Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF159 and GFF160 are separated by 103 nucleotides GFF160 and GFF161 are separated by 2 nucleotides GFF161 and GFF162 are separated by 10 nucleotides
GFF159 - Acyl-CoA dehydrogenase/oxidase domain protein, at 186,846 to 187,865
GFF159
GFF160 - hypothetical protein, at 187,969 to 188,559
GFF160
GFF161 - hypothetical protein, at 188,562 to 189,185
GFF161
GFF162 - hypothetical protein, at 189,196 to 190,413
GFF162
Position (kb) 187
188
189 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 187.163 kb on + strand, within GFF159 at 187.163 kb on + strand, within GFF159 at 187.163 kb on + strand, within GFF159 at 187.163 kb on + strand, within GFF159 at 187.163 kb on + strand, within GFF159 at 187.163 kb on + strand, within GFF159 at 187.164 kb on - strand, within GFF159 at 187.303 kb on + strand, within GFF159 at 187.303 kb on + strand, within GFF159 at 187.303 kb on + strand, within GFF159 at 187.303 kb on + strand, within GFF159 at 187.304 kb on - strand, within GFF159 at 187.304 kb on - strand, within GFF159 at 187.304 kb on - strand, within GFF159 at 187.304 kb on - strand, within GFF159 at 187.839 kb on + strand at 187.839 kb on + strand at 187.839 kb on + strand at 187.839 kb on + strand at 187.839 kb on + strand at 187.839 kb on + strand at 187.839 kb on + strand at 187.839 kb on + strand at 187.839 kb on + strand at 187.839 kb on + strand at 187.839 kb on + strand at 187.839 kb on + strand at 187.839 kb on + strand at 187.839 kb on + strand at 187.839 kb on + strand at 187.840 kb on - strand at 187.840 kb on - strand at 187.840 kb on - strand at 187.840 kb on - strand at 187.840 kb on - strand at 187.840 kb on - strand at 187.840 kb on - strand at 187.840 kb on - strand at 187.840 kb on - strand at 187.840 kb on - strand at 187.840 kb on - strand at 187.840 kb on - strand at 187.840 kb on - strand at 187.840 kb on - strand at 187.840 kb on - strand at 187.840 kb on - strand at 187.840 kb on - strand at 187.840 kb on - strand at 187.840 kb on - strand at 187.840 kb on - strand at 187.877 kb on - strand at 187.924 kb on + strand at 187.924 kb on + strand at 187.925 kb on - strand at 187.925 kb on - strand at 187.925 kb on - strand at 187.925 kb on - strand at 187.925 kb on - strand at 187.925 kb on - strand at 187.925 kb on - strand at 188.168 kb on + strand, within GFF160 at 188.169 kb on - strand, within GFF160 at 188.177 kb on + strand, within GFF160 at 188.178 kb on - strand, within GFF160 at 188.264 kb on + strand, within GFF160 at 188.265 kb on - strand, within GFF160 at 188.265 kb on - strand, within GFF160 at 188.402 kb on + strand, within GFF160 at 188.402 kb on + strand, within GFF160 at 188.403 kb on - strand, within GFF160 at 188.403 kb on - strand, within GFF160 at 188.403 kb on - strand, within GFF160 at 188.403 kb on - strand, within GFF160 at 188.574 kb on - strand at 188.596 kb on + strand at 188.596 kb on + strand at 188.596 kb on + strand at 188.596 kb on + strand at 188.597 kb on - strand at 188.597 kb on - strand at 188.662 kb on + strand, within GFF161 at 188.662 kb on + strand, within GFF161 at 188.662 kb on + strand, within GFF161 at 188.662 kb on + strand, within GFF161 at 188.663 kb on - strand, within GFF161 at 188.663 kb on - strand, within