Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1087 and GFF1088 are separated by 145 nucleotides GFF1088 and GFF1089 are separated by 179 nucleotides
GFF1087 - Thioredoxin reductase (EC 1.8.1.9), at 397,475 to 399,154
GFF1087
GFF1088 - Putative hemolysin, at 399,300 to 400,184
GFF1088
GFF1089 - Polyphosphate kinase (EC 2.7.4.1), at 400,364 to 402,502
GFF1089
Position (kb) 399
400
401 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 398.336 kb on + strand, within GFF1087 at 398.466 kb on + strand, within GFF1087 at 398.466 kb on + strand, within GFF1087 at 398.467 kb on - strand, within GFF1087 at 398.467 kb on - strand, within GFF1087 at 398.467 kb on - strand, within GFF1087 at 398.528 kb on - strand, within GFF1087 at 398.584 kb on - strand, within GFF1087 at 398.584 kb on - strand, within GFF1087 at 398.638 kb on - strand, within GFF1087 at 398.644 kb on - strand, within GFF1087 at 398.810 kb on + strand, within GFF1087 at 398.810 kb on + strand, within GFF1087 at 398.811 kb on - strand, within GFF1087 at 399.189 kb on + strand at 399.522 kb on + strand, within GFF1088 at 399.522 kb on + strand, within GFF1088 at 399.655 kb on - strand, within GFF1088 at 399.655 kb on - strand, within GFF1088 at 399.655 kb on - strand, within GFF1088 at 399.700 kb on - strand, within GFF1088 at 399.700 kb on - strand, within GFF1088 at 399.700 kb on - strand, within GFF1088 at 399.700 kb on - strand, within GFF1088 at 399.700 kb on - strand, within GFF1088 at 399.700 kb on - strand, within GFF1088 at 399.846 kb on - strand, within GFF1088 at 399.970 kb on - strand, within GFF1088 at 400.054 kb on - strand, within GFF1088 at 400.284 kb on + strand at 400.285 kb on - strand at 400.285 kb on - strand at 400.285 kb on - strand at 400.314 kb on + strand at 400.314 kb on + strand at 400.314 kb on + strand at 400.315 kb on - strand at 400.324 kb on + strand at 400.325 kb on - strand at 400.363 kb on + strand at 400.363 kb on + strand at 400.363 kb on + strand at 400.363 kb on + strand at 400.363 kb on + strand at 400.363 kb on + strand at 400.363 kb on + strand at 400.364 kb on - strand at 400.364 kb on - strand at 400.364 kb on - strand at 400.511 kb on + strand at 400.511 kb on + strand at 400.511 kb on + strand at 400.607 kb on + strand, within GFF1089 at 400.607 kb on + strand, within GFF1089 at 400.607 kb on + strand, within GFF1089 at 400.607 kb on + strand, within GFF1089 at 400.607 kb on + strand, within GFF1089 at 400.607 kb on + strand, within GFF1089 at 400.607 kb on + strand, within GFF1089 at 400.607 kb on + strand, within GFF1089 at 400.607 kb on + strand, within GFF1089 at 400.607 kb on + strand, within GFF1089 at 400.608 kb on - strand, within GFF1089 at 400.608 kb on - strand, within GFF1089 at 400.608 kb on - strand, within GFF1089 at 400.608 kb on - strand, within GFF1089 at 400.608 kb on - strand, within GFF1089 at 400.608 kb on - strand, within GFF1089 at 400.608 kb