Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5811 and GFF5812 are separated by 18 nucleotides GFF5812 and GFF5813 overlap by 4 nucleotides GFF5813 and GFF5814 are separated by 116 nucleotides
GFF5811 - Multidrug efflux system MdtABC-TolC, inner-membrane proton/drug antiporter MdtB-like, at 187,702 to 190,872
GFF5811
GFF5812 - DNA-binding heavy metal response regulator, at 190,891 to 191,631
GFF5812
GFF5813 - Heavy metal sensor histidine kinase, at 191,628 to 193,025
GFF5813
GFF5814 - Rhodanese-related sulfurtransferase, at 193,142 to 194,080
GFF5814
Position (kb) 191
192
193
194 Strain fitness (log2 ratio) -2
-1
0
1 at 190.763 kb on - strand at 190.763 kb on - strand at 190.763 kb on - strand at 190.763 kb on - strand at 190.768 kb on + strand at 190.768 kb on + strand at 190.769 kb on - strand at 190.769 kb on - strand at 190.792 kb on + strand at 190.966 kb on + strand, within GFF5812 at 191.573 kb on - strand at 191.748 kb on + strand at 192.637 kb on + strand, within GFF5813 at 192.637 kb on + strand, within GFF5813 at 192.638 kb on - strand, within GFF5813 at 192.977 kb on + strand at 192.977 kb on + strand at 193.712 kb on + strand, within GFF5814 at 193.943 kb on + strand, within GFF5814 at 193.943 kb on + strand, within GFF5814 at 193.944 kb on - strand, within GFF5814 at 193.944 kb on - strand, within GFF5814
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 190,763 - +1.6 190,763 - +0.4 190,763 - +0.1 190,763 - +0.2 190,768 + -1.2 190,768 + -0.6 190,769 - +0.9 190,769 - -0.3 190,792 + -1.0 190,966 + GFF5812 0.10 -0.4 191,573 - -0.8 191,748 + +1.2 192,637 + GFF5813 0.72 -0.2 192,637 + GFF5813 0.72 -0.4 192,638 - GFF5813 0.72 -1.8 192,977 + +0.4 192,977 + -1.5 193,712 + GFF5814 0.61 -0.2 193,943 + GFF5814 0.85 -0.7 193,943 + GFF5814 0.85 -0.8 193,944 - GFF5814 0.85 -0.7 193,944 - GFF5814 0.85 -0.9
Or see this region's nucleotide sequence