GFF161 at 188.663 kb on - strand, within GFF161 at 188.663 kb on - strand, within GFF161 at 188.728 kb on + strand, within GFF161 at 188.728 kb on + strand, within GFF161 at 188.728 kb on + strand, within GFF161 at 188.728 kb on + strand, within GFF161 at 188.728 kb on + strand, within GFF161 at 188.728 kb on + strand, within GFF161 at 188.728 kb on + strand, within GFF161 at 188.728 kb on + strand, within GFF161 at 188.728 kb on + strand, within GFF161 at 188.729 kb on - strand, within GFF161 at 188.729 kb on - strand, within GFF161 at 188.729 kb on - strand, within GFF161 at 188.729 kb on - strand, within GFF161 at 188.729 kb on - strand, within GFF161 at 188.729 kb on - strand, within GFF161 at 188.729 kb on - strand, within GFF161 at 188.735 kb on + strand, within GFF161 at 188.736 kb on - strand, within GFF161 at 188.819 kb on + strand, within GFF161 at 188.819 kb on + strand, within GFF161 at 188.820 kb on - strand, within GFF161 at 188.820 kb on - strand, within GFF161 at 188.820 kb on - strand, within GFF161 at 188.959 kb on + strand, within GFF161 at 188.959 kb on + strand, within GFF161 at 188.959 kb on + strand, within GFF161 at 188.959 kb on + strand, within GFF161 at 188.959 kb on + strand, within GFF161 at 188.959 kb on + strand, within GFF161 at 188.959 kb on + strand, within GFF161 at 188.959 kb on + strand, within GFF161 at 188.959 kb on + strand, within GFF161 at 188.960 kb on - strand, within GFF161 at 188.960 kb on - strand, within GFF161 at 188.960 kb on - strand, within GFF161 at 188.960 kb on - strand, within GFF161 at 188.998 kb on + strand, within GFF161 at 188.998 kb on + strand, within GFF161 at 188.998 kb on + strand, within GFF161 at 188.998 kb on + strand, within GFF161 at 188.998 kb on + strand, within GFF161 at 188.998 kb on + strand, within GFF161 at 188.998 kb on + strand, within GFF161 at 188.998 kb on + strand, within GFF161 at 188.998 kb on + strand, within GFF161 at 188.998 kb on + strand, within GFF161 at 188.998 kb on + strand, within GFF161 at 188.999 kb on - strand, within GFF161 at 188.999 kb on - strand, within GFF161 at 188.999 kb on - strand, within GFF161 at 188.999 kb on - strand, within GFF161 at 188.999 kb on - strand, within GFF161 at 188.999 kb on - strand, within GFF161 at 188.999 kb on - strand, within GFF161 at 188.999 kb on - strand, within GFF161 at 188.999 kb on - strand, within GFF161 at 188.999 kb on - strand, within GFF161 at 188.999 kb on - strand, within GFF161 at 189.007 kb on + strand, within GFF161 at 189.007 kb on + strand, within GFF161 at 189.007 kb on + strand, within GFF161 at 189.007 kb on + strand, within GFF161 at 189.007 kb on + strand, within GFF161 at 189.007 kb on + strand, within GFF161 at 189.007 kb on + strand, within GFF161 at 189.007 kb on + strand, within GFF161 at 189.008 kb on - strand, within GFF161 at 189.008 kb on - strand, within GFF161 at 189.008 kb on - strand, within GFF161 at 189.008 kb on - strand, within GFF161 at 189.008 kb on - strand, within GFF161 at 189.008 kb on - strand, within GFF161 at 189.088 kb on + strand, within GFF161 at 189.088 kb on + strand, within GFF161 at 189.243 kb on + strand at 189.243 kb on + strand at 189.243 kb on + strand at 189.244 kb on - strand at 189.244 kb on - strand at 189.263 kb on + strand at 