Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1823 and GFF1824 are separated by 37 nucleotides GFF1824 and GFF1825 are separated by 84 nucleotides GFF1825 and GFF1826 are separated by 207 nucleotides GFF1826 and GFF1827 are separated by 464 nucleotides
GFF1823 - Mg(2+)-transport-ATPase-associated protein MgtC, at 487,708 to 488,424
GFF1823
GFF1824 - hypothetical protein, at 488,462 to 489,037
GFF1824
GFF1825 - Mg(2+) transport ATPase, P-type (EC 3.6.3.2), at 489,122 to 491,887
GFF1825
GFF1826 - hypothetical protein, at 492,095 to 492,298
GFF1826
GFF1827 - Low molecular weight protein tyrosine phosphatase (EC 3.1.3.48), at 492,763 to 493,170
GFF1827
Position (kb) 489
490
491
492 Strain fitness (log2 ratio) -2
-1
0
1
2 at 488.130 kb on + strand, within GFF1823 at 488.131 kb on - strand, within GFF1823 at 488.204 kb on + strand, within GFF1823 at 488.204 kb on + strand, within GFF1823 at 488.205 kb on - strand, within GFF1823 at 488.205 kb on - strand, within GFF1823 at 488.205 kb on - strand, within GFF1823 at 488.205 kb on - strand, within GFF1823 at 488.205 kb on - strand, within GFF1823 at 488.205 kb on - strand, within GFF1823 at 488.447 kb on + strand at 488.447 kb on + strand at 488.448 kb on - strand at 488.565 kb on + strand, within GFF1824 at 488.566 kb on - strand, within GFF1824 at 488.637 kb on + strand, within GFF1824 at 488.637 kb on + strand, within GFF1824 at 488.637 kb on + strand, within GFF1824 at 488.638 kb on - strand, within GFF1824 at 488.638 kb on - strand, within GFF1824 at 488.838 kb on + strand, within GFF1824 at 488.838 kb on + strand, within GFF1824 at 488.838 kb on + strand, within GFF1824 at 488.838 kb on + strand, within GFF1824 at 488.838 kb on + strand, within GFF1824 at 488.839 kb on - strand, within GFF1824 at 488.839 kb on - strand, within GFF1824 at 488.839 kb on - strand, within GFF1824 at 488.918 kb on + strand, within GFF1824 at 488.918 kb on + strand, within GFF1824 at 488.919 kb on - strand, within GFF1824 at 489.039 kb on + strand at 489.039 kb on + strand at 489.039 kb on + strand at 489.039 kb on + strand at 489.039 kb on + strand at 489.039 kb on + strand at 489.039 kb on + strand at 489.039 kb on + strand at 489.040 kb on - strand at 489.040 kb on - strand at 489.040 kb on - strand at 489.040 kb on - strand at 489.069 kb on + strand at 489.070 kb on - strand at 489.070 kb on - strand at 489.116 kb on + strand at 489.117 kb on - strand at 489.117 kb on - strand at 489.117 kb on - strand at 489.180 kb on + strand at 489.214 kb on - strand at 489.214 kb on - strand at 489.214 kb on - strand at 489.214 kb on - strand at 489.214 kb on - strand at 489.214 kb on - strand at 489.243 kb on + strand at 489.243 kb on + strand at 489.609 kb on + strand, within GFF1825 at 489.727 kb on - strand, within GFF1825 at 489.727 kb on - strand, within GFF1825 at 489.727 kb on - strand, within GFF1825 at 489.727 kb on - strand, within GFF1825 at 489.827 kb on + strand, within GFF1825 at 489.828 kb on - strand, within GFF1825 at 489.828 kb on - strand, within GFF1825 at 489.828 kb on - strand, within GFF1825 at 490.200 kb on + strand, within GFF1825 at 490.201 kb