Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF818 and GFF819 are separated by 4 nucleotides GFF819 and GFF820 overlap by 4 nucleotides GFF820 and GFF821 are separated by 203 nucleotides
GFF818 - BUG/TctC family periplasmic protein, at 123,392 to 124,402
GFF818
GFF819 - Uncharacterized protein CC_2099, at 124,407 to 124,952
GFF819
GFF820 - Intracellular protease, at 124,949 to 125,596
GFF820
GFF821 - Acetoacetyl-CoA reductase (EC 1.1.1.36), at 125,800 to 126,537
GFF821
Position (kb)
124
125
126 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 123.968 kb on + strand, within GFF818 at 123.969 kb on - strand, within GFF818 at 124.184 kb on + strand, within GFF818 at 124.184 kb on + strand, within GFF818 at 124.184 kb on + strand, within GFF818 at 124.184 kb on + strand, within GFF818 at 124.184 kb on + strand, within GFF818 at 124.184 kb on + strand, within GFF818 at 124.184 kb on + strand, within GFF818 at 124.184 kb on + strand, within GFF818 at 124.184 kb on + strand, within GFF818 at 124.184 kb on + strand, within GFF818 at 124.184 kb on + strand, within GFF818 at 124.185 kb on - strand, within GFF818 at 124.185 kb on - strand, within GFF818 at 124.185 kb on - strand, within GFF818 at 124.185 kb on - strand, within GFF818 at 124.185 kb on - strand, within GFF818 at 124.185 kb on - strand, within GFF818 at 124.185 kb on - strand, within GFF818 at 124.185 kb on - strand, within GFF818 at 124.185 kb on - strand, within GFF818 at 124.185 kb on - strand, within GFF818 at 124.185 kb on - strand, within GFF818 at 124.185 kb on - strand, within GFF818 at 124.585 kb on + strand, within GFF819 at 124.756 kb on + strand, within GFF819 at 124.757 kb on - strand, within GFF819 at 124.757 kb on - strand, within GFF819 at 124.987 kb on - strand at 124.987 kb on - strand at 124.987 kb on - strand at 124.987 kb on - strand at 125.022 kb on + strand, within GFF820 at 125.040 kb on + strand, within GFF820 at 125.040 kb on + strand, within GFF820 at 125.041 kb on - strand, within GFF820 at 125.041 kb on - strand, within GFF820 at 125.041 kb on - strand, within GFF820 at 125.043 kb on + strand, within GFF820 at 125.044 kb on - strand, within GFF820 at 125.044 kb on - strand, within GFF820 at 125.044 kb on - strand, within GFF820 at 125.394 kb on + strand, within GFF820 at 125.394 kb on + strand, within GFF820 at 125.394 kb on + strand, within GFF820 at 125.394 kb on + strand, within GFF820 at 125.394 kb on + strand, within GFF820 at 125.394 kb on + strand, within GFF820 at 125.395 kb on - strand, within GFF820 at 125.395 kb on - strand, within GFF820 at 125.395 kb on - strand, within GFF820 at 125.597 kb on - strand at 125.647 kb on + strand at 125.647 kb on + strand at 125.647 kb on + strand at 125.647 kb on + strand at 125.647 kb on + strand at 125.647 kb on + strand at 125.648 kb on - strand at 125.648 kb on - strand at 125.661 kb on + strand at 125.661 kb on + strand at 125.662 kb on - strand at 125.662 kb on - strand at 125.662 kb on - strand at 125.730 kb on + strand at 125.750 kb on - strand at 125.750 kb on - strand at 125.779 kb on + strand at 125.780 kb on - strand at 125.780 kb on - strand at 125.780 kb on - strand at 125.782 kb on + strand at 125.783 kb on + strand at 125.783 kb on + strand at 125.783 kb on + strand at 125.783 kb on + strand at 125.783 kb on + strand at 125.783 kb on + strand at 125.783 kb on + strand at 125.784 kb on - strand at 125.784 kb on - strand at 