Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5853 and GFF5854 overlap by 4 nucleotides GFF5854 and GFF5855 are separated by 5 nucleotides
GFF5853 - Type I secretion system ATPase, LssB family LapB, at 32,166 to 34,292
GFF5853
GFF5854 - Type I secretion system, outer membrane component LapE, at 34,289 to 35,713
GFF5854
GFF5855 - T1SS secreted agglutinin RTX, at 35,719 to 55,560
GFF5855
Position (kb) 34
35
36 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 33.736 kb on + strand, within GFF5853 at 33.736 kb on + strand, within GFF5853 at 33.737 kb on - strand, within GFF5853 at 33.737 kb on - strand, within GFF5853 at 33.737 kb on - strand, within GFF5853 at 33.871 kb on + strand, within GFF5853 at 33.871 kb on + strand, within GFF5853 at 33.872 kb on - strand, within GFF5853 at 33.872 kb on - strand, within GFF5853 at 33.872 kb on - strand, within GFF5853 at 33.872 kb on - strand, within GFF5853 at 33.872 kb on - strand, within GFF5853 at 34.264 kb on + strand at 34.335 kb on + strand at 34.335 kb on + strand at 34.368 kb on + strand at 34.428 kb on + strand at 34.429 kb on - strand at 34.429 kb on - strand at 34.429 kb on - strand at 34.429 kb on - strand at 34.429 kb on - strand at 34.482 kb on + strand, within GFF5854 at 34.482 kb on + strand, within GFF5854 at 34.482 kb on + strand, within GFF5854 at 34.689 kb on + strand, within GFF5854 at 34.690 kb on - strand, within GFF5854 at 34.690 kb on - strand, within GFF5854 at 34.690 kb on - strand, within GFF5854 at 34.690 kb on - strand, within GFF5854 at 34.726 kb on - strand, within GFF5854 at 34.726 kb on - strand, within GFF5854 at 34.726 kb on - strand, within GFF5854 at 35.413 kb on - strand, within GFF5854 at 35.415 kb on + strand, within GFF5854 at 35.701 kb on + strand at 35.701 kb on + strand at 35.702 kb on - strand at 35.873 kb on + strand at 35.873 kb on + strand at 35.874 kb on - strand at 35.874 kb on - strand at 35.907 kb on - strand at 36.035 kb on + strand at 36.035 kb on + strand at 36.035 kb on + strand at 36.036 kb on - strand at 36.036 kb on - strand at 36.335 kb on + strand at 36.335 kb on + strand at 36.335 kb on + strand at 36.335 kb on + strand at 36.336 kb on - strand at 36.336 kb on - strand at 36.371 kb on + strand at 36.371 kb on + strand at 36.371 kb on + strand at 36.371 kb on + strand at 36.371 kb on + strand at 36.372 kb on - strand at 36.372 kb on - strand at 36.372 kb on - strand at 36.372 kb on - strand at 36.416 kb on + strand at 36.554 kb on + strand at 36.555 kb on - strand at 36.555 kb on - strand at 36.590 kb on + strand at 36.590 kb on + strand at 36.590 kb on + strand at 36.590 kb on + strand at 36.590 kb on + strand at 36.590 kb on + strand at 36.591 kb on - strand at 36.593 kb on + strand at 36.593 kb on + strand at 36.594 kb on - strand at 36.594 kb on - strand at 36.594 kb on - strand at 36.594 kb on - strand at 36.594 kb on - strand at 36.594 kb on - strand at 36.594 kb on - strand at 36.594 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 33,736 + GFF5853 0.74 +3.0 33,736 + GFF5853 0.74 +0.4 33,737 - GFF5853 0.74 +0.5 33,737 - GFF5853 0.74 -0.1 33,737 - GFF5853 0.74 +0.6 33,871 + GFF5853 0.80 +0.1 33,871 + GFF5853 0.80 -0.5 33,872 - GFF5853 0.80 +0.2 33,872 - GFF5853 0.80 +0.1 33,872 - GFF5853 0.80 -1.2 33,872 - GFF5853 0.80 -0.8 33,872 - GFF5853 0.80 -2.5 34,264 + +0.4 34,335 + -0.1 34,335 + -0.7 34,368 + -1.0 34,428 + +0.1 34,429 - -0.4 34,429 - -0.3 34,429 - -1.2 34,429 - -0.4 34,429 - -0.3 34,482 + GFF5854 0.14 -1.1 34,482 + GFF5854 0.14 +0.3 34,482 + GFF5854 0.14 -0.2 34,689 + GFF5854 0.28 -0.9 34,690 - GFF5854 0.28 -1.2 34,690 - GFF5854 0.28 -0.2 34,690 - GFF5854 0.28 +0.2 34,690 - GFF5854 0.28 +1.7 34,726 - GFF5854 0.31 +0.0 34,726 - GFF5854 0.31 -0.0 34,726 - GFF5854 0.31 +0.8 35,413 - GFF5854 0.79 +0.7 35,415 + GFF5854 0.79 -0.6 35,701 + +0.6 35,701 + +0.6 35,702 - +0.1 35,873 + -0.4 35,873 + -1.4 35,874 - +0.1 35,874 - +0.8 35,907 - -0.3 36,035 + +0.1 36,035 + +0.5 36,035 + -2.4 36,036 - +0.4 36,036 - +0.1 36,335 + +0.6 36,335 + +1.3 36,335 + +0.3 36,335 + +0.5 36,336 - +0.2 36,336 - +0.1 36,371 + +0.5 36,371 + -0.0 36,371 + +0.0 36,371 + -0.4 36,371 + -0.3 36,372 - +0.1 36,372 - -0.6 36,372 - +0.0 36,372 - -0.2 36,416 + +0.2 36,554 + -0.2 36,555 - -0.2 36,555 - +0.2 36,590 + -0.6 36,590 + +1.1 36,590 + -0.3 36,590 + -0.4 36,590 + -0.5 36,590 + +0.9 36,591 - -0.6 36,593 + -0.8 36,593 + -0.9 36,594 - +0.8 36,594 - +0.1 36,594 - +0.2 36,594 - -1.0 36,594 - -0.8 36,594 - -1.0 36,594 - -0.4 36,594 - +1.1
Or see this region's nucleotide sequence