on - strand, within GFF1825 at 490.201 kb on - strand, within GFF1825 at 490.201 kb on - strand, within GFF1825 at 490.239 kb on + strand, within GFF1825 at 490.240 kb on - strand, within GFF1825 at 490.240 kb on - strand, within GFF1825 at 490.240 kb on - strand, within GFF1825 at 490.240 kb on - strand, within GFF1825 at 490.515 kb on + strand, within GFF1825 at 490.614 kb on + strand, within GFF1825 at 491.529 kb on + strand, within GFF1825 at 491.530 kb on - strand, within GFF1825 at 491.530 kb on - strand, within GFF1825 at 491.577 kb on + strand, within GFF1825 at 492.463 kb on - strand at 492.606 kb on + strand at 492.606 kb on + strand at 492.625 kb on + strand at 492.712 kb on + strand at 492.712 kb on + strand at 492.712 kb on + strand at 492.712 kb on + strand at 492.713 kb on - strand at 492.713 kb on - strand at 492.713 kb on - strand at 492.714 kb on + strand at 492.715 kb on - strand at 492.715 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 488,130 + GFF1823 0.59 +0.2 488,131 - GFF1823 0.59 -2.1 488,204 + GFF1823 0.69 -1.0 488,204 + GFF1823 0.69 -1.1 488,205 - GFF1823 0.69 +0.7 488,205 - GFF1823 0.69 -0.0 488,205 - GFF1823 0.69 -0.3 488,205 - GFF1823 0.69 -0.7 488,205 - GFF1823 0.69 +0.6 488,205 - GFF1823 0.69 +0.2 488,447 + -0.1 488,447 + -0.9 488,448 - -0.5 488,565 + GFF1824 0.18 -0.2 488,566 - GFF1824 0.18 -0.6 488,637 + GFF1824 0.30 +0.3 488,637 + GFF1824 0.30 -0.8 488,637 + GFF1824 0.30 -1.7 488,638 - GFF1824 0.31 -1.0 488,638 - GFF1824 0.31 +0.8 488,838 + GFF1824 0.65 +1.1 488,838 + GFF1824 0.65 -0.2 488,838 + GFF1824 0.65 +1.4 488,838 + GFF1824 0.65 -0.7 488,838 + GFF1824 0.65 +0.2 488,839 - GFF1824 0.65 +0.7 488,839 - GFF1824 0.65 -0.4 488,839 - GFF1824 0.65 +2.1 488,918 + GFF1824 0.79 +1.1 488,918 + GFF1824 0.79 -1.1 488,919 - GFF1824 0.79 -0.2 489,039 + -0.4 489,039 + -0.3 489,039 + -0.1 489,039 + +0.4 489,039 + +0.2 489,039 + -0.4 489,039 + -1.0 489,039 + -0.5 489,040 - +0.3 489,040 - +0.1 489,040 - -0.2 489,040 - +0.1 489,069 + +0.1 489,070 - -0.7 489,070 - -0.2 489,116 + +0.1 489,117 - -0.1 489,117 - -0.6 489,117 - -0.7 489,180 + -1.1 489,214 - +0.9 489,214 - -0.3 489,214 - +0.3 489,214 - -0.1 489,214 - +0.2 489,214 - -0.6 489,243 + -0.1 489,243 + +0.3 489,609 + GFF1825 0.18 -1.3 489,727 - GFF1825 0.22 -1.2 489,727 - GFF1825 0.22 -0.3 489,727 - GFF1825 0.22 +0.2 489,727 - GFF1825 0.22 -0.8 489,827 + GFF1825 0.25 -1.1 489,828 - GFF1825 0.26 -0.1 489,828 - GFF1825 0.26 -0.2 489,828 - GFF1825 0.26 -0.1 490,200 + GFF1825 0.39 +0.0 490,201 - GFF1825 0.39 +0.2 490,201 - GFF1825 0.39 -1.2 490,201 - GFF1825 0.39 -0.9 490,239 + GFF1825 0.40 -0.1 490,240 - GFF1825 0.40 -0.2 490,240 - GFF1825 0.40 -0.1 490,240 - GFF1825 0.40 -1.4 490,240 - GFF1825 0.40 -0.2 490,515 + GFF1825 0.50 -0.8 490,614 + GFF1825 0.54 +0.4 491,529 + GFF1825 0.87 -0.6 491,530 - GFF1825 0.87 -0.4 491,530 - GFF1825 0.87 -1.9 491,577 + GFF1825 0.89 +0.0 492,463 - -0.4 492,606 + -0.3 492,606 + -0.7 492,625 + +1.3 492,712 + +0.5 492,712 + -0.8 492,712 + -0.7 492,712 + -0.7 492,713 - -0.7 492,713 - +0.1 492,713 - -2.0 492,714 + -1.5 492,715 - +0.1 492,715 - +1.9
Or see this region's nucleotide sequence