Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF880 and GFF881 are separated by 47 nucleotides GFF881 and GFF882 overlap by 13 nucleotides GFF882 and GFF883 are separated by 144 nucleotides
GFF880 - conserved hypothetical protein, at 189,351 to 190,094
GFF880
GFF881 - Transcriptional regulator ligR, LysR family, at 190,142 to 191,065
GFF881
GFF882 - Outer membrane porin, at 191,053 to 192,117
GFF882
GFF883 - 2-pyrone-4,6-dicarboxylic acid hydrolase (EC 3.1.1.57), at 192,262 to 193,137
GFF883
Position (kb) 191
192
193 Strain fitness (log2 ratio) -2
-1
0
1
2 at 190.074 kb on - strand at 190.102 kb on - strand at 190.102 kb on - strand at 190.163 kb on + strand at 190.163 kb on + strand at 190.164 kb on - strand at 190.164 kb on - strand at 190.164 kb on - strand at 190.164 kb on - strand at 190.580 kb on + strand, within GFF881 at 190.580 kb on + strand, within GFF881 at 190.581 kb on - strand, within GFF881 at 191.034 kb on - strand at 191.034 kb on - strand at 191.034 kb on - strand at 191.123 kb on + strand at 191.123 kb on + strand at 191.166 kb on - strand, within GFF882 at 191.336 kb on + strand, within GFF882 at 191.528 kb on + strand, within GFF882 at 191.529 kb on - strand, within GFF882 at 191.570 kb on + strand, within GFF882 at 191.570 kb on + strand, within GFF882 at 191.570 kb on + strand, within GFF882 at 191.571 kb on - strand, within GFF882 at 191.571 kb on - strand, within GFF882 at 191.618 kb on + strand, within GFF882 at 191.618 kb on + strand, within GFF882 at 191.777 kb on + strand, within GFF882 at 191.777 kb on + strand, within GFF882 at 191.778 kb on - strand, within GFF882 at 191.892 kb on - strand, within GFF882 at 191.984 kb on + strand, within GFF882 at 192.146 kb on - strand at 192.146 kb on - strand at 192.233 kb on + strand at 192.275 kb on + strand at 192.275 kb on + strand at 192.467 kb on + strand, within GFF883 at 192.467 kb on + strand, within GFF883 at 192.468 kb on - strand, within GFF883 at 192.468 kb on - strand, within GFF883 at 192.468 kb on - strand, within GFF883 at 192.468 kb on - strand, within GFF883 at 192.656 kb on + strand, within GFF883 at 192.657 kb on - strand, within GFF883 at 192.657 kb on - strand, within GFF883 at 193.008 kb on - strand, within GFF883
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 190,074 - +0.2 190,102 - -0.5 190,102 - -0.3 190,163 + -1.0 190,163 + +1.9 190,164 - -0.2 190,164 - +0.1 190,164 - -0.9 190,164 - -0.1 190,580 + GFF881 0.47 -0.2 190,580 + GFF881 0.47 -0.5 190,581 - GFF881 0.48 -0.9 191,034 - -0.6 191,034 - +1.1 191,034 - -2.3 191,123 + -0.9 191,123 + -0.8 191,166 - GFF882 0.11 -1.1 191,336 + GFF882 0.27 -0.5 191,528 + GFF882 0.45 -0.7 191,529 - GFF882 0.45 +0.2 191,570 + GFF882 0.49 -0.3 191,570 + GFF882 0.49 -0.4 191,570 + GFF882 0.49 -0.4 191,571 - GFF882 0.49 +1.4 191,571 - GFF882 0.49 +1.4 191,618 + GFF882 0.53 -2.4 191,618 + GFF882 0.53 -0.3 191,777 + GFF882 0.68 -0.6 191,777 + GFF882 0.68 -0.1 191,778 - GFF882 0.68 -0.9 191,892 - GFF882 0.79 -0.2 191,984 + GFF882 0.87 +0.4 192,146 - -1.7 192,146 - -0.9 192,233 + -0.2 192,275 + +1.8 192,275 + +0.8 192,467 + GFF883 0.23 +0.6 192,467 + GFF883 0.23 -0.5 192,468 - GFF883 0.24 +1.2 192,468 - GFF883 0.24 -0.5 192,468 - GFF883 0.24 -0.5 192,468 - GFF883 0.24 +0.4 192,656 + GFF883 0.45 -0.2 192,657 - GFF883 0.45 -0.5 192,657 - GFF883 0.45 -0.4 193,008 - GFF883 0.85 +0.6
Or see this region's nucleotide sequence