Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF81 and GFF82 are separated by 8 nucleotides GFF82 and GFF83 overlap by 4 nucleotides GFF83 and GFF84 are separated by 112 nucleotides GFF84 and GFF85 are separated by 131 nucleotides
GFF81 - Bll6900 protein, at 98,293 to 98,895
GFF81
GFF82 - Alkylhydroperoxidase AhpD domain protein, at 98,904 to 99,557
GFF82
GFF83 - Alkanal monooxygenase alpha chain (EC 1.14.14.3), at 99,554 to 100,615
GFF83
GFF84 - Oxidoreductase, short-chain dehydrogenase/reductase family, at 100,728 to 101,444
GFF84
GFF85 - Transcriptional regulator, LysR family, at 101,576 to 102,475
GFF85
Position (kb) 99
100
101 Strain fitness (log2 ratio) -2
-1
0
1 at 98.582 kb on + strand, within GFF81 at 98.582 kb on + strand, within GFF81 at 98.582 kb on + strand, within GFF81 at 98.660 kb on + strand, within GFF81 at 98.660 kb on + strand, within GFF81 at 98.660 kb on + strand, within GFF81 at 98.661 kb on - strand, within GFF81 at 98.661 kb on - strand, within GFF81 at 98.661 kb on - strand, within GFF81 at 98.729 kb on + strand, within GFF81 at 98.730 kb on - strand, within GFF81 at 98.867 kb on + strand at 98.867 kb on + strand at 98.868 kb on - strand at 99.151 kb on + strand, within GFF82 at 99.152 kb on - strand, within GFF82 at 99.152 kb on - strand, within GFF82 at 99.152 kb on - strand, within GFF82 at 99.196 kb on + strand, within GFF82 at 99.196 kb on + strand, within GFF82 at 99.196 kb on + strand, within GFF82 at 99.196 kb on + strand, within GFF82 at 99.197 kb on - strand, within GFF82 at 99.295 kb on + strand, within GFF82 at 99.295 kb on + strand, within GFF82 at 99.295 kb on + strand, within GFF82 at 99.296 kb on - strand, within GFF82 at 99.296 kb on - strand, within GFF82 at 99.296 kb on - strand, within GFF82 at 99.412 kb on + strand, within GFF82 at 99.412 kb on + strand, within GFF82 at 99.412 kb on + strand, within GFF82 at 99.412 kb on + strand, within GFF82 at 99.413 kb on - strand, within GFF82 at 99.413 kb on - strand, within GFF82 at 99.413 kb on - strand, within GFF82 at 99.413 kb on - strand, within GFF82 at 99.960 kb on + strand, within GFF83 at 100.134 kb on + strand, within GFF83 at 100.135 kb on - strand, within GFF83 at 100.404 kb on + strand, within GFF83 at 100.404 kb on + strand, within GFF83 at 100.404 kb on + strand, within GFF83 at 100.404 kb on + strand, within GFF83 at 100.404 kb on + strand, within GFF83 at 100.404 kb on + strand, within GFF83 at 100.405 kb on - strand, within GFF83 at 100.405 kb on - strand, within GFF83 at 100.405 kb on - strand, within GFF83 at 100.695 kb on + strand at 100.695 kb on + strand at 100.695 kb on + strand at 100.695 kb on + strand at 100.696 kb on - strand at 100.757 kb on - strand at 101.110 kb on + strand, within GFF84 at 101.110 kb on + strand, within GFF84 at 101.111 kb on - strand, within GFF84 at 101.420 kb on - strand at 101.470 kb on + strand at 101.470 kb on + strand at 101.471 kb on - strand at 101.486 kb on + strand at 101.486 kb on + strand at 101.487 kb on - strand at 101.487 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 98,582 + GFF81 0.48 +1.2 98,582 + GFF81 0.48 -1.2 98,582 + GFF81 0.48 -0.2 98,660 + GFF81 0.61 -1.0 98,660 + GFF81 0.61 -2.2 98,660 + GFF81 0.61 +0.2 98,661 - GFF81 0.61 +0.9 98,661 - GFF81 0.61 +0.4 98,661 - GFF81 0.61 +0.5 98,729 + GFF81 0.72 -0.8 98,730 - GFF81 0.72 -0.4 98,867 + -1.9 98,867 + +1.2 98,868 - +1.2 99,151 + GFF82 0.38 -0.8 99,152 - GFF82 0.38 -0.1 99,152 - GFF82 0.38 +0.4 99,152 - GFF82 0.38 -0.3 99,196 + GFF82 0.45 +1.5 99,196 + GFF82 0.45 +0.0 99,196 + GFF82 0.45 +0.5 99,196 + GFF82 0.45 +0.1 99,197 - GFF82 0.45 +0.2 99,295 + GFF82 0.60 +0.3 99,295 + GFF82 0.60 +0.2 99,295 + GFF82 0.60 -0.3 99,296 - GFF82 0.60 -1.8 99,296 - GFF82 0.60 +0.8 99,296 - GFF82 0.60 +0.0 99,412 + GFF82 0.78 +0.1 99,412 + GFF82 0.78 -1.3 99,412 + GFF82 0.78 -0.7 99,412 + GFF82 0.78 -1.1 99,413 - GFF82 0.78 -1.1 99,413 - GFF82 0.78 -1.1 99,413 - GFF82 0.78 -0.3 99,413 - GFF82 0.78 +0.6 99,960 + GFF83 0.38 -0.7 100,134 + GFF83 0.55 -0.2 100,135 - GFF83 0.55 -0.2 100,404 + GFF83 0.80 +0.2 100,404 + GFF83 0.80 -0.4 100,404 + GFF83 0.80 -1.2 100,404 + GFF83 0.80 -0.9 100,404 + GFF83 0.80 +0.4 100,404 + GFF83 0.80 -0.9 100,405 - GFF83 0.80 +0.1 100,405 - GFF83 0.80 -0.5 100,405 - GFF83 0.80 -2.1 100,695 + +0.0 100,695 + -0.4 100,695 + -0.9 100,695 + -0.6 100,696 - +1.2 100,757 - -1.3 101,110 + GFF84 0.53 -0.8 101,110 + GFF84 0.53 +0.3 101,111 - GFF84 0.53 -0.8 101,420 - -0.9 101,470 + +0.1 101,470 + +0.4 101,471 - -0.4 101,486 + -0.2 101,486 + -0.1 101,487 - +0.2 101,487 - +0.7
Or see this region's nucleotide sequence