Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF7115 and GFF7116 are separated by 16 nucleotides GFF7116 and GFF7117 overlap by 4 nucleotides GFF7117 and GFF7118 overlap by 4 nucleotides GFF7118 and GFF7119 are separated by 6 nucleotides
GFF7115 - hypothetical protein, at 33,663 to 34,481
GFF7115
GFF7116 - 2,3-dihydroxybiphenyl 1,2-dioxygenase (EC 1.13.11.39), at 34,498 to 35,412
GFF7116
GFF7117 - 2-hydroxymuconic semialdehyde hydrolase (EC 3.7.1.9), at 35,409 to 36,239
GFF7117
GFF7118 - 4-hydroxythreonine-4-phosphate dehydrogenase (EC 1.1.1.262), at 36,236 to 37,177
GFF7118
GFF7119 - Ortho-halobenzoate 1,2-dioxygenase beta-ISP protein OhbA, at 37,184 to 37,660
GFF7119
Position (kb)
35
36
37 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 34.535 kb on + strand at 34.535 kb on + strand at 34.535 kb on + strand at 34.535 kb on + strand at 34.535 kb on + strand at 34.535 kb on + strand at 34.536 kb on - strand at 34.536 kb on - strand at 34.536 kb on - strand at 34.536 kb on - strand at 34.538 kb on + strand at 34.538 kb on + strand at 34.538 kb on + strand at 34.538 kb on + strand at 34.539 kb on - strand at 34.550 kb on + strand at 34.551 kb on - strand at 34.551 kb on - strand at 34.553 kb on + strand at 34.553 kb on + strand at 34.554 kb on - strand at 34.554 kb on - strand at 34.554 kb on - strand at 34.554 kb on - strand at 34.625 kb on + strand, within GFF7116 at 34.625 kb on + strand, within GFF7116 at 34.625 kb on + strand, within GFF7116 at 34.626 kb on - strand, within GFF7116 at 34.626 kb on - strand, within GFF7116 at 34.626 kb on - strand, within GFF7116 at 34.678 kb on + strand, within GFF7116 at 34.678 kb on + strand, within GFF7116 at 34.678 kb on + strand, within GFF7116 at 34.679 kb on - strand, within GFF7116 at 34.679 kb on - strand, within GFF7116 at 34.679 kb on - strand, within GFF7116 at 34.679 kb on - strand, within GFF7116 at 34.679 kb on - strand, within GFF7116 at 34.679 kb on - strand, within GFF7116 at 34.733 kb on + strand, within GFF7116 at 34.733 kb on + strand, within GFF7116 at 34.733 kb on + strand, within GFF7116 at 34.734 kb on - strand, within GFF7116 at 34.734 kb on - strand, within GFF7116 at 34.734 kb on - strand, within GFF7116 at 34.734 kb on - strand, within GFF7116 at 34.789 kb on + strand, within GFF7116 at 34.789 kb on + strand, within GFF7116 at 34.789 kb on + strand, within GFF7116 at 34.790 kb on - strand, within GFF7116 at 34.790 kb on - strand, within GFF7116 at 34.863 kb on - strand, within GFF7116 at 34.895 kb on + strand, within GFF7116 at 34.895 kb on + strand, within GFF7116 at 34.895 kb on + strand, within GFF7116 at 34.895 kb on + strand, within GFF7116 at 34.896 kb on - strand, within GFF7116 at 34.896 kb on - strand, within GFF7116 at 34.896 kb on - strand, within GFF7116 at 35.014 kb on + strand, within GFF7116 at 35.015 kb on - strand, within GFF7116 at 35.015 