Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1430 and GFF1431 are separated by 97 nucleotides GFF1431 and GFF1432 overlap by 4 nucleotides GFF1432 and GFF1433 are separated by 62 nucleotides
GFF1430 - hypothetical protein, at 19,555 to 21,840
GFF1430
GFF1431 - no description, at 21,938 to 22,504
GFF1431
GFF1432 - no description, at 22,501 to 23,301
GFF1432
GFF1433 - hypothetical protein, at 23,364 to 24,764
GFF1433
Position (kb)
22
23
24 Strain fitness (log2 ratio)
-2
-1
0
1 at 21.772 kb on + strand at 21.860 kb on + strand at 21.861 kb on - strand at 21.861 kb on - strand at 21.861 kb on - strand at 21.867 kb on - strand at 21.867 kb on - strand at 22.062 kb on - strand, within GFF1431 at 22.064 kb on + strand, within GFF1431 at 22.106 kb on + strand, within GFF1431 at 22.106 kb on + strand, within GFF1431 at 22.106 kb on + strand, within GFF1431 at 22.452 kb on + strand at 22.651 kb on + strand, within GFF1432 at 23.215 kb on + strand, within GFF1432 at 23.216 kb on - strand, within GFF1432 at 23.216 kb on - strand, within GFF1432 at 23.440 kb on - strand at 23.547 kb on + strand, within GFF1433 at 23.548 kb on - strand, within GFF1433 at 23.548 kb on - strand, within GFF1433 at 23.548 kb on - strand, within GFF1433 at 23.548 kb on - strand, within GFF1433 at 23.796 kb on + strand, within GFF1433 at 23.797 kb on - strand, within GFF1433 at 23.797 kb on - strand, within GFF1433 at 24.160 kb on - strand, within GFF1433 at 24.213 kb on + strand, within GFF1433 at 24.214 kb on - strand, within GFF1433 at 24.214 kb on - strand, within GFF1433 at 24.216 kb on + strand, within GFF1433
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 21,772 + -2.7 21,860 + -0.8 21,861 - +0.5 21,861 - -0.3 21,861 - +0.3 21,867 - -0.8 21,867 - -0.5 22,062 - GFF1431 0.22 -1.1 22,064 + GFF1431 0.22 -0.0 22,106 + GFF1431 0.30 +0.7 22,106 + GFF1431 0.30 -0.0 22,106 + GFF1431 0.30 +0.5 22,452 + +1.4 22,651 + GFF1432 0.19 -1.2 23,215 + GFF1432 0.89 -0.4 23,216 - GFF1432 0.89 -0.4 23,216 - GFF1432 0.89 +0.2 23,440 - -0.7 23,547 + GFF1433 0.13 -0.6 23,548 - GFF1433 0.13 -0.5 23,548 - GFF1433 0.13 +0.8 23,548 - GFF1433 0.13 -0.5 23,548 - GFF1433 0.13 -0.3 23,796 + GFF1433 0.31 +0.9 23,797 - GFF1433 0.31 +0.7 23,797 - GFF1433 0.31 -0.5 24,160 - GFF1433 0.57 -0.8 24,213 + GFF1433 0.61 +1.2 24,214 - GFF1433 0.61 -0.1 24,214 - GFF1433 0.61 -0.4 24,216 + GFF1433 0.61 -0.6
Or see this region's nucleotide sequence