Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1324 and GFF1325 are separated by 5 nucleotides GFF1325 and GFF1326 are separated by 63 nucleotides GFF1326 and GFF1327 are separated by 139 nucleotides GFF1327 and GFF1328 overlap by 4 nucleotides GFF1328 and GFF1329 are separated by 10 nucleotides
GFF1324 - Phosphoribosylformylglycinamidine synthase, synthetase subunit (EC 6.3.5.3) / Phosphoribosylformylglycinamidine synthase, glutamine amidotransferase subunit (EC 6.3.5.3), at 662,221 to 666,189
GFF1324
GFF1325 - no description, at 666,195 to 666,506
GFF1325
GFF1326 - Peptidyl-prolyl cis-trans isomerase (EC 5.2.1.8), at 666,570 to 667,355
GFF1326
GFF1327 - Cell division protein BolA, at 667,495 to 667,788
GFF1327
GFF1328 - Intracellular septation protein IspA, at 667,785 to 668,327
GFF1328
GFF1329 - Peptide-methionine (R)-S-oxide reductase MsrB (EC 1.8.4.12), at 668,338 to 668,754
GFF1329
Position (kb)
666
667
668 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 665.599 kb on + strand, within GFF1324 at 665.600 kb on - strand, within GFF1324 at 665.999 kb on - strand at 665.999 kb on - strand at 666.346 kb on + strand, within GFF1325 at 666.346 kb on + strand, within GFF1325 at 666.347 kb on - strand, within GFF1325 at 666.347 kb on - strand, within GFF1325 at 666.347 kb on - strand, within GFF1325 at 666.390 kb on + strand, within GFF1325 at 666.390 kb on + strand, within GFF1325 at 666.390 kb on + strand, within GFF1325 at 666.390 kb on + strand, within GFF1325 at 666.390 kb on + strand, within GFF1325 at 666.390 kb on + strand, within GFF1325 at 666.390 kb on + strand, within GFF1325 at 666.390 kb on + strand, within GFF1325 at 666.390 kb on + strand, within GFF1325 at 666.390 kb on + strand, within GFF1325 at 666.390 kb on + strand, within GFF1325 at 666.390 kb on + strand, within GFF1325 at 666.391 kb on - strand, within GFF1325 at 666.391 kb on - strand, within GFF1325 at 666.391 kb on - strand, within GFF1325 at 666.391 kb on - strand, within GFF1325 at 666.391 kb on - strand, within GFF1325 at 666.572 kb on - strand at 666.607 kb on + strand at 666.607 kb on + strand at 666.607 kb on + strand at 666.607 kb on + strand at 666.607 kb on + strand at 666.607 kb on + strand at 666.955 kb on + strand, within GFF1326 at 666.955 kb on + strand, within GFF1326 at 666.955 kb on + strand, within GFF1326 at 666.955 kb on + strand, within GFF1326 at 666.955 kb on + strand, within GFF1326 at 666.955 kb on + strand, within GFF1326 at 666.955 kb on + strand, within GFF1326 at 666.955 kb on + strand, within GFF1326 at 666.955 kb on + strand, within GFF1326 at 666.955 kb on + strand, within GFF1326 at 666.956 kb on - strand, within GFF1326 at 666.956 kb on - strand, within GFF1326 at 666.956 kb on - strand, within GFF1326 at 666.956 kb on - strand, within GFF1326 at 666.956 kb on - strand, within GFF1326 at 666.956 kb on - strand, within GFF1326 at 666.956 kb on - strand, within GFF1326 at 666.956 kb on - strand, within GFF1326 at 666.991 kb on + strand, within GFF1326 at 666.992 kb on - strand, within GFF1326 at 667.036 kb on + strand, within GFF1326 at 667.036 kb on + strand, within GFF1326 at 667.036 kb on + strand, within GFF1326 at 667.036 kb on + strand, within GFF1326 at 667.036 kb on + strand, within