kb on - strand, within GFF7116 at 35.015 kb on - strand, within GFF7116 at 35.028 kb on - strand, within GFF7116 at 35.028 kb on - strand, within GFF7116 at 35.028 kb on - strand, within GFF7116 at 35.028 kb on - strand, within GFF7116 at 35.028 kb on - strand, within GFF7116 at 35.028 kb on - strand, within GFF7116 at 35.046 kb on + strand, within GFF7116 at 35.047 kb on - strand, within GFF7116 at 35.154 kb on + strand, within GFF7116 at 35.154 kb on + strand, within GFF7116 at 35.154 kb on + strand, within GFF7116 at 35.154 kb on + strand, within GFF7116 at 35.155 kb on - strand, within GFF7116 at 35.155 kb on - strand, within GFF7116 at 35.155 kb on - strand, within GFF7116 at 35.155 kb on - strand, within GFF7116 at 35.155 kb on - strand, within GFF7116 at 35.155 kb on - strand, within GFF7116 at 35.195 kb on + strand, within GFF7116 at 35.195 kb on + strand, within GFF7116 at 35.196 kb on - strand, within GFF7116 at 35.200 kb on + strand, within GFF7116 at 35.200 kb on + strand, within GFF7116 at 35.200 kb on + strand, within GFF7116 at 35.200 kb on + strand, within GFF7116 at 35.200 kb on + strand, within GFF7116 at 35.200 kb on + strand, within GFF7116 at 35.200 kb on + strand, within GFF7116 at 35.200 kb on + strand, within GFF7116 at 35.200 kb on + strand, within GFF7116 at 35.200 kb on + strand, within GFF7116 at 35.200 kb on + strand, within GFF7116 at 35.200 kb on + strand, within GFF7116 at 35.200 kb on + strand, within GFF7116 at 35.200 kb on + strand, within GFF7116 at 35.201 kb on - strand, within GFF7116 at 35.201 kb on - strand, within GFF7116 at 35.201 kb on - strand, within GFF7116 at 35.201 kb on - strand, within GFF7116 at 35.201 kb on - strand, within GFF7116 at 35.201 kb on - strand, within GFF7116 at 35.201 kb on - strand, within GFF7116 at 35.201 kb on - strand, within GFF7116 at 35.201 kb on - strand, within GFF7116 at 35.201 kb on - strand, within GFF7116 at 35.201 kb on - strand, within GFF7116 at 35.201 kb on - strand, within GFF7116 at 35.201 kb on - strand, within GFF7116 at 35.201 kb on - strand, within GFF7116 at 35.201 kb on - strand, within GFF7116 at 35.201 kb on - strand, within GFF7116 at 35.201 kb on - strand, within GFF7116 at 35.201 kb on - strand, within GFF7116 at 35.201 kb on - strand, within GFF7116 at 35.201 kb on - strand, within GFF7116 at 35.315 kb on + strand, within GFF7116 at 35.315 kb on + strand, within GFF7116 at 35.316 kb on - strand, within GFF7116 at 35.316 kb on - strand, within GFF7116 at 35.316 kb on - strand, within GFF7116 at 35.316 kb on - strand, within GFF7116 at 35.357 kb on + strand at 35.357 kb on + strand at 35.358 kb on - strand at 35.582 kb on + strand, within GFF7117 at 35.583 kb on + strand, within GFF7117 at 35.583 kb on + strand, within GFF7117 at 35.583 kb on + strand, within GFF7117 at 35.583 kb on + strand, within GFF7117 at 35.583 kb on + strand, within