189.263 kb on + strand at 189.264 kb on - strand at 189.264 kb on - strand at 189.264 kb on - strand at 189.264 kb on - strand at 189.326 kb on + strand, within GFF162 at 189.326 kb on + strand, within GFF162 at 189.326 kb on + strand, within GFF162 at 189.326 kb on + strand, within GFF162 at 189.327 kb on - strand, within GFF162 at 189.327 kb on - strand, within GFF162 at 189.327 kb on - strand, within GFF162 at 189.327 kb on - strand, within GFF162 at 189.327 kb on - strand, within GFF162 at 189.327 kb on - strand, within GFF162 at 189.327 kb on - strand, within GFF162 at 189.327 kb on - strand, within GFF162 at 189.327 kb on - strand, within GFF162 at 189.327 kb on - strand, within GFF162 at 189.327 kb on - strand, within GFF162 at 189.327 kb on - strand, within GFF162 at 189.327 kb on - strand, within GFF162 at 189.327 kb on - strand, within GFF162 at 189.383 kb on + strand, within GFF162 at 189.383 kb on + strand, within GFF162 at 189.383 kb on + strand, within GFF162 at 189.383 kb on + strand, within GFF162 at 189.383 kb on + strand, within GFF162 at 189.383 kb on + strand, within GFF162 at 189.384 kb on - strand, within GFF162 at 189.384 kb on - strand, within GFF162 at 189.384 kb on - strand, within GFF162 at 189.384 kb on - strand, within GFF162 at 189.384 kb on - strand, within GFF162 at 189.392 kb on + strand, within GFF162 at 189.393 kb on - strand, within GFF162 at 189.423 kb on + strand, within GFF162 at 189.423 kb on + strand, within GFF162 at 189.423 kb on + strand, within GFF162 at 189.423 kb on + strand, within GFF162 at 189.423 kb on + strand, within GFF162 at 189.423 kb on + strand, within GFF162 at 189.423 kb on + strand, within GFF162 at 189.423 kb on + strand, within GFF162 at 189.424 kb on - strand, within GFF162 at 189.424 kb on - strand, within GFF162 at 189.424 kb on - strand, within GFF162 at 189.424 kb on - strand, within GFF162 at 189.424 kb on - strand, within GFF162 at 189.424 kb on - strand, within GFF162 at 189.424 kb on - strand, within GFF162 at 189.424 kb on - strand, within GFF162 at 189.424 kb on - strand, within GFF162 at 189.424 kb on - strand, within GFF162 at 189.424 kb on - strand, within GFF162 at 189.498 kb on + strand, within GFF162 at 189.498 kb on + strand, within GFF162 at 189.498 kb on + strand, within GFF162 at 189.498 kb on + strand, within GFF162 at 189.498 kb on + strand, within GFF162 at 189.498 kb on + strand, within GFF162 at 189.498 kb on + strand, within GFF162 at 189.498 kb on + strand, within GFF162 at 189.498 kb on + strand, within GFF162 at 189.498 kb on + strand, within GFF162 at 189.498 kb on + strand, within GFF162 at 189.498 kb on + strand, within GFF162 at 189.498 kb on + strand, within GFF162 at 189.498 kb on + strand, within GFF162 at 189.498 kb on + strand, within GFF162 at 189.499 kb on - strand, within GFF162 at 189.499 kb on - strand, within GFF162 at 189.499 kb on - strand, within GFF162 at 189.499 kb on - strand, within GFF162 at 189.499 kb on - strand, within GFF162 at 189.499 kb on - strand, within GFF162 at 189.499 kb on - strand, within GFF162 at 189.499 kb on - strand, within GFF162 at 189.499 kb on - strand, within GFF162 at 189.499 kb on - strand, within GFF162 at 189.499 kb on - strand, within GFF162 at 189.500 kb on + strand, within GFF162 at 189.500 kb on + strand, within GFF162 at 189.500 kb on + strand, within GFF162 at 189.501 kb on - strand, within GFF162 at 189.501 kb on - strand, within GFF162 at 189.501 kb on - strand, within GFF162 at 189.501 kb on - strand, within GFF162 at 189.501 kb on - strand, within GFF162
