Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1433 and GFF1434 are separated by 21 nucleotides GFF1434 and GFF1435 are separated by 109 nucleotides
GFF1433 - hypothetical protein, at 23,364 to 24,764
GFF1433
GFF1434 - hypothetical protein, at 24,786 to 25,469
GFF1434
GFF1435 - BUG/TctC family periplasmic protein, at 25,579 to 26,580
GFF1435
Position (kb)
24
25
26 Strain fitness (log2 ratio)
-2
-1
0
1 at 23.796 kb on + strand, within GFF1433 at 23.797 kb on - strand, within GFF1433 at 23.797 kb on - strand, within GFF1433 at 24.160 kb on - strand, within GFF1433 at 24.213 kb on + strand, within GFF1433 at 24.214 kb on - strand, within GFF1433 at 24.214 kb on - strand, within GFF1433 at 24.216 kb on + strand, within GFF1433 at 24.340 kb on - strand, within GFF1433 at 24.480 kb on + strand, within GFF1433 at 24.480 kb on + strand, within GFF1433 at 24.481 kb on - strand, within GFF1433 at 24.481 kb on - strand, within GFF1433 at 24.519 kb on + strand, within GFF1433 at 24.519 kb on + strand, within GFF1433 at 24.520 kb on - strand, within GFF1433 at 24.520 kb on - strand, within GFF1433 at 24.520 kb on - strand, within GFF1433 at 24.675 kb on + strand at 25.309 kb on + strand, within GFF1434 at 25.321 kb on + strand, within GFF1434 at 25.321 kb on + strand, within GFF1434 at 25.462 kb on + strand at 25.463 kb on - strand at 25.463 kb on - strand at 25.463 kb on - strand at 25.511 kb on + strand at 25.511 kb on + strand at 25.511 kb on + strand at 25.512 kb on - strand at 25.512 kb on - strand at 25.548 kb on + strand at 25.548 kb on + strand at 26.090 kb on + strand, within GFF1435 at 26.091 kb on - strand, within GFF1435 at 26.195 kb on + strand, within GFF1435 at 26.195 kb on + strand, within GFF1435 at 26.195 kb on + strand, within GFF1435 at 26.195 kb on + strand, within GFF1435 at 26.196 kb on - strand, within GFF1435 at 26.196 kb on - strand, within GFF1435 at 26.234 kb on + strand, within GFF1435 at 26.249 kb on + strand, within GFF1435 at 26.250 kb on - strand, within GFF1435
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 23,796 + GFF1433 0.31 +0.9 23,797 - GFF1433 0.31 +0.7 23,797 - GFF1433 0.31 -0.5 24,160 - GFF1433 0.57 -0.8 24,213 + GFF1433 0.61 +1.2 24,214 - GFF1433 0.61 -0.1 24,214 - GFF1433 0.61 -0.4 24,216 + GFF1433 0.61 -0.6 24,340 - GFF1433 0.70 +0.4 24,480 + GFF1433 0.80 -0.4 24,480 + GFF1433 0.80 -0.6 24,481 - GFF1433 0.80 -0.3 24,481 - GFF1433 0.80 +0.4 24,519 + GFF1433 0.82 -0.0 24,519 + GFF1433 0.82 +0.9 24,520 - GFF1433 0.83 -1.7 24,520 - GFF1433 0.83 -0.6 24,520 - GFF1433 0.83 -0.6 24,675 + -0.5 25,309 + GFF1434 0.76 -0.7 25,321 + GFF1434 0.78 -0.9 25,321 + GFF1434 0.78 -0.7 25,462 + +0.1 25,463 - -0.2 25,463 - -0.1 25,463 - +0.6 25,511 + +0.3 25,511 + +0.9 25,511 + +0.3 25,512 - +0.7 25,512 - -0.7 25,548 + -1.2 25,548 + +0.7 26,090 + GFF1435 0.51 +0.8 26,091 - GFF1435 0.51 -0.3 26,195 + GFF1435 0.61 +0.2 26,195 + GFF1435 0.61 +0.5 26,195 + GFF1435 0.61 -0.3 26,195 + GFF1435 0.61 -2.5 26,196 - GFF1435 0.62 -0.9 26,196 - GFF1435 0.62 -0.3 26,234 + GFF1435 0.65 -0.5 26,249 + GFF1435 0.67 +0.8 26,250 - GFF1435 0.67 +0.4
Or see this region's nucleotide sequence