0.6
29,048 + GFF1987 0.47 -0.6
29,180 + GFF1987 0.61 -0.1
29,180 + GFF1987 0.61 -0.0
29,180 + GFF1987 0.61 -0.6
29,180 + GFF1987 0.61 -2.3
29,180 + GFF1987 0.61 -0.9
29,181 - GFF1987 0.61 -1.1
29,181 - GFF1987 0.61 -1.3
29,181 - GFF1987 0.61 -1.1
29,181 - GFF1987 0.61 +0.1
29,181 - GFF1987 0.61 +0.1
29,181 - GFF1987 0.61 +0.4
29,297 + GFF1987 0.74 +1.7
29,297 + GFF1987 0.74 -1.1
29,300 + GFF1987 0.74 +0.7
29,300 + GFF1987 0.74 -0.2
29,300 + GFF1987 0.74 +1.8
29,300 + GFF1987 0.74 +1.0
29,300 + GFF1987 0.74 -0.4
29,300 + GFF1987 0.74 +0.5
29,301 - GFF1987 0.74 -1.1
29,301 - GFF1987 0.74 -1.0
29,301 - GFF1987 0.74 -0.1
29,301 - GFF1987 0.74 -1.6
29,301 - GFF1987 0.74 -0.0
29,301 - GFF1987 0.74 -0.6
29,301 - GFF1987 0.74 -0.7
29,301 - GFF1987 0.74 +0.4
29,301 - GFF1987 0.74 -0.5
29,301 - GFF1987 0.74 -1.3
29,301 - GFF1987 0.74 -0.6
29,302 + GFF1987 0.75 +0.2
29,303 + GFF1987 0.75 +0.3
29,303 + GFF1987 0.75 -0.2
29,303 + GFF1987 0.75 -0.6
29,303 + GFF1987 0.75 -0.6
29,303 + GFF1987 0.75 +0.7
29,303 + GFF1987 0.75 -1.9
29,304 - GFF1987 0.75 -0.1
29,304 - GFF1987 0.75 -0.1
29,304 - GFF1987 0.75 -0.6
29,304 - GFF1987 0.75 -0.3
29,304 - GFF1987 0.75 -0.7
29,304 - GFF1987 0.75 -1.3
29,325 - GFF1987 0.77 +1.0
29,553 + -1.3
29,553 + -0.5
29,554 - -1.0
29,554 - +0.2
29,554 - -2.4
29,554 - +1.1
29,812 + GFF1988 0.21 +0.2
29,812 + GFF1988 0.21 -0.3
29,812 + GFF1988 0.21 -0.3
29,813 - GFF1988 0.21 -0.1
29,813 - GFF1988 0.21 +0.1
29,813 - GFF1988 0.21 -1.9
29,813 - GFF1988 0.21 -1.1
29,920 + GFF1988 0.30 -1.0
29,921 - GFF1988 0.30 -0.4
29,921 - GFF1988 0.30 +0.3

Or see this region's nucleotide sequence