Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1153 and GFF1154 overlap by 4 nucleotides GFF1154 and GFF1155 are separated by 14 nucleotides GFF1155 and GFF1156 are separated by 16 nucleotides GFF1156 and GFF1157 overlap by 4 nucleotides
GFF1153 - Diacylglycerol kinase (EC 2.7.1.107), at 470,177 to 470,584
GFF1153
GFF1154 - Putative transmembrane protein, at 470,581 to 471,147
GFF1154
GFF1155 - Putative transmembrane protein, at 471,162 to 471,986
GFF1155
GFF1156 - Putative transmembrane protein, at 472,003 to 472,425
GFF1156
GFF1157 - DNA-directed RNA polymerase specialized sigma subunit, sigma24-like, at 472,422 to 472,988
GFF1157
Position (kb)
471
472 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 470.783 kb on + strand, within GFF1154 at 470.840 kb on - strand, within GFF1154 at 470.841 kb on + strand, within GFF1154 at 470.841 kb on + strand, within GFF1154 at 470.841 kb on + strand, within GFF1154 at 470.842 kb on - strand, within GFF1154 at 470.842 kb on - strand, within GFF1154 at 470.872 kb on + strand, within GFF1154 at 470.894 kb on + strand, within GFF1154 at 470.894 kb on + strand, within GFF1154 at 470.894 kb on + strand, within GFF1154 at 470.894 kb on + strand, within GFF1154 at 470.894 kb on + strand, within GFF1154 at 470.894 kb on + strand, within GFF1154 at 470.894 kb on + strand, within GFF1154 at 470.895 kb on - strand, within GFF1154 at 470.895 kb on - strand, within GFF1154 at 470.895 kb on - strand, within GFF1154 at 470.895 kb on - strand, within GFF1154 at 470.909 kb on - strand, within GFF1154 at 471.026 kb on + strand, within GFF1154 at 471.026 kb on + strand, within GFF1154 at 471.026 kb on + strand, within GFF1154 at 471.026 kb on + strand, within GFF1154 at 471.026 kb on + strand, within GFF1154 at 471.026 kb on + strand, within GFF1154 at 471.026 kb on + strand, within GFF1154 at 471.026 kb on + strand, within GFF1154 at 471.027 kb on - strand, within GFF1154 at 471.027 kb on - strand, within GFF1154 at 471.027 kb on - strand, within GFF1154 at 471.027 kb on - strand, within GFF1154 at 471.027 kb on - strand, within GFF1154 at 471.027 kb on - strand, within GFF1154 at 471.210 kb on + strand at 471.210 kb on + strand at 471.211 kb on - strand at 471.211 kb on - strand at 471.211 kb on - strand at 471.211 kb on - strand at 471.335 kb on + strand, within GFF1155 at 471.526 kb on + strand, within GFF1155 at 471.526 kb on + strand, within GFF1155 at 471.526 kb on + strand, within GFF1155 at 471.527 kb on - strand, within GFF1155 at 471.527 kb on - strand, within GFF1155 at 471.527 kb on - strand, within GFF1155 at 471.527 kb on - strand, within GFF1155 at 471.527 kb on - strand, within GFF1155 at 471.679 kb on + strand, within GFF1155 at 471.679 kb on + strand, within GFF1155 at 471.679 kb on + strand, within GFF1155 at 471.680 kb on - strand, within GFF1155 at 471.680 kb on - strand, within GFF1155 at 471.680 kb on - strand, within GFF1155 at 471.680 kb on - strand, within GFF1155 at 471.956 kb on + strand at 471.956 kb on + strand at 471.956 kb on + strand at 471.956 kb on + strand at 471.956 kb on + strand at 471.956 kb on + strand at 471.956 kb on + strand at 471.957 kb on - strand at 471.957 kb on - strand at 471.957 kb on - strand at 471.957 kb on - strand at 472.053 kb on + strand, within GFF1156 at 472.054 kb on - strand, within GFF1156 at 472.054 kb on - strand, within GFF1156 at 472.135 kb on + strand, within GFF1156 at 472.135 kb on + strand, within GFF1156 at 472.135 kb on + strand, within GFF1156 at 472.136 kb on - strand, within GFF1156 at 472.136 kb on - strand, within GFF1156 at 472.136 kb on - strand, within GFF1156 at 472.416 kb on + strand at 472.417 kb on - strand at 472.417 kb on - strand at 472.417 kb on - strand at 472.417 kb on - strand at 472.417 kb on - strand at 472.417 kb on - strand at 472.417 kb on - strand at 472.417 kb on - strand at 472.417 kb on - strand at 472.417 kb on - strand at 472.419 kb on + strand at 472.419 kb on + strand at 472.419 kb on + strand at 472.419 kb on + strand at 472.419 kb on + strand at 472.420 kb on - strand at 472.420 kb on - strand at 472.420 kb on - strand at 472.420 