GFF7117 at 35.583 kb on + strand, within GFF7117 at 35.584 kb on - strand, within GFF7117 at 35.584 kb on - strand, within GFF7117 at 35.584 kb on - strand, within GFF7117 at 35.584 kb on - strand, within GFF7117 at 35.584 kb on - strand, within GFF7117 at 35.584 kb on - strand, within GFF7117 at 35.584 kb on - strand, within GFF7117 at 35.584 kb on - strand, within GFF7117 at 35.584 kb on - strand, within GFF7117 at 35.584 kb on - strand, within GFF7117 at 35.584 kb on - strand, within GFF7117 at 35.771 kb on + strand, within GFF7117 at 35.771 kb on + strand, within GFF7117 at 35.771 kb on + strand, within GFF7117 at 35.771 kb on + strand, within GFF7117 at 35.771 kb on + strand, within GFF7117 at 35.771 kb on + strand, within GFF7117 at 35.771 kb on + strand, within GFF7117 at 35.772 kb on - strand, within GFF7117 at 35.772 kb on - strand, within GFF7117 at 35.772 kb on - strand, within GFF7117 at 35.772 kb on - strand, within GFF7117 at 35.797 kb on + strand, within GFF7117 at 35.971 kb on + strand, within GFF7117 at 35.992 kb on + strand, within GFF7117 at 35.992 kb on + strand, within GFF7117 at 35.992 kb on + strand, within GFF7117 at 35.992 kb on + strand, within GFF7117 at 35.993 kb on - strand, within GFF7117 at 35.993 kb on - strand, within GFF7117 at 36.004 kb on + strand, within GFF7117 at 36.094 kb on + strand, within GFF7117 at 36.094 kb on + strand, within GFF7117 at 36.095 kb on - strand, within GFF7117 at 36.095 kb on - strand, within GFF7117 at 36.095 kb on - strand, within GFF7117 at 36.095 kb on - strand, within GFF7117 at 36.109 kb on + strand, within GFF7117 at 36.175 kb on + strand at 36.175 kb on + strand at 36.175 kb on + strand at 36.176 kb on - strand at 36.176 kb on - strand at 36.176 kb on - strand at 36.176 kb on - strand at 36.186 kb on + strand at 36.186 kb on + strand at 36.186 kb on + strand at 36.186 kb on + strand at 36.187 kb on - strand at 36.230 kb on + strand at 36.230 kb on + strand at 36.230 kb on + strand at 36.230 kb on + strand at 36.230 kb on + strand at 36.230 kb on + strand at 36.231 kb on - strand at 36.231 kb on - strand at 36.231 kb on - strand at 36.447 kb on + strand, within GFF7118 at 36.447 kb on + strand, within GFF7118 at 36.447 kb on + strand, within GFF7118 at 36.448 kb on - strand, within GFF7118 at 36.448 kb on - strand, within GFF7118 at 36.448 kb on - strand, within GFF7118 at 36.921 kb on - strand, within GFF7118 at 36.924 kb on + strand, within GFF7118 at 36.924 kb on + strand, within GFF7118 at 36.924 kb on + strand, within GFF7118 at 36.925 kb on - strand, within GFF7118 at 36.925 kb on - strand, within GFF7118 at 36.925 kb on - strand, within GFF7118 at 36.925 kb on - strand, within GFF7118 at 36.925 kb on - strand, within GFF7118 at 36.925 kb on - strand, within GFF7118 at 36.925 kb on - strand, within GFF7118 at 36.925 kb on - strand, within GFF7118 at 36.925 