Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6783 and GFF6784 are separated by 40 nucleotides GFF6784 and GFF6785 are separated by 24 nucleotides GFF6785 and GFF6786 are separated by 35 nucleotides GFF6786 and GFF6787 are separated by 13 nucleotides
GFF6783 - endo-type 6-aminohexanoate oligomer hydrolase, at 32,947 to 33,975
GFF6783
GFF6784 - D-amino acid dehydrogenase (EC 1.4.99.6), at 34,016 to 35,311
GFF6784
GFF6785 - Cobalt-zinc-cadmium resistance protein CzcA; Cation efflux system protein CusA, at 35,336 to 35,626
GFF6785
GFF6786 - CopG protein, at 35,662 to 36,123
GFF6786
GFF6787 - Copper tolerance protein, at 36,137 to 36,661
GFF6787
Position (kb) 34
35
36 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 33.572 kb on + strand, within GFF6783 at 33.986 kb on + strand at 34.003 kb on + strand at 34.003 kb on + strand at 34.003 kb on + strand at 34.004 kb on - strand at 34.004 kb on - strand at 34.178 kb on + strand, within GFF6784 at 34.178 kb on + strand, within GFF6784 at 34.179 kb on - strand, within GFF6784 at 34.299 kb on - strand, within GFF6784 at 34.299 kb on - strand, within GFF6784 at 34.331 kb on + strand, within GFF6784 at 34.481 kb on + strand, within GFF6784 at 34.527 kb on + strand, within GFF6784 at 34.527 kb on + strand, within GFF6784 at 34.607 kb on + strand, within GFF6784 at 34.607 kb on + strand, within GFF6784 at 34.608 kb on - strand, within GFF6784 at 34.662 kb on - strand, within GFF6784 at 35.310 kb on - strand at 35.643 kb on + strand at 35.643 kb on + strand at 35.643 kb on + strand at 35.644 kb on - strand at 35.644 kb on - strand at 35.720 kb on + strand, within GFF6786 at 35.721 kb on - strand, within GFF6786 at 35.741 kb on + strand, within GFF6786 at 35.742 kb on - strand, within GFF6786 at 35.865 kb on - strand, within GFF6786 at 36.198 kb on + strand, within GFF6787 at 36.198 kb on + strand, within GFF6787
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 33,572 + GFF6783 0.61 -2.8 33,986 + +0.7 34,003 + -1.6 34,003 + -0.2 34,003 + -0.0 34,004 - -0.6 34,004 - -0.4 34,178 + GFF6784 0.12 -0.9 34,178 + GFF6784 0.12 -0.4 34,179 - GFF6784 0.13 -0.1 34,299 - GFF6784 0.22 -0.9 34,299 - GFF6784 0.22 +0.2 34,331 + GFF6784 0.24 +0.2 34,481 + GFF6784 0.36 +0.4 34,527 + GFF6784 0.39 -1.9 34,527 + GFF6784 0.39 -0.1 34,607 + GFF6784 0.46 -0.0 34,607 + GFF6784 0.46 -0.3 34,608 - GFF6784 0.46 -1.5 34,662 - GFF6784 0.50 -1.8 35,310 - +0.8 35,643 + -2.0 35,643 + -0.0 35,643 + -0.4 35,644 - +1.2 35,644 - -1.4 35,720 + GFF6786 0.13 +2.4 35,721 - GFF6786 0.13 +1.4 35,741 + GFF6786 0.17 -0.8 35,742 - GFF6786 0.17 +0.1 35,865 - GFF6786 0.44 -0.3 36,198 + GFF6787 0.12 +0.1 36,198 + GFF6787 0.12 +0.4
Or see this region's nucleotide sequence