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 187,163 + GFF159 0.31 +0.3 187,163 + GFF159 0.31 -0.2 187,163 + GFF159 0.31 -0.5 187,163 + GFF159 0.31 +0.1 187,163 + GFF159 0.31 -0.3 187,163 + GFF159 0.31 +0.5 187,164 - GFF159 0.31 +0.7 187,303 + GFF159 0.45 -0.3 187,303 + GFF159 0.45 +0.3 187,303 + GFF159 0.45 +0.1 187,303 + GFF159 0.45 +0.3 187,304 - GFF159 0.45 -0.5 187,304 - GFF159 0.45 +0.3 187,304 - GFF159 0.45 +0.1 187,304 - GFF159 0.45 -0.2 187,839 + +1.4 187,839 + -0.2 187,839 + -0.9 187,839 + -1.0 187,839 + -0.4 187,839 + -1.1 187,839 + -0.2 187,839 + +0.5 187,839 + +1.7 187,839 + +1.8 187,839 + -0.7 187,839 + -0.7 187,839 + -0.4 187,839 + -0.4 187,839 + -0.1 187,840 - -0.0 187,840 - +0.7 187,840 - +1.3 187,840 - -0.2 187,840 - -2.4 187,840 - -0.3 187,840 - +0.7 187,840 - -0.5 187,840 - -0.6 187,840 - +0.7 187,840 - -0.5 187,840 - +0.4 187,840 - +0.1 187,840 - -2.8 187,840 - -0.7 187,840 - -1.3 187,840 - -0.5 187,840 - -0.2 187,840 - +0.4 187,840 - -1.5 187,877 - -0.3 187,924 + -0.3 187,924 + -0.0 187,925 - -0.5 187,925 - +0.3 187,925 - -0.1 187,925 - -0.2 187,925 - +0.5 187,925 - -0.6 187,925 - -0.1 188,168 + GFF160 0.34 +0.8 188,169 - GFF160 0.34 -2.7 188,177 + GFF160 0.35 +0.8 188,178 - GFF160 0.35 -1.4 188,264 + GFF160 0.50 -1.0 188,265 - GFF160 0.50 -0.5 188,265 - GFF160 0.50 -0.5 188,402 + GFF160 0.73 +2.3 188,402 + GFF160 0.73 -0.3 188,403 - GFF160 0.73 -0.6 188,403 - GFF160 0.73 +0.5 188,403 - GFF160 0.73 +0.5 188,403 - GFF160 0.73 +0.7 188,574 - -0.5 188,596 + -0.3 188,596 + -1.3 188,596 + -0.7 188,596 + -0.4 188,597 - -0.1 188,597 - +0.8 188,662 + GFF161 0.16 -0.7 188,662 + GFF161 0.16 +0.1 188,662 + GFF161 0.16 -0.3 188,662 + GFF161 0.16 -0.4 188,663 - GFF161 0.16 +1.8 188,663 - GFF161 0.16 -0.6 188,663 - GFF161 0.16 -1.2 188,663 - GFF161 0.16 +0.4 188,728 + GFF161 0.27 +0.2 188,728 + GFF161 0.27 +0.9 188,728 + GFF161 0.27 -0.2 188,728 + GFF161 0.27 -0.1 188,728 + GFF161 0.27 -1.7 188,728 + GFF161 0.27 +0.7 188,728 + GFF161 0.27 -1.0 188,728 + GFF161 0.27 -0.6 188,728 + GFF161 0.27 +1.0 188,729 - GFF161 0.27 -0.2 188,729 - GFF161 0.27 +0.1 188,729 - GFF161 0.27 -1.1 188,729 - GFF161 0.27 -0.9 188,729 - GFF161 0.27 -1.0 188,729 - GFF161 0.27 -0.7 188,729 - GFF161 0.27 -0.5 188,735 + GFF161 0.28 -1.4 188,736 - GFF161 0.28 -1.1 188,819 + GFF161 0.41 +0.2 188,819 + GFF161 0.41 -0.1 188,820 - GFF161 0.41 +0.6 188,820 - GFF161 0.41 -0.6 188,820 - GFF161 0.41 -2.5 188,959 + GFF161 0.64 -0.6 188,959 + GFF161 0.64 +0.4 188,959 + GFF161 0.64 -0.6 188,959 + GFF161 0.64 -0.6 188,959 + GFF161 0.64 -0.5 188,959 + GFF161 0.64 +1.3 188,959 + GFF161 0.64 -1.0 188,959 + GFF161 0.64 -0.1 188,959 + GFF161 0.64 -1.0 188,960 - GFF161 0.64 -0.6 188,960 - GFF161 0.64 +0.2 188,960 - GFF161 0.64 +1.1 188,960 - GFF161 0.64 -0.5 188,998 + GFF161 0.70 +0.4 188,998 + GFF161 0.70 -0.5 188,998 + GFF161 0.70 -0.6 188,998 + GFF161 0.70 +0.2 188,998 + GFF161 0.70 +1.0 188,998 + GFF161 0.70 +0.5 188,998 + GFF161 0.70 -0.2 188,998 + GFF161 0.70 -0.1 