Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1369 and GFF1370 are separated by 122 nucleotides GFF1370 and GFF1371 are separated by 15 nucleotides
GFF1369 - Serine--glyoxylate aminotransferase (EC 2.6.1.45), at 713,333 to 714,574
GFF1369
GFF1370 - Transcriptional regulator associated with Tricarboxylic transport, at 714,697 to 715,389
GFF1370
GFF1371 - Fe-S protein, homolog of lactate dehydrogenase SO1521, at 715,405 to 718,482
GFF1371
Position (kb) 714
715
716 Strain fitness (log2 ratio) -2
-1
0
1 at 713.773 kb on - strand, within GFF1369 at 713.805 kb on + strand, within GFF1369 at 713.805 kb on + strand, within GFF1369 at 714.303 kb on + strand, within GFF1369 at 714.303 kb on + strand, within GFF1369 at 714.303 kb on + strand, within GFF1369 at 714.303 kb on + strand, within GFF1369 at 714.304 kb on - strand, within GFF1369 at 714.304 kb on - strand, within GFF1369 at 714.304 kb on - strand, within GFF1369 at 714.304 kb on - strand, within GFF1369 at 714.304 kb on - strand, within GFF1369 at 714.379 kb on - strand, within GFF1369 at 714.379 kb on - strand, within GFF1369 at 714.641 kb on + strand at 714.641 kb on + strand at 715.049 kb on - strand, within GFF1370 at 715.090 kb on + strand, within GFF1370 at 715.090 kb on + strand, within GFF1370 at 715.090 kb on + strand, within GFF1370 at 715.090 kb on + strand, within GFF1370 at 715.090 kb on + strand, within GFF1370 at 715.094 kb on - strand, within GFF1370 at 715.162 kb on + strand, within GFF1370 at 715.163 kb on - strand, within GFF1370 at 715.163 kb on - strand, within GFF1370 at 715.367 kb on - strand at 715.543 kb on + strand at 715.544 kb on - strand at 715.564 kb on + strand at 715.565 kb on - strand at 715.837 kb on + strand, within GFF1371 at 715.838 kb on - strand, within GFF1371 at 715.838 kb on - strand, within GFF1371 at 716.152 kb on + strand, within GFF1371 at 716.153 kb on - strand, within GFF1371 at 716.215 kb on + strand, within GFF1371
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 713,773 - GFF1369 0.35 +0.7 713,805 + GFF1369 0.38 -1.5 713,805 + GFF1369 0.38 -0.5 714,303 + GFF1369 0.78 +0.2 714,303 + GFF1369 0.78 -1.2 714,303 + GFF1369 0.78 -0.8 714,303 + GFF1369 0.78 -0.3 714,304 - GFF1369 0.78 -0.3 714,304 - GFF1369 0.78 -2.1 714,304 - GFF1369 0.78 -0.3 714,304 - GFF1369 0.78 +0.1 714,304 - GFF1369 0.78 -0.9 714,379 - GFF1369 0.84 +0.5 714,379 - GFF1369 0.84 +0.3 714,641 + -1.3 714,641 + +0.6 715,049 - GFF1370 0.51 -1.1 715,090 + GFF1370 0.57 -0.3 715,090 + GFF1370 0.57 +0.3 715,090 + GFF1370 0.57 +0.3 715,090 + GFF1370 0.57 -1.2 715,090 + GFF1370 0.57 -0.7 715,094 - GFF1370 0.57 -1.1 715,162 + GFF1370 0.67 -0.5 715,163 - GFF1370 0.67 -0.8 715,163 - GFF1370 0.67 +0.2 715,367 - -1.3 715,543 + -0.8 715,544 - +0.3 715,564 + -0.9 715,565 - -0.5 715,837 + GFF1371 0.14 +1.3 715,838 - GFF1371 0.14 +0.7 715,838 - GFF1371 0.14 -0.4 716,152 + GFF1371 0.24 -1.3 716,153 - GFF1371 0.24 +0.3 716,215 + GFF1371 0.26 +0.3
Or see this region's nucleotide sequence