GFF1326 at 667.037 kb on - strand, within GFF1326 at 667.037 kb on - strand, within GFF1326 at 667.037 kb on - strand, within GFF1326 at 667.037 kb on - strand, within GFF1326 at 667.037 kb on - strand, within GFF1326 at 667.037 kb on - strand, within GFF1326 at 667.037 kb on - strand, within GFF1326 at 667.037 kb on - strand, within GFF1326 at 667.037 kb on - strand, within GFF1326 at 667.037 kb on - strand, within GFF1326 at 667.096 kb on + strand, within GFF1326 at 667.096 kb on + strand, within GFF1326 at 667.096 kb on + strand, within GFF1326 at 667.097 kb on - strand, within GFF1326 at 667.097 kb on - strand, within GFF1326 at 667.097 kb on - strand, within GFF1326 at 667.097 kb on - strand, within GFF1326 at 667.364 kb on + strand at 667.365 kb on - strand at 667.391 kb on + strand at 667.391 kb on + strand at 667.391 kb on + strand at 667.391 kb on + strand at 667.391 kb on + strand at 667.392 kb on - strand at 667.392 kb on - strand at 667.556 kb on + strand, within GFF1327 at 667.556 kb on + strand, within GFF1327 at 667.556 kb on + strand, within GFF1327 at 667.556 kb on + strand, within GFF1327 at 667.556 kb on + strand, within GFF1327 at 667.556 kb on + strand, within GFF1327 at 667.556 kb on + strand, within GFF1327 at 667.556 kb on + strand, within GFF1327 at 667.556 kb on + strand, within GFF1327 at 667.556 kb on + strand, within GFF1327 at 667.556 kb on + strand, within GFF1327 at 667.556 kb on + strand, within GFF1327 at 667.556 kb on + strand, within GFF1327 at 667.556 kb on + strand, within GFF1327 at 667.556 kb on + strand, within GFF1327 at 667.556 kb on + strand, within GFF1327 at 667.556 kb on + strand, within GFF1327 at 667.556 kb on + strand, within GFF1327 at 667.557 kb on - strand, within GFF1327 at 667.557 kb on - strand, within GFF1327 at 667.557 kb on - strand, within GFF1327 at 667.557 kb on - strand, within GFF1327 at 667.557 kb on - strand, within GFF1327 at 667.557 kb on - strand, within GFF1327 at 667.557 kb on - strand, within GFF1327 at 667.557 kb on - strand, within GFF1327 at 667.557 kb on - strand, within GFF1327 at 667.557 kb on - strand, within GFF1327 at 667.557 kb on - strand, within GFF1327 at 667.557 kb on - strand, within GFF1327 at 667.557 kb on - strand, within GFF1327 at 667.557 kb on - strand, within GFF1327 at 667.557 kb on - strand, within GFF1327 at 667.767 kb on + strand at 667.768 kb on - strand at 667.773 kb on + strand at 667.774 kb on - strand at 667.774 kb on - strand at 667.812 kb on - strand at 667.828 kb on + strand at 667.828 kb on + strand at 667.828 kb on + strand at 667.828 kb on + strand at 667.828 kb on + strand at 667.828 kb on + strand at 667.828 kb on + strand at 667.828 kb on + strand at 667.829 kb on - strand at 667.829 kb on - strand at 667.829 kb on - strand at 667.829 kb on - strand at 667.829 kb on - strand at 667.829 kb on - strand at 667.829 kb on - strand at 667.829 kb on - strand at 667.829 kb on - strand at 667.829 kb on - strand at 667.829 kb on - strand at 667.829 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 665,599 + GFF1324 0.85 -0.3 665,600 - GFF1324 0.85 -0.3 665,999 - +0.3 665,999 - -1.7 666,346 + GFF1325 0.48 +0.4 666,346 + GFF1325 0.48 -2.1 666,347 - GFF1325 0.49 -0.5 666,347 - GFF1325 0.49 -0.3 666,347 - GFF1325 0.49 +0.2 666,390 + GFF1325 0.62 -1.1 666,390 + GFF1325 0.62 -0.1 666,390 + GFF1325 0.62 +1.9 666,390 + GFF1325 0.62 -0.2 666,390 + GFF1325 0.62 +0.4 666,390 + GFF1325 0.62 +0.5 666,390 + GFF1325 0.62 +0.0 666,390 + GFF1325 0.62 +0.1 666,390 + GFF1325 0.62 +0.6 666,390 + GFF1325 0.62 +0.2 666,390 + GFF1325 0.62 -2.3 666,390 + GFF1325 0.62 +1.3 666,391 - GFF1325 0.63 +0.6 666,391 - GFF1325 0.63 +0.6 666,391 - GFF1325 0.63 -0.4 666,391 - GFF1325 0.63 +0.3 666,391 - GFF1325 0.63 +0.0 666,572 - +0.1 666,607 + +0.1 666,607 + -0.3 666,607 + -1.4 666,607 + -0.3 666,607 + +0.9 666,607 + +1.5 666,955 + GFF1326 0.49 -1.7 666,955 + GFF1326 0.49 -1.5 666,955 + GFF1326 0.49 +1.7 666,955 + GFF1326 0.49 -1.3 666,955 + GFF1326 0.49 -0.8 666,955 + GFF1326 0.49 -0.8 666,955 + GFF1326 0.49 -0.5 666,955 + GFF1326 0.49 -0.8 666,955 + GFF1326 0.49 +1.9 666,955 + GFF1326 0.49 +0.5 666,956 - GFF1326 0.49 +0.5 666,956 - GFF1326 0.49 -1.5 666,956 - GFF1326 0.49 -1.7 666,956 - GFF1326 0.49 -0.3 666,956 - GFF1326 0.49 -0.4 666,956 - GFF1326 0.49 -0.4 666,956 - GFF1326 0.49 +1.3 666,956 - GFF1326 0.49 -1.0 666,991 + GFF1326 0.54 -1.0 666,992 - GFF1326 0.54 +1.3 667,036 + GFF1326 0.59 -0.9 667,036 + GFF1326 0.59 -1.2 667,036 + GFF1326 0.59 -0.4 667,036 + GFF1326 0.59 -0.1 667,036 + GFF1326 0.59 -1.1 667,037 - GFF1326 0.59 +0.3 667,037 - GFF1326 0.59 -0.5 667,037 - GFF1326 0.59 -0.8 667,037 - GFF1326 0.59 +1.3 667,037 - GFF1326 0.59 -0.4 667,037 - GFF1326 0.59 -0.2 667,037 - GFF1326 0.59 +0.7 667,037 - GFF1326 0.59 -0.5 667,037 - GFF1326 0.59 -1.1 667,037 - GFF1326 0.59 -0.2 667,096 + GFF1326 0.67 -0.2 667,096 + GFF1326 0.67 +1.2 667,096 + GFF1326 0.67 -0.1 667,097 - GFF1326 0.67 -0.0 667,097 - GFF1326 0.67 +0.2 667,097 - GFF1326 0.67 -1.3 667,097 - GFF1326 0.67 -0.4 667,364 + -0.5 667,365 - +0.5 667,391 + -0.3 667,391 + +2.7 667,391 + -1.1 667,391 + -0.8 667,391 + +0.0 667,392 - -0.6 667,392 - +0.8 667,556 + GFF1327 0.21 -2.1 667,556 + GFF1327 0.21 -0.3 667,556 + GFF1327 0.21 +0.3 667,556 + GFF1327 0.21 -0.5 667,556 + GFF1327 0.21 +0.8 667,556 + GFF1327 0.21 +1.3 667,556 + GFF1327 0.21 -0.0 667,556 + GFF1327 0.21 +0.1 667,556 + GFF1327 0.21 -0.5 667,556 + GFF1327 0.21 -0.6 667,556 + GFF1327 0.21 -0.3 667,556 + GFF1327 0.21 -0.5 667,556 + GFF1327 0.21 -0.7 667,556 + GFF1327 0.21 -0.1 667,556 + GFF1327 0.21 -2.9 667,556 + GFF1327 0.21 +0.5 667,556 + GFF1327 0.21 -1.1 667,556 + GFF1327 0.21 +0.5 667,557 - GFF1327 0.21 -0.7 667,557 - GFF1327 0.21 -0.1 667,557 - GFF1327 0.21 -0.6 667,557 - GFF1327 0.21 -2.7 667,557 - GFF1327 0.21 -0.5 667,557 - GFF1327 0.21 +0.4 667,557 - GFF1327 0.21 +0.2 667,557 - GFF1327 0.21 +0.1 667,557 - GFF1327 0.21 -0.5 667,557 - GFF1327 0.21 -1.1 667,557 - GFF1327 0.21 -2.8 667,557 - GFF1327 0.21 -0.8 667,557 - GFF1327 0.21 -0.3 667,557 - GFF1327 0.21 -0.2 667,557 - GFF1327 0.21 -0.4 667,767 + +0.5 667,768 - -3.2 667,773 + -0.8 667,774 - -2.5 667,774 - -0.6 667,812 - -1.6 667,828 + -0.1 667,828 + +0.4 667,828 + -0.7 667,828 + -0.5 667,828 + -0.1 667,828 + +0.5 667,828 + -0.5 667,828 + -2.6 667,829 - +1.8 667,829 - +0.6 667,829 - +1.3 667,829 - +0.5 667,829 - -0.8 667,829 - -0.3 667,829 - -0.6 667,829 - +1.5 667,829 - +0.1 667,829 - +0.6 667,829 - -0.1 667,829 - -0.9
Or see this region's nucleotide sequence