Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1580 and GFF1581 overlap by 14 nucleotides GFF1581 and GFF1582 are separated by 20 nucleotides GFF1582 and GFF1583 are separated by 22 nucleotides GFF1583 and GFF1584 are separated by 6 nucleotides
GFF1580 - Mhp operon transcriptional activator, at 179,611 to 180,378
GFF1580
GFF1581 - 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase (EC 4.1.2.52), at 180,365 to 181,114
GFF1581
GFF1582 - BUG/TctC family periplasmic protein, at 181,135 to 182,139
GFF1582
GFF1583 - Fumarate hydratase class I, beta region (EC 4.2.1.2); L(+)-tartrate dehydratase beta subunit (EC 4.2.1.32), at 182,162 to 182,779
GFF1583
GFF1584 - Fumarate hydratase class I, alpha region (EC 4.2.1.2); L(+)-tartrate dehydratase alpha subunit (EC 4.2.1.32), at 182,786 to 183,658
GFF1584
Position (kb) 181
182
183 Strain fitness (log2 ratio) -1
0
1
2 at 180.181 kb on + strand, within GFF1580 at 180.182 kb on - strand, within GFF1580 at 180.376 kb on + strand at 180.377 kb on - strand at 180.377 kb on - strand at 181.120 kb on + strand at 181.121 kb on - strand at 181.121 kb on - strand at 181.121 kb on - strand at 181.121 kb on - strand at 181.553 kb on + strand, within GFF1582 at 181.554 kb on - strand, within GFF1582 at 181.796 kb on + strand, within GFF1582 at 181.797 kb on - strand, within GFF1582 at 181.847 kb on + strand, within GFF1582 at 182.331 kb on + strand, within GFF1583 at 182.409 kb on + strand, within GFF1583 at 182.409 kb on + strand, within GFF1583 at 182.409 kb on + strand, within GFF1583 at 182.409 kb on + strand, within GFF1583 at 182.410 kb on - strand, within GFF1583 at 182.548 kb on - strand, within GFF1583 at 182.787 kb on + strand at 182.788 kb on - strand at 182.820 kb on + strand at 182.820 kb on + strand at 182.821 kb on - strand at 182.876 kb on + strand, within GFF1584
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 180,181 + GFF1580 0.74 +1.4 180,182 - GFF1580 0.74 -0.9 180,376 + -0.0 180,377 - +0.5 180,377 - -0.9 181,120 + -0.1 181,121 - -1.2 181,121 - -1.3 181,121 - +2.0 181,121 - -1.1 181,553 + GFF1582 0.42 -0.6 181,554 - GFF1582 0.42 -1.5 181,796 + GFF1582 0.66 -0.1 181,797 - GFF1582 0.66 -0.3 181,847 + GFF1582 0.71 +1.2 182,331 + GFF1583 0.27 -0.4 182,409 + GFF1583 0.40 +1.1 182,409 + GFF1583 0.40 +0.9 182,409 + GFF1583 0.40 +0.1 182,409 + GFF1583 0.40 -0.7 182,410 - GFF1583 0.40 -0.1 182,548 - GFF1583 0.62 +1.7 182,787 + -0.3 182,788 - +0.6 182,820 + -0.1 182,820 + +0.3 182,821 - +1.3 182,876 + GFF1584 0.10 +0.1
Or see this region's nucleotide sequence