kb on - strand, within GFF7118 at 36.972 kb on + strand, within GFF7118 at 36.972 kb on + strand, within GFF7118 at 36.972 kb on + strand, within GFF7118 at 36.972 kb on + strand, within GFF7118 at 36.973 kb on - strand, within GFF7118 at 36.973 kb on - strand, within GFF7118 at 37.032 kb on + strand, within GFF7118 at 37.185 kb on + strand at 37.185 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 34,535 + +0.6 34,535 + -1.0 34,535 + -0.0 34,535 + -0.4 34,535 + -1.1 34,535 + +1.5 34,536 - -1.4 34,536 - +0.1 34,536 - +1.1 34,536 - +0.2 34,538 + -0.7 34,538 + +1.0 34,538 + -0.5 34,538 + -0.2 34,539 - -0.1 34,550 + -0.1 34,551 - +0.4 34,551 - +0.4 34,553 + +1.0 34,553 + -1.3 34,554 - +0.3 34,554 - +1.1 34,554 - +0.1 34,554 - +0.0 34,625 + GFF7116 0.14 -0.3 34,625 + GFF7116 0.14 -0.6 34,625 + GFF7116 0.14 -0.8 34,626 - GFF7116 0.14 -0.2 34,626 - GFF7116 0.14 +0.7 34,626 - GFF7116 0.14 +0.3 34,678 + GFF7116 0.20 +1.7 34,678 + GFF7116 0.20 -0.3 34,678 + GFF7116 0.20 -0.2 34,679 - GFF7116 0.20 -1.0 34,679 - GFF7116 0.20 -0.6 34,679 - GFF7116 0.20 +0.2 34,679 - GFF7116 0.20 +0.0 34,679 - GFF7116 0.20 -0.3 34,679 - GFF7116 0.20 +0.7 34,733 + GFF7116 0.26 +0.2 34,733 + GFF7116 0.26 +1.0 34,733 + GFF7116 0.26 -1.5 34,734 - GFF7116 0.26 -0.9 34,734 - GFF7116 0.26 -0.7 34,734 - GFF7116 0.26 +0.6 34,734 - GFF7116 0.26 -0.4 34,789 + GFF7116 0.32 +0.3 34,789 + GFF7116 0.32 +0.0 34,789 + GFF7116 0.32 -0.3 34,790 - GFF7116 0.32 +1.5 34,790 - GFF7116 0.32 -1.4 34,863 - GFF7116 0.40 -1.4 34,895 + GFF7116 0.43 -0.4 34,895 + GFF7116 0.43 -0.1 34,895 + GFF7116 0.43 +0.2 34,895 + GFF7116 0.43 -0.1 34,896 - GFF7116 0.43 +0.0 34,896 - GFF7116 0.43 -1.0 34,896 - GFF7116 0.43 -0.3 35,014 + GFF7116 0.56 +0.0 35,015 - GFF7116 0.57 +0.9 35,015 - GFF7116 0.57 -0.4 35,015 - GFF7116 0.57 +0.3 35,028 - GFF7116 0.58 -0.1 35,028 - GFF7116 0.58 +0.6 35,028 - GFF7116 0.58 -0.5 35,028 - GFF7116 0.58 +0.1 35,028 - GFF7116 0.58 +1.1 35,028 - GFF7116 0.58 +0.5 35,046 + GFF7116 0.60 -0.4 35,047 - GFF7116 0.60 -0.2 35,154 + GFF7116 0.72 +0.5 35,154 + GFF7116 0.72 -0.9 35,154 + GFF7116 0.72 -0.2 35,154 + GFF7116 0.72 +0.5 35,155 - GFF7116 0.72 -0.9 35,155 - GFF7116 0.72 +0.7 35,155 - GFF7116 0.72 -0.5 35,155 - GFF7116 0.72 -0.9 35,155 - GFF7116 0.72 -0.3 35,155 - GFF7116 0.72 +0.7 35,195 + GFF7116 0.76 -2.2 35,195 + GFF7116 0.76 -0.7 35,196 - GFF7116 0.76 -0.6 35,200 + GFF7116 0.77 -0.4 35,200 + GFF7116 0.77 -0.3 35,200 + GFF7116 0.77 -0.4 35,200 + GFF7116 0.77 -0.3 35,200 + GFF7116 0.77 -1.0 35,200 + GFF7116 0.77 -0.5 35,200 + GFF7116 0.77 -0.8 35,200 + GFF7116 0.77 -0.7 35,200 + GFF7116 0.77 -0.4 35,200 + GFF7116 0.77 +1.7 35,200 + GFF7116 0.77 -0.1 35,200 + GFF7116 0.77 -0.4 35,200 + GFF7116 0.77 -1.0 35,200 + GFF7116 0.77 -0.2 35,201 - GFF7116 0.77 -1.7 35,201 - GFF7116 0.77 +0.3 35,201 - GFF7116 0.77 +0.7 35,201 - GFF7116 0.77 -0.4 35,201 - GFF7116 0.77 +0.1 35,201 - GFF7116 0.77 -0.1 35,201 - GFF7116 0.77 -0.4 35,201 - GFF7116 0.77 +0.5 35,201 - GFF7116 0.77 -0.4 35,201 - GFF7116 0.77 -0.7 35,201 - GFF7116 0.77 +0.1 35,201 - GFF7116 0.77 -0.6 35,201 - GFF7116 0.77 +0.3 35,201 - GFF7116 0.77 -0.6 35,201 - GFF7116 0.77 -0.5 35,201 - GFF7116 0.77 +2.0 35,201 - GFF7116 0.77 -0.2 35,201 - GFF7116 0.77 -0.1 35,201 - GFF7116 0.77 +1.7 35,201 - GFF7116 0.77 -0.5 35,315 + GFF7116 0.89 -0.3 35,315 + GFF7116 0.89 -0.5 35,316 - GFF7116 0.89 -1.9 35,316 - GFF7116 0.89 +0.8 35,316 - GFF7116 0.89 -0.6 35,316 - GFF7116 0.89 -0.1 35,357 + +0.1 35,357 + -0.2 35,358 - -2.3 35,582 + GFF7117 0.21 -0.5 35,583 + GFF7117 0.21 +1.3 35,583 + GFF7117 0.21 -1.1 35,583 + GFF7117 0.21 +0.3 35,583 + GFF7117 0.21 -0.1 35,583 + GFF7117 0.21 +0.4 35,583 + GFF7117 0.21 +0.3 35,584 - GFF7117 0.21 +0.5 35,584 - GFF7117 0.21 -0.1 35,584 - GFF7117 0.21 -0.1 35,584 - GFF7117 0.21 +0.4 35,584 - GFF7117 0.21 +0.1 35,584 - GFF7117 0.21 -0.1 35,584 - GFF7117 0.21 -0.6 35,584 - GFF7117 0.21 -0.2 35,584 - GFF7117 0.21 -0.6 35,584 - GFF7117 0.21 +0.1 35,584 - GFF7117 0.21 +2.3 35,771 + GFF7117 0.44 +1.1 35,771 + GFF7117 0.44 +0.1 35,771 + GFF7117 0.44 +0.8 35,771 + GFF7117 0.44 -0.4 35,771 + GFF7117 0.44 +0.1 35,771 + GFF7117 0.44 -0.2 35,771 + GFF7117 0.44 +2.0 35,772 - GFF7117 0.44 -0.7 35,772 - GFF7117 0.44 -0.1 35,772 - GFF7117 0.44 +0.9 35,772 - GFF7117 0.44 +0.1 35,797 + GFF7117 0.47 +0.3 35,971 + GFF7117 0.68 +0.4 35,992 + GFF7117 0.70 +0.2 35,992 + GFF7117 0.70 -0.7 35,992 + GFF7117 0.70 +0.7 35,992 + GFF7117 0.70 -0.4 35,993 - GFF7117 0.70 -0.4 35,993 - GFF7117 0.70 -0.7 36,004 + GFF7117 0.72 -1.4 36,094 + GFF7117 0.82 -1.4 36,094 + GFF7117 0.82 -0.4 36,095 - GFF7117 0.83 +0.6 36,095 - GFF7117 0.83 -0.9 36,095 - GFF7117 0.83 -1.1 36,095 - GFF7117 0.83 +0.4 36,109 + GFF7117 0.84 +0.6 36,175 + -1.6 36,175 + -0.4 36,175 + +0.4 36,176 - -0.3 36,176 - -1.7 36,176 - +0.2 36,176 - -0.6 36,186 + -1.3 36,186 + -0.3 36,186 + +0.2 36,186 + -0.4 36,187 - +0.4 36,230 + -1.7 36,230 + +0.5 36,230 + -2.1 36,230 + +0.1 36,230 + -0.1 36,230 + +0.5 36,231 - -0.6 36,231 - -0.5 36,231 - -2.7 36,447 + GFF7118 0.22 +1.1 36,447 + GFF7118 0.22 +0.4 36,447 + GFF7118 0.22 +0.4 36,448 - GFF7118 0.23 +1.3 36,448 - GFF7118 0.23 +0.1 36,448 - GFF7118 0.23 -0.4 36,921 - GFF7118 0.73 -0.3 36,924 + GFF7118 0.73 -0.3 36,924 + GFF7118 0.73 +0.4 36,924 + GFF7118 0.73 -0.5 36,925 - GFF7118 0.73 -1.6 36,925 - GFF7118 0.73 +0.4 36,925 - GFF7118 0.73 -0.1 36,925 - GFF7118 0.73 +0.9 36,925 - GFF7118 0.73 -2.3 36,925 - GFF7118 0.73 -0.4 36,925 - GFF7118 0.73 +0.9 36,925 - GFF7118 0.73 +0.1 36,925 - GFF7118 0.73 -0.9 36,972 + GFF7118 0.78 +0.1 36,972 + GFF7118 0.78 +0.4 36,972 + GFF7118 0.78 -1.0 36,972 + GFF7118 0.78 -0.6 36,973 - GFF7118 0.78 -0.8 36,973 - GFF7118 0.78 -2.4 37,032 + GFF7118 0.85 +1.1 37,185 + +1.7 37,185 + +0.2
Or see this region's nucleotide sequence