Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6111 and GFF6112 overlap by 4 nucleotides GFF6112 and GFF6113 are separated by 10 nucleotides GFF6113 and GFF6114 are separated by 19 nucleotides
GFF6111 - Oligopeptide ABC transporter, ATP-binding protein OppF (TC 3.A.1.5.1), at 10,167 to 11,165
GFF6111
GFF6112 - Oligopeptide ABC transporter, ATP-binding protein OppD (TC 3.A.1.5.1), at 11,162 to 12,193
GFF6112
GFF6113 - Dipeptide ABC transporter, permease protein DppC (TC 3.A.1.5.2), at 12,204 to 13,106
GFF6113
GFF6114 - ABC transporter, permease protein 1 (cluster 5, nickel/peptides/opines), at 13,126 to 14,061
GFF6114
Position (kb) 11
12
13 Strain fitness (log2 ratio) -2
-1
0
1 at 10.394 kb on - strand, within GFF6111 at 10.394 kb on - strand, within GFF6111 at 10.456 kb on + strand, within GFF6111 at 10.456 kb on + strand, within GFF6111 at 10.457 kb on - strand, within GFF6111 at 10.457 kb on - strand, within GFF6111 at 10.520 kb on - strand, within GFF6111 at 10.846 kb on + strand, within GFF6111 at 10.969 kb on - strand, within GFF6111 at 11.110 kb on + strand at 11.111 kb on - strand at 11.111 kb on - strand at 11.111 kb on - strand at 11.346 kb on - strand, within GFF6112 at 11.409 kb on + strand, within GFF6112 at 11.409 kb on + strand, within GFF6112 at 11.410 kb on - strand, within GFF6112 at 11.472 kb on + strand, within GFF6112 at 11.472 kb on + strand, within GFF6112 at 11.472 kb on + strand, within GFF6112 at 11.472 kb on + strand, within GFF6112 at 11.472 kb on + strand, within GFF6112 at 11.472 kb on + strand, within GFF6112 at 11.472 kb on + strand, within GFF6112 at 11.473 kb on - strand, within GFF6112 at 11.473 kb on - strand, within GFF6112 at 11.473 kb on - strand, within GFF6112 at 11.473 kb on - strand, within GFF6112 at 11.706 kb on + strand, within GFF6112 at 11.807 kb on + strand, within GFF6112 at 11.807 kb on + strand, within GFF6112 at 12.073 kb on - strand, within GFF6112 at 12.302 kb on - strand, within GFF6113 at 13.131 kb on - strand at 13.131 kb on - strand at 13.191 kb on - strand at 13.191 kb on - strand at 13.191 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 10,394 - GFF6111 0.23 -0.8 10,394 - GFF6111 0.23 -0.8 10,456 + GFF6111 0.29 +0.5 10,456 + GFF6111 0.29 -0.5 10,457 - GFF6111 0.29 +0.1 10,457 - GFF6111 0.29 +0.2 10,520 - GFF6111 0.35 +0.3 10,846 + GFF6111 0.68 -2.0 10,969 - GFF6111 0.80 -0.3 11,110 + +0.3 11,111 - -0.8 11,111 - -0.6 11,111 - +0.5 11,346 - GFF6112 0.18 -1.5 11,409 + GFF6112 0.24 -0.0 11,409 + GFF6112 0.24 -0.2 11,410 - GFF6112 0.24 -1.8 11,472 + GFF6112 0.30 +0.6 11,472 + GFF6112 0.30 -0.1 11,472 + GFF6112 0.30 -0.4 11,472 + GFF6112 0.30 -0.1 11,472 + GFF6112 0.30 +0.9 11,472 + GFF6112 0.30 +0.7 11,472 + GFF6112 0.30 +0.3 11,473 - GFF6112 0.30 -0.1 11,473 - GFF6112 0.30 -0.0 11,473 - GFF6112 0.30 +1.1 11,473 - GFF6112 0.30 -0.2 11,706 + GFF6112 0.53 +0.1 11,807 + GFF6112 0.62 -0.4 11,807 + GFF6112 0.62 -0.1 12,073 - GFF6112 0.88 -0.2 12,302 - GFF6113 0.11 -1.5 13,131 - -0.5 13,131 - +0.0 13,191 - -0.3 13,191 - +0.0 13,191 - -0.8
Or see this region's nucleotide sequence