Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5803 and GFF5804 are separated by 130 nucleotides GFF5804 and GFF5805 are separated by 27 nucleotides GFF5805 and GFF5806 overlap by 14 nucleotides
GFF5803 - Urea ABC transporter, ATPase protein UrtE, at 178,928 to 179,620
GFF5803
GFF5804 - FIG00978734: hypothetical protein, at 179,751 to 181,004
GFF5804
GFF5805 - Putative transmembrane protein, at 181,032 to 181,718
GFF5805
GFF5806 - RNA polymerase ECF-type sigma factor, at 181,705 to 182,280
GFF5806
Position (kb) 179
180
181
182 Strain fitness (log2 ratio) -2
-1
0
1
2 at 178.845 kb on + strand at 178.956 kb on - strand at 178.956 kb on - strand at 178.959 kb on - strand at 179.040 kb on + strand, within GFF5803 at 179.234 kb on + strand, within GFF5803 at 179.234 kb on + strand, within GFF5803 at 179.234 kb on + strand, within GFF5803 at 179.235 kb on - strand, within GFF5803 at 179.271 kb on - strand, within GFF5803 at 179.271 kb on - strand, within GFF5803 at 179.271 kb on - strand, within GFF5803 at 179.498 kb on + strand, within GFF5803 at 179.501 kb on + strand, within GFF5803 at 179.531 kb on + strand, within GFF5803 at 179.653 kb on + strand at 179.653 kb on + strand at 179.653 kb on + strand at 179.653 kb on + strand at 179.654 kb on - strand at 179.654 kb on - strand at 179.654 kb on - strand at 179.701 kb on + strand at 179.970 kb on + strand, within GFF5804 at 179.970 kb on + strand, within GFF5804 at 180.102 kb on + strand, within GFF5804 at 180.102 kb on + strand, within GFF5804 at 180.102 kb on + strand, within GFF5804 at 180.102 kb on + strand, within GFF5804 at 180.102 kb on + strand, within GFF5804 at 180.102 kb on + strand, within GFF5804 at 180.103 kb on - strand, within GFF5804 at 180.153 kb on + strand, within GFF5804 at 180.153 kb on + strand, within GFF5804 at 180.153 kb on + strand, within GFF5804 at 180.153 kb on + strand, within GFF5804 at 180.153 kb on + strand, within GFF5804 at 180.154 kb