188,998 + GFF161 0.70 -1.1 188,998 + GFF161 0.70 +0.2 188,998 + GFF161 0.70 -0.9 188,999 - GFF161 0.70 -2.3 188,999 - GFF161 0.70 +0.3 188,999 - GFF161 0.70 -0.5 188,999 - GFF161 0.70 +0.9 188,999 - GFF161 0.70 -1.1 188,999 - GFF161 0.70 +0.0 188,999 - GFF161 0.70 -0.6 188,999 - GFF161 0.70 +0.2 188,999 - GFF161 0.70 -0.9 188,999 - GFF161 0.70 +0.3 188,999 - GFF161 0.70 +0.5 189,007 + GFF161 0.71 -2.1 189,007 + GFF161 0.71 -0.3 189,007 + GFF161 0.71 -2.4 189,007 + GFF161 0.71 +0.4 189,007 + GFF161 0.71 -0.8 189,007 + GFF161 0.71 -0.9 189,007 + GFF161 0.71 -1.2 189,007 + GFF161 0.71 -1.3 189,008 - GFF161 0.71 -0.2 189,008 - GFF161 0.71 -0.3 189,008 - GFF161 0.71 +1.3 189,008 - GFF161 0.71 -0.4 189,008 - GFF161 0.71 -0.2 189,008 - GFF161 0.71 -1.1 189,088 + GFF161 0.84 -1.6 189,088 + GFF161 0.84 +0.4 189,243 + +0.4 189,243 + -0.6 189,243 + +0.6 189,244 - -1.2 189,244 - -0.1 189,263 + -1.0 189,263 + +0.8 189,264 - +0.9 189,264 - +0.3 189,264 - -0.5 189,264 - -0.0 189,326 + GFF162 0.11 -0.1 189,326 + GFF162 0.11 -1.2 189,326 + GFF162 0.11 +0.3 189,326 + GFF162 0.11 -0.7 189,327 - GFF162 0.11 +1.3 189,327 - GFF162 0.11 +0.4 189,327 - GFF162 0.11 -0.5 189,327 - GFF162 0.11 +0.4 189,327 - GFF162 0.11 +0.2 189,327 - GFF162 0.11 -0.2 189,327 - GFF162 0.11 -0.5 189,327 - GFF162 0.11 +2.3 189,327 - GFF162 0.11 -0.3 189,327 - GFF162 0.11 -1.8 189,327 - GFF162 0.11 +1.3 189,327 - GFF162 0.11 +1.1 189,327 - GFF162 0.11 +0.3 189,327 - GFF162 0.11 +0.1 189,383 + GFF162 0.15 +0.1 189,383 + GFF162 0.15 -0.2 189,383 + GFF162 0.15 -0.2 189,383 + GFF162 0.15 -0.4 189,383 + GFF162 0.15 +0.1 189,383 + GFF162 0.15 +0.3 189,384 - GFF162 0.15 -0.3 189,384 - GFF162 0.15 +0.6 189,384 - GFF162 0.15 +0.6 189,384 - GFF162 0.15 -0.5 189,384 - GFF162 0.15 -0.4 189,392 + GFF162 0.16 -0.6 189,393 - GFF162 0.16 +0.9 189,423 + GFF162 0.19 -1.3 189,423 + GFF162 0.19 -0.6 189,423 + GFF162 0.19 +0.2 189,423 + GFF162 0.19 +0.5 189,423 + GFF162 0.19 -0.9 189,423 + GFF162 0.19 -1.3 189,423 + GFF162 0.19 +1.3 189,423 + GFF162 0.19 -1.4 189,424 - GFF162 0.19 +0.4 189,424 - GFF162 0.19 -1.6 189,424 - GFF162 0.19 -0.5 189,424 - GFF162 0.19 -1.3 189,424 - GFF162 0.19 +0.9 189,424 - GFF162 0.19 +0.2 189,424 - GFF162 0.19 -1.1 189,424 - GFF162 0.19 +0.0 189,424 - GFF162 0.19 +0.4 189,424 - GFF162 0.19 +0.0 189,424 - GFF162 0.19 -1.0 189,498 + GFF162 0.25 -1.6 189,498 + GFF162 0.25 +1.2 189,498 + GFF162 0.25 +0.1 189,498 + GFF162 0.25 +1.6 189,498 + GFF162 0.25 -0.1 189,498 + GFF162 0.25 +0.0 189,498 + GFF162 0.25 -0.1 189,498 + GFF162 0.25 +0.0 189,498 + GFF162 0.25 +0.1 189,498 + GFF162 0.25 -1.2 189,498 + GFF162 0.25 +0.1 189,498 + GFF162 0.25 +0.3 189,498 + GFF162 0.25 -0.2 189,498 + GFF162 0.25 +1.3 189,498 + GFF162 0.25 -1.1 189,499 - GFF162 0.25 +0.6 189,499 - GFF162 0.25 +0.2 189,499 - GFF162 0.25 -0.1 189,499 - GFF162 0.25 -0.2 189,499 - GFF162 0.25 +1.7 189,499 - GFF162 0.25 +0.8 189,499 - GFF162 0.25 +0.7 189,499 - GFF162 0.25 -0.6 189,499 - GFF162 0.25 -0.3 189,499 - GFF162 0.25 -0.4 189,499 - GFF162 0.25 +0.5 189,500 + GFF162 0.25 -0.8 189,500 + GFF162 0.25 -0.1 189,500 + GFF162 0.25 -0.7 189,501 - GFF162 0.25 -0.2 189,501 - GFF162 0.25 +0.3 189,501 - GFF162 0.25 -0.1 189,501 - GFF162 0.25 +1.0 189,501 - GFF162 0.25 -0.4
Or see this region's nucleotide sequence