kb on - strand at 472.420 kb on - strand at 472.420 kb on - strand at 472.425 kb on + strand at 472.425 kb on + strand at 472.425 kb on + strand at 472.431 kb on + strand at 472.431 kb on + strand at 472.553 kb on - strand, within GFF1157 at 472.553 kb on - strand, within GFF1157 at 472.664 kb on + strand, within GFF1157 at 472.665 kb on - strand, within GFF1157 at 472.849 kb on + strand, within GFF1157 at 472.849 kb on + strand, within GFF1157 at 472.849 kb on + strand, within GFF1157 at 472.850 kb on - strand, within GFF1157 at 472.886 kb on + strand, within GFF1157 at 472.918 kb on + strand, within GFF1157 at 472.918 kb on + strand, within GFF1157 at 472.918 kb on + strand, within GFF1157 at 472.918 kb on + strand, within GFF1157 at 472.919 kb on - strand, within GFF1157 at 472.974 kb on + strand at 472.974 kb on + strand at 472.974 kb on + strand at 472.974 kb on + strand at 472.974 kb on + strand at 472.974 kb on + strand at 472.975 kb on - strand at 472.975 kb on - strand at 472.975 kb on - strand at 472.975 kb on - strand at 472.975 kb on - strand at 472.975 kb on - strand at 472.975 kb on - strand at 472.975 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 470,783 + GFF1154 0.36 -1.1 470,840 - GFF1154 0.46 -0.1 470,841 + GFF1154 0.46 -3.2 470,841 + GFF1154 0.46 -2.0 470,841 + GFF1154 0.46 -0.4 470,842 - GFF1154 0.46 +1.4 470,842 - GFF1154 0.46 +0.5 470,872 + GFF1154 0.51 -2.0 470,894 + GFF1154 0.55 -1.0 470,894 + GFF1154 0.55 -0.3 470,894 + GFF1154 0.55 -0.5 470,894 + GFF1154 0.55 -1.1 470,894 + GFF1154 0.55 -1.6 470,894 + GFF1154 0.55 -2.5 470,894 + GFF1154 0.55 -1.0 470,895 - GFF1154 0.55 -0.6 470,895 - GFF1154 0.55 -0.5 470,895 - GFF1154 0.55 -0.5 470,895 - GFF1154 0.55 -1.1 470,909 - GFF1154 0.58 -1.1 471,026 + GFF1154 0.78 -0.6 471,026 + GFF1154 0.78 -0.6 471,026 + GFF1154 0.78 -1.5 471,026 + GFF1154 0.78 +0.6 471,026 + GFF1154 0.78 -0.2 471,026 + GFF1154 0.78 -1.9 471,026 + GFF1154 0.78 -1.0 471,026 + GFF1154 0.78 -0.4 471,027 - GFF1154 0.79 -1.8 471,027 - GFF1154 0.79 +0.6 471,027 - GFF1154 0.79 -0.7 471,027 - GFF1154 0.79 +0.4 471,027 - GFF1154 0.79 -1.0 471,027 - GFF1154 0.79 +0.7 471,210 + -0.4 471,210 + +1.1 471,211 - -0.7 471,211 - -0.6 471,211 - +0.4 471,211 - +0.2 471,335 + GFF1155 0.21 +0.9 471,526 + GFF1155 0.44 -1.7 471,526 + GFF1155 0.44 -0.0 471,526 + GFF1155 0.44 -1.1 471,527 - GFF1155 0.44 -1.1 471,527 - GFF1155 0.44 -1.2 471,527 - GFF1155 0.44 -1.2 471,527 - GFF1155 0.44 -2.2 471,527 - GFF1155 0.44 -1.1 471,679 + GFF1155 0.63 -1.8 471,679 + GFF1155 0.63 +1.4 471,679 + GFF1155 0.63 -1.3 471,680 - GFF1155 0.63 -1.0 471,680 - GFF1155 0.63 -1.2 471,680 - GFF1155 0.63 -0.2 471,680 - GFF1155 0.63 +0.4 471,956 + -1.5 471,956 + -0.2 471,956 + -0.4 471,956 + -0.8 471,956 + -0.1 471,956 + -0.9 471,956 + -0.2 471,957 - -1.7 471,957 - -0.2 471,957 - -0.1 471,957 - -0.6 472,053 + GFF1156 0.12 -1.6 472,054 - GFF1156 0.12 -0.4 472,054 - GFF1156 0.12 +0.4 472,135 + GFF1156 0.31 +0.5 472,135 + GFF1156 0.31 -0.6 472,135 + GFF1156 0.31 -1.0 472,136 - GFF1156 0.31 +0.4 472,136 - GFF1156 0.31 -1.2 472,136 - GFF1156 0.31 +0.0 472,416 + +0.0 472,417 - -2.2 472,417 - -0.5 472,417 - -1.2 472,417 - +0.3 472,417 - -2.1 472,417 - -0.2 472,417 - +0.0 472,417 - -2.5 472,417 - -2.0 472,417 - -1.5 472,419 + -1.0 472,419 + +0.4 472,419 + -0.1 472,419 + -2.2 472,419 + -0.7 472,420 - +1.0 472,420 - -1.0 472,420 - +0.5 472,420 - -0.3 472,420 - -0.5 472,420 - -1.6 472,425 + -1.1 472,425 + -0.7 472,425 + +0.8 472,431 + +0.5 472,431 + -1.0 472,553 - GFF1157 0.23 -0.5 472,553 - GFF1157 0.23 +0.1 472,664 + GFF1157 0.43 -0.4 472,665 - GFF1157 0.43 +0.0 472,849 + GFF1157 0.75 +0.4 472,849 + GFF1157 0.75 +0.5 472,849 + GFF1157 0.75 +0.5 472,850 - GFF1157 0.75 +0.2 472,886 + GFF1157 0.82 -0.6 472,918 + GFF1157 0.87 -0.4 472,918 + GFF1157 0.87 -0.3 472,918 + GFF1157 0.87 -0.5 472,918 + GFF1157 0.87 +0.0 472,919 - GFF1157 0.88 -0.1 472,974 + -1.6 472,974 + -0.9 472,974 + -0.4 472,974 + -0.2 472,974 + -0.2 472,974 + -0.5 472,975 - -0.6 472,975 - +0.4 472,975 - +0.6 472,975 - -0.2 472,975 - +0.3 472,975 - -0.1 472,975 - -0.6 472,975 - -0.4
Or see this region's nucleotide sequence