on - strand, within GFF1089 at 400.608 kb on - strand, within GFF1089 at 400.608 kb on - strand, within GFF1089 at 400.609 kb on + strand, within GFF1089 at 400.610 kb on - strand, within GFF1089 at 400.668 kb on - strand, within GFF1089 at 400.676 kb on + strand, within GFF1089 at 400.676 kb on + strand, within GFF1089 at 400.677 kb on - strand, within GFF1089 at 400.677 kb on - strand, within GFF1089 at 400.677 kb on - strand, within GFF1089 at 400.770 kb on + strand, within GFF1089 at 400.773 kb on - strand, within GFF1089 at 400.992 kb on + strand, within GFF1089 at 400.992 kb on + strand, within GFF1089 at 400.993 kb on - strand, within GFF1089 at 401.160 kb on - strand, within GFF1089 at 401.160 kb on - strand, within GFF1089
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 398,336 + GFF1087 0.51 -0.9 398,466 + GFF1087 0.59 -0.2 398,466 + GFF1087 0.59 +0.5 398,467 - GFF1087 0.59 +0.4 398,467 - GFF1087 0.59 -0.3 398,467 - GFF1087 0.59 +0.8 398,528 - GFF1087 0.63 +0.8 398,584 - GFF1087 0.66 -0.9 398,584 - GFF1087 0.66 -1.0 398,638 - GFF1087 0.69 +0.4 398,644 - GFF1087 0.70 +0.1 398,810 + GFF1087 0.79 +0.0 398,810 + GFF1087 0.79 +0.7 398,811 - GFF1087 0.80 -0.6 399,189 + +0.4 399,522 + GFF1088 0.25 +1.4 399,522 + GFF1088 0.25 +1.5 399,655 - GFF1088 0.40 -2.5 399,655 - GFF1088 0.40 +0.3 399,655 - GFF1088 0.40 -0.1 399,700 - GFF1088 0.45 -0.2 399,700 - GFF1088 0.45 +0.1 399,700 - GFF1088 0.45 -0.7 399,700 - GFF1088 0.45 -1.9 399,700 - GFF1088 0.45 -0.5 399,700 - GFF1088 0.45 -0.8 399,846 - GFF1088 0.62 +0.8 399,970 - GFF1088 0.76 +0.7 400,054 - GFF1088 0.85 -0.4 400,284 + -2.2 400,285 - +0.4 400,285 - +0.1 400,285 - +0.6 400,314 + -0.2 400,314 + -0.8 400,314 + -1.0 400,315 - -0.6 400,324 + -0.4 400,325 - -0.5 400,363 + -0.4 400,363 + -0.3 400,363 + -2.4 400,363 + -0.6 400,363 + -0.4 400,363 + -0.2 400,363 + -0.6 400,364 - -0.4 400,364 - +1.1 400,364 - -0.4 400,511 + -1.2 400,511 + +0.9 400,511 + +0.1 400,607 + GFF1089 0.11 -0.6 400,607 + GFF1089 0.11 -1.9 400,607 + GFF1089 0.11 +1.1 400,607 + GFF1089 0.11 -0.3 400,607 + GFF1089 0.11 -3.0 400,607 + GFF1089 0.11 -0.0 400,607 + GFF1089 0.11 -0.5 400,607 + GFF1089 0.11 -1.1 400,607 + GFF1089 0.11 -0.3 400,607 + GFF1089 0.11 -0.5 400,608 - GFF1089 0.11 +0.5 400,608 - GFF1089 0.11 -0.2 400,608 - GFF1089 0.11 -1.4 400,608 - GFF1089 0.11 -0.8 400,608 - GFF1089 0.11 +0.2 400,608 - GFF1089 0.11 -0.1 400,608 - GFF1089 0.11 -1.2 400,608 - GFF1089 0.11 -2.5 400,608 - GFF1089 0.11 +0.4 400,609 + GFF1089 0.11 -2.6 400,610 - GFF1089 0.12 -3.0 400,668 - GFF1089 0.14 +1.2 400,676 + GFF1089 0.15 -2.6 400,676 + GFF1089 0.15 -0.2 400,677 - GFF1089 0.15 -1.4 400,677 - GFF1089 0.15 -0.2 400,677 - GFF1089 0.15 +0.0 400,770 + GFF1089 0.19 -0.8 400,773 - GFF1089 0.19 +0.4 400,992 + GFF1089 0.29 -1.1 400,992 + GFF1089 0.29 -0.4 400,993 - GFF1089 0.29 +0.3 401,160 - GFF1089 0.37 +2.4 401,160 - GFF1089 0.37 -0.2
Or see this region's nucleotide sequence