Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6009 and GFF6010 are separated by 10 nucleotides GFF6010 and GFF6011 are separated by 6 nucleotides GFF6011 and GFF6012 are separated by 111 nucleotides
GFF6009 - Acyl-CoA dehydrogenase STM0857, at 68,617 to 69,780
GFF6009
GFF6010 - MmgE/PrpD family protein, at 69,791 to 71,152
GFF6010
GFF6011 - putative decarboxylase, at 71,159 to 71,866
GFF6011
GFF6012 - Tricarboxylate transport protein TctC, at 71,978 to 72,982
GFF6012
Position (kb)
69
70
71
72 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 68.887 kb on + strand, within GFF6009 at 68.887 kb on + strand, within GFF6009 at 68.887 kb on + strand, within GFF6009 at 68.887 kb on + strand, within GFF6009 at 68.888 kb on - strand, within GFF6009 at 68.888 kb on - strand, within GFF6009 at 68.888 kb on - strand, within GFF6009 at 68.938 kb on + strand, within GFF6009 at 69.055 kb on + strand, within GFF6009 at 69.325 kb on + strand, within GFF6009 at 69.325 kb on + strand, within GFF6009 at 69.325 kb on + strand, within GFF6009 at 69.326 kb on - strand, within GFF6009 at 69.326 kb on - strand, within GFF6009 at 69.416 kb on - strand, within GFF6009 at 69.524 kb on - strand, within GFF6009 at 69.586 kb on + strand, within GFF6009 at 69.586 kb on + strand, within GFF6009 at 69.587 kb on - strand, within GFF6009 at 69.701 kb on - strand at 69.701 kb on - strand at 69.739 kb on + strand at 69.739 kb on + strand at 69.743 kb on + strand at 69.744 kb on - strand at 69.999 kb on + strand, within GFF6010 at 70.000 kb on - strand, within GFF6010 at 70.000 kb on - strand, within GFF6010 at 70.000 kb on - strand, within GFF6010 at 70.242 kb on - strand, within GFF6010 at 70.242 kb on - strand, within GFF6010 at 70.598 kb on + strand, within GFF6010 at 70.805 kb on + strand, within GFF6010 at 70.805 kb on + strand, within GFF6010 at 70.914 kb on - strand, within GFF6010 at 71.057 kb on + strand at 71.057 kb on + strand at 71.058 kb on - strand at 71.122 kb on + strand at 71.463 kb on + strand, within GFF6011 at 71.497 kb on - strand, within GFF6011 at 71.522 kb on + strand, within GFF6011 at 71.523 kb on - strand, within GFF6011 at 71.731 kb on - strand, within GFF6011 at 71.873 kb on - strand at 71.998 kb on + strand at 71.998 kb on + strand at 71.998 kb on + strand at 71.999 kb on - strand at 72.075 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 68,887 + GFF6009 0.23 -0.0 68,887 + GFF6009 0.23 -0.0 68,887 + GFF6009 0.23 -2.2 68,887 + GFF6009 0.23 +0.3 68,888 - GFF6009 0.23 +0.6 68,888 - GFF6009 0.23 -0.6 68,888 - GFF6009 0.23 -0.3 68,938 + GFF6009 0.28 +0.6 69,055 + GFF6009 0.38 +1.1 69,325 + GFF6009 0.61 +0.5 69,325 + GFF6009 0.61 -0.2 69,325 + GFF6009 0.61 -0.9 69,326 - GFF6009 0.61 -0.9 69,326 - GFF6009 0.61 +0.4 69,416 - GFF6009 0.69 +0.6 69,524 - GFF6009 0.78 -0.4 69,586 + GFF6009 0.83 +0.6 69,586 + GFF6009 0.83 +0.2 69,587 - GFF6009 0.83 +0.0 69,701 - +0.2 69,701 - -0.8 69,739 + +0.9 69,739 + +2.6 69,743 + -0.4 69,744 - +0.4 69,999 + GFF6010 0.15 -0.8 70,000 - GFF6010 0.15 -0.8 70,000 - GFF6010 0.15 -0.1 70,000 - GFF6010 0.15 -0.2 70,242 - GFF6010 0.33 -2.6 70,242 - GFF6010 0.33 +1.1 70,598 + GFF6010 0.59 -0.1 70,805 + GFF6010 0.74 -0.2 70,805 + GFF6010 0.74 -0.9 70,914 - GFF6010 0.82 -1.0 71,057 + +1.0 71,057 + +0.0 71,058 - -0.2 71,122 + -0.4 71,463 + GFF6011 0.43 +0.7 71,497 - GFF6011 0.48 +0.2 71,522 + GFF6011 0.51 +0.0 71,523 - GFF6011 0.51 +0.7 71,731 - GFF6011 0.81 +0.6 71,873 - -0.5 71,998 + +2.0 71,998 + +0.1 71,998 + -1.9 71,999 - +0.1 72,075 + -1.5
Or see this region's nucleotide sequence