Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1480 and GFF1481 are separated by 66 nucleotides GFF1481 and GFF1482 overlap by 4 nucleotides GFF1482 and GFF1483 overlap by 13 nucleotides
GFF1480 - Choline-sulfatase (EC 3.1.6.6), at 72,040 to 73,563
GFF1480
GFF1481 - L-carnitine dehydratase/bile acid-inducible protein F, at 73,630 to 74,874
GFF1481
GFF1482 - Enoyl-CoA hydratase (EC 4.2.1.17), at 74,871 to 75,632
GFF1482
GFF1483 - Ribulose-5-phosphate 4-epimerase and related epimerases and aldolases, at 75,620 to 76,423
GFF1483
Position (kb) 73
74
75 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 72.780 kb on - strand, within GFF1480 at 72.795 kb on - strand, within GFF1480 at 72.795 kb on - strand, within GFF1480 at 72.797 kb on + strand, within GFF1480 at 72.798 kb on - strand, within GFF1480 at 72.992 kb on + strand, within GFF1480 at 73.080 kb on - strand, within GFF1480 at 73.080 kb on - strand, within GFF1480 at 73.148 kb on + strand, within GFF1480 at 73.148 kb on + strand, within GFF1480 at 73.149 kb on - strand, within GFF1480 at 73.202 kb on + strand, within GFF1480 at 73.202 kb on + strand, within GFF1480 at 73.203 kb on - strand, within GFF1480 at 73.287 kb on - strand, within GFF1480 at 73.287 kb on - strand, within GFF1480 at 73.304 kb on + strand, within GFF1480 at 73.304 kb on + strand, within GFF1480 at 73.305 kb on - strand, within GFF1480 at 73.307 kb on + strand, within GFF1480 at 73.308 kb on - strand, within GFF1480 at 73.308 kb on - strand, within GFF1480 at 73.427 kb on + strand at 73.428 kb on - strand at 73.441 kb on + strand at 73.441 kb on + strand at 73.441 kb on + strand at 73.442 kb on - strand at 73.605 kb on - strand at 73.612 kb on - strand at 73.612 kb on - strand at 73.613 kb on + strand at 73.834 kb on + strand, within GFF1481 at 73.834 kb on + strand, within GFF1481 at 73.834 kb on + strand, within GFF1481 at 73.834 kb on + strand, within GFF1481 at 73.834 kb on + strand, within GFF1481 at 73.837 kb on + strand, within GFF1481 at 73.837 kb on + strand, within GFF1481 at 73.837 kb on + strand, within GFF1481 at 73.837 kb on + strand, within GFF1481 at 73.837 kb on + strand, within GFF1481 at 73.984 kb on + strand, within GFF1481 at 73.984 kb on + strand, within GFF1481 at 73.984 kb on + strand, within GFF1481 at 73.985 kb on - strand, within GFF1481 at 73.985 kb on - strand, within GFF1481 at 73.985 kb on - strand, within GFF1481 at 74.020 kb on + strand, within GFF1481 at 75.273 kb on + strand, within GFF1482 at 75.273 kb on + strand, within GFF1482 at 75.273 kb on + strand, within GFF1482 at 75.276 kb on + strand, within GFF1482 at 75.436 kb on - strand, within GFF1482 at 75.472 kb on + strand, within GFF1482 at 75.472 kb on + strand, within GFF1482 at 75.472 kb on + strand, within GFF1482 at 75.512 kb on + strand, within GFF1482 at 75.543 kb on + strand, within GFF1482 at 75.740 kb on - strand, within GFF1483
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 72,780 - GFF1480 0.49 +0.1 72,795 - GFF1480 0.50 -0.3 72,795 - GFF1480 0.50 +0.0 72,797 + GFF1480 0.50 -0.3 72,798 - GFF1480 0.50 -0.4 72,992 + GFF1480 0.62 -2.3 73,080 - GFF1480 0.68 -0.4 73,080 - GFF1480 0.68 +0.0 73,148 + GFF1480 0.73 +0.1 73,148 + GFF1480 0.73 +0.8 73,149 - GFF1480 0.73 -0.3 73,202 + GFF1480 0.76 -0.1 73,202 + GFF1480 0.76 -0.6 73,203 - GFF1480 0.76 +0.2 73,287 - GFF1480 0.82 +1.0 73,287 - GFF1480 0.82 -1.1 73,304 + GFF1480 0.83 -0.2 73,304 + GFF1480 0.83 -0.6 73,305 - GFF1480 0.83 -0.4 73,307 + GFF1480 0.83 +0.4 73,308 - GFF1480 0.83 +0.1 73,308 - GFF1480 0.83 +1.8 73,427 + +0.2 73,428 - -0.6 73,441 + -0.2 73,441 + -1.2 73,441 + +0.1 73,442 - -0.7 73,605 - +0.7 73,612 - -0.3 73,612 - +0.5 73,613 + -1.3 73,834 + GFF1481 0.16 -0.1 73,834 + GFF1481 0.16 -0.7 73,834 + GFF1481 0.16 +1.7 73,834 + GFF1481 0.16 +1.7 73,834 + GFF1481 0.16 -0.1 73,837 + GFF1481 0.17 -0.5 73,837 + GFF1481 0.17 -0.4 73,837 + GFF1481 0.17 -0.5 73,837 + GFF1481 0.17 -0.4 73,837 + GFF1481 0.17 -1.1 73,984 + GFF1481 0.28 +3.1 73,984 + GFF1481 0.28 -0.0 73,984 + GFF1481 0.28 -0.6 73,985 - GFF1481 0.29 -0.2 73,985 - GFF1481 0.29 -1.4 73,985 - GFF1481 0.29 -0.9 74,020 + GFF1481 0.31 +0.6 75,273 + GFF1482 0.53 +0.7 75,273 + GFF1482 0.53 -2.3 75,273 + GFF1482 0.53 +0.2 75,276 + GFF1482 0.53 +0.8 75,436 - GFF1482 0.74 -0.3 75,472 + GFF1482 0.79 -0.5 75,472 + GFF1482 0.79 +0.1 75,472 + GFF1482 0.79 +0.1 75,512 + GFF1482 0.84 +0.7 75,543 + GFF1482 0.88 +0.3 75,740 - GFF1483 0.15 +2.5
Or see this region's nucleotide sequence