Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1736 and GFF1737 are separated by 212 nucleotides GFF1737 and GFF1738 are separated by 4 nucleotides GFF1738 and GFF1739 are separated by 128 nucleotides
GFF1736 - 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases, at 394,721 to 396,010
GFF1736
GFF1737 - Carbon starvation protein A, at 396,223 to 398,289
GFF1737
GFF1738 - Uncharacterized proteins YbdD and YjiX, at 398,294 to 398,500
GFF1738
GFF1739 - Glutamate carboxypeptidase (EC 3.4.17.11), at 398,629 to 399,906
GFF1739
Position (kb) 396
397
398
399 Strain fitness (log2 ratio) -2
-1
0
1 at 395.304 kb on + strand, within GFF1736 at 395.305 kb on - strand, within GFF1736 at 395.305 kb on - strand, within GFF1736 at 395.421 kb on + strand, within GFF1736 at 395.422 kb on - strand, within GFF1736 at 395.810 kb on + strand, within GFF1736 at 396.042 kb on + strand at 396.042 kb on + strand at 396.043 kb on - strand at 396.043 kb on - strand at 396.043 kb on - strand at 396.043 kb on - strand at 396.073 kb on + strand at 396.073 kb on + strand at 396.355 kb on + strand at 396.355 kb on + strand at 396.355 kb on + strand at 396.355 kb on + strand at 396.355 kb on + strand at 396.356 kb on - strand at 396.356 kb on - strand at 396.368 kb on - strand at 396.373 kb on + strand at 396.373 kb on + strand at 396.376 kb on + strand at 396.376 kb on + strand at 396.376 kb on + strand at 396.376 kb on + strand at 396.376 kb on + strand at 396.377 kb on - strand at 396.461 kb on - strand, within GFF1737 at 396.559 kb on + strand, within GFF1737 at 396.560 kb on - strand, within GFF1737 at 396.847 kb on + strand, within GFF1737 at 396.847 kb on + strand, within GFF1737 at 396.847 kb on + strand, within GFF1737 at 396.848 kb on - strand, within GFF1737 at 396.848 kb on - strand, within GFF1737 at 396.848 kb on - strand, within GFF1737 at 396.856 kb on + strand, within GFF1737 at 396.857 kb on - strand, within GFF1737 at 396.857 kb on - strand, within GFF1737 at 396.857 kb on - strand, within GFF1737 at 397.010 kb on - strand, within GFF1737 at 397.063 kb on + strand, within GFF1737 at 397.063 kb on + strand, within GFF1737 at 397.390 kb on + strand, within GFF1737 at 397.391 kb on - strand, within GFF1737 at 397.391 kb on - strand, within GFF1737 at 397.391 kb on - strand, within GFF1737 at 397.621 kb on + strand, within GFF1737 at 397.621 kb on + strand, within GFF1737 at 397.668 kb on - strand, within GFF1737 at 397.807 kb on + strand, within GFF1737 at 397.808 kb on - strand, within GFF1737 at 397.940 kb on - strand, within GFF1737 at 398.057 kb on - strand, within GFF1737 at 398.194 kb on + strand at 398.194 kb on + strand at 398.195 kb on - strand at 398.324 kb on + strand, within GFF1738 at 398.379 kb on - strand, within GFF1738 at 398.388 kb on - strand, within GFF1738 at 398.435 kb on + strand, within GFF1738 at 398.498 kb on + strand at 398.499 kb on - strand at 398.499 kb on - strand at 398.559 kb on + strand at 398.559 kb on + strand at 398.559 kb on + strand at 398.560 kb on - strand at 398.560 kb on - strand at 398.560 kb on - strand at 398.785 kb on + strand, within GFF1739 at 398.786 kb on - strand, within GFF1739 at 398.786 kb on - strand, within GFF1739 at 398.786 kb on - strand, within GFF1739 at 399.049 kb on + strand, within GFF1739 at 399.049 kb on + strand, within GFF1739 at 399.050 kb on - strand, within GFF1739 at 399.050 kb on - strand, within GFF1739 at 399.050 kb on - strand, within GFF1739 at 399.050 kb on - strand, within GFF1739 at 399.119 kb on - strand, within GFF1739 at 399.119 kb on - strand, within GFF1739 at 399.157 kb on + strand, within GFF1739
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 395,304 + GFF1736 0.45 -0.2 395,305 - GFF1736 0.45 -0.1 395,305 - GFF1736 0.45 -1.6 395,421 + GFF1736 0.54 +0.1 395,422 - GFF1736 0.54 -0.5 395,810 + GFF1736 0.84 -0.7 396,042 + +0.3 396,042 + -0.1 396,043 - -0.5 396,043 - +0.2 396,043 - -1.1 396,043 - +1.7 396,073 + -0.6 396,073 + -0.7 396,355 + -0.1 396,355 + -0.4 396,355 + -0.4 396,355 + -0.5 396,355 + -0.4 396,356 - +0.7 396,356 - -0.4 396,368 - +0.4 396,373 + -0.4 396,373 + +1.4 396,376 + -0.3 396,376 + -0.3 396,376 + -0.9 396,376 + +0.1 396,376 + -0.1 396,377 - -0.1 396,461 - GFF1737 0.12 +0.2 396,559 + GFF1737 0.16 -1.0 396,560 - GFF1737 0.16 +0.8 396,847 + GFF1737 0.30 +0.7 396,847 + GFF1737 0.30 +0.7 396,847 + GFF1737 0.30 -0.7 396,848 - GFF1737 0.30 -0.3 396,848 - GFF1737 0.30 +0.6 396,848 - GFF1737 0.30 +0.2 396,856 + GFF1737 0.31 +0.1 396,857 - GFF1737 0.31 +0.8 396,857 - GFF1737 0.31 +0.1 396,857 - GFF1737 0.31 -0.0 397,010 - GFF1737 0.38 +1.1 397,063 + GFF1737 0.41 +0.5 397,063 + GFF1737 0.41 +0.4 397,390 + GFF1737 0.56 -0.1 397,391 - GFF1737 0.57 +0.1 397,391 - GFF1737 0.57 -0.4 397,391 - GFF1737 0.57 -0.6 397,621 + GFF1737 0.68 -0.2 397,621 + GFF1737 0.68 +0.9 397,668 - GFF1737 0.70 -0.9 397,807 + GFF1737 0.77 +0.4 397,808 - GFF1737 0.77 -0.3 397,940 - GFF1737 0.83 +0.1 398,057 - GFF1737 0.89 -0.1 398,194 + -0.1 398,194 + +0.5 398,195 - -0.5 398,324 + GFF1738 0.14 -0.3 398,379 - GFF1738 0.41 +0.1 398,388 - GFF1738 0.45 +0.0 398,435 + GFF1738 0.68 -0.7 398,498 + -0.0 398,499 - -0.4 398,499 - -0.4 398,559 + +0.1 398,559 + -0.5 398,559 + +0.3 398,560 - +0.9 398,560 - -0.9 398,560 - +0.3 398,785 + GFF1739 0.12 -0.5 398,786 - GFF1739 0.12 -0.4 398,786 - GFF1739 0.12 -0.4 398,786 - GFF1739 0.12 -1.1 399,049 + GFF1739 0.33 -1.3 399,049 + GFF1739 0.33 -0.2 399,050 - GFF1739 0.33 +1.7 399,050 - GFF1739 0.33 +0.0 399,050 - GFF1739 0.33 -0.1 399,050 - GFF1739 0.33 -2.1 399,119 - GFF1739 0.38 -0.8 399,119 - GFF1739 0.38 -0.3 399,157 + GFF1739 0.41 -0.0
Or see this region's nucleotide sequence