125.784 kb on - strand at 125.784 kb on - strand at 125.784 kb on - strand at 125.784 kb on - strand at 125.784 kb on - strand at 125.787 kb on + strand at 125.787 kb on + strand at 125.788 kb on - strand at 125.788 kb on - strand at 125.788 kb on - strand at 125.818 kb on + strand at 125.818 kb on + strand at 125.818 kb on + strand at 125.818 kb on + strand at 125.818 kb on + strand at 125.818 kb on + strand at 125.818 kb on + strand at 125.818 kb on + strand at 125.818 kb on + strand at 125.818 kb on + strand at 125.819 kb on - strand at 125.819 kb on - strand at 125.819 kb on - strand at 125.819 kb on - strand at 125.819 kb on - strand at 125.917 kb on + strand, within GFF821 at 126.253 kb on + strand, within GFF821 at 126.253 kb on + strand, within GFF821 at 126.253 kb on + strand, within GFF821 at 126.254 kb on - strand, within GFF821 at 126.254 kb on - strand, within GFF821 at 126.350 kb on - strand, within GFF821 at 126.487 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 123,968 + GFF818 0.57 +0.1 123,969 - GFF818 0.57 +0.4 124,184 + GFF818 0.78 -0.5 124,184 + GFF818 0.78 -1.3 124,184 + GFF818 0.78 +0.4 124,184 + GFF818 0.78 -0.7 124,184 + GFF818 0.78 -0.8 124,184 + GFF818 0.78 -0.4 124,184 + GFF818 0.78 +0.9 124,184 + GFF818 0.78 -0.3 124,184 + GFF818 0.78 -0.4 124,184 + GFF818 0.78 +0.5 124,184 + GFF818 0.78 -0.2 124,185 - GFF818 0.78 -0.4 124,185 - GFF818 0.78 -0.6 124,185 - GFF818 0.78 +0.9 124,185 - GFF818 0.78 +0.4 124,185 - GFF818 0.78 +1.4 124,185 - GFF818 0.78 -2.3 124,185 - GFF818 0.78 -1.1 124,185 - GFF818 0.78 -1.5 124,185 - GFF818 0.78 +0.1 124,185 - GFF818 0.78 -0.1 124,185 - GFF818 0.78 +0.6 124,185 - GFF818 0.78 -0.2 124,585 + GFF819 0.33 -0.4 124,756 + GFF819 0.64 +0.1 124,757 - GFF819 0.64 +0.2 124,757 - GFF819 0.64 -0.6 124,987 - -0.4 124,987 - +0.2 124,987 - -0.2 124,987 - +0.1 125,022 + GFF820 0.11 -3.2 125,040 + GFF820 0.14 -0.6 125,040 + GFF820 0.14 +0.6 125,041 - GFF820 0.14 +0.4 125,041 - GFF820 0.14 -1.4 125,041 - GFF820 0.14 -1.9 125,043 + GFF820 0.15 -0.0 125,044 - GFF820 0.15 -0.5 125,044 - GFF820 0.15 -0.5 125,044 - GFF820 0.15 -0.3 125,394 + GFF820 0.69 -1.9 125,394 + GFF820 0.69 -0.7 125,394 + GFF820 0.69 -1.9 125,394 + GFF820 0.69 +0.2 125,394 + GFF820 0.69 -0.7 125,394 + GFF820 0.69 +0.8 125,395 - GFF820 0.69 -0.0 125,395 - GFF820 0.69 -0.8 125,395 - GFF820 0.69 +0.2 125,597 - -0.5 125,647 + -1.2 125,647 + -0.7 125,647 + -0.3 125,647 + +0.6 125,647 + -0.2 125,647 + -1.1 125,648 - -0.5 125,648 - +0.9 125,661 + +0.6 125,661 + -0.5 125,662 - -0.5 125,662 - -0.2 125,662 - -0.3 125,730 + -0.7 125,750 - -0.4 125,750 - -1.1 125,779 + -1.2 125,780 - -1.8 125,780 - -1.2 125,780 - -0.1 125,782 + -1.3 125,783 + +0.9 125,783 + -0.2 125,783 + +1.1 125,783 + -0.2 125,783 + +0.9 125,783 + -0.9 125,783 + -0.6 125,784 - -0.4 125,784 - +0.1 125,784 - +0.0 125,784 - +0.4 125,784 - +0.2 125,784 - -0.8 125,784 - +0.6 125,787 + +0.6 125,787 + -0.9 125,788 - -0.5 125,788 - -0.2 125,788 - +0.7 125,818 + -1.6 125,818 + -0.7 125,818 + -1.4 125,818 + -1.1 125,818 + -0.9 125,818 + +2.2 125,818 + +0.4 125,818 + -1.9 125,818 + -0.9 125,818 + -1.6 125,819 - -1.1 125,819 - -0.2 125,819 - -2.4 125,819 - -1.6 125,819 - +0.4 125,917 + GFF821 0.16 +2.4 126,253 + GFF821 0.61 +1.4 126,253 + GFF821 0.61 -0.6 126,253 + GFF821 0.61 +0.7 126,254 - GFF821 0.62 -0.4 126,254 - GFF821 0.62 -0.2 126,350 - GFF821 0.75 +0.0 126,487 + -0.9
Or see this region's nucleotide sequence