Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6193 and GFF6194 are separated by 46 nucleotides GFF6194 and GFF6195 overlap by 4 nucleotides GFF6195 and GFF6196 are separated by 7 nucleotides GFF6196 and GFF6197 overlap by 4 nucleotides
GFF6193 - ABC transporter, substrate-binding protein (cluster 5, nickel/peptides/opines), at 99,567 to 101,060
GFF6193
GFF6194 - ABC transporter, ATP-binding protein (cluster 5, nickel/peptides/opines), at 101,107 to 101,922
GFF6194
GFF6195 - ABC transporter, ATP-binding protein (cluster 5, nickel/peptides/opines), at 101,919 to 102,926
GFF6195
GFF6196 - ABC transporter, permease protein 2 (cluster 5, nickel/peptides/opines), at 102,934 to 103,815
GFF6196
GFF6197 - ABC transporter, permease protein 1 (cluster 5, nickel/peptides/opines), at 103,812 to 104,762
GFF6197
Position (kb) 101
102
103 Strain fitness (log2 ratio) -2
-1
0
1 at 101.066 kb on - strand at 101.153 kb on + strand at 101.153 kb on + strand at 101.154 kb on - strand at 101.154 kb on - strand at 101.280 kb on - strand, within GFF6194 at 101.280 kb on - strand, within GFF6194 at 101.280 kb on - strand, within GFF6194 at 101.280 kb on - strand, within GFF6194 at 101.598 kb on - strand, within GFF6194 at 101.861 kb on + strand at 102.247 kb on + strand, within GFF6195 at 102.247 kb on + strand, within GFF6195 at 102.247 kb on + strand, within GFF6195 at 102.247 kb on + strand, within GFF6195 at 102.247 kb on + strand, within GFF6195 at 102.248 kb on - strand, within GFF6195 at 103.532 kb on - strand, within GFF6196 at 103.816 kb on - strand at 103.816 kb on - strand at 103.816 kb on - strand at 103.816 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 101,066 - +0.5 101,153 + -1.4 101,153 + +0.5 101,154 - -2.0 101,154 - -1.3 101,280 - GFF6194 0.21 -0.0 101,280 - GFF6194 0.21 +0.8 101,280 - GFF6194 0.21 -1.2 101,280 - GFF6194 0.21 +0.8 101,598 - GFF6194 0.60 +0.2 101,861 + -0.6 102,247 + GFF6195 0.33 +0.2 102,247 + GFF6195 0.33 -1.1 102,247 + GFF6195 0.33 -2.1 102,247 + GFF6195 0.33 -2.4 102,247 + GFF6195 0.33 -0.9 102,248 - GFF6195 0.33 -1.1 103,532 - GFF6196 0.68 -0.9 103,816 - -1.9 103,816 - -1.5 103,816 - -1.7 103,816 - -1.1
Or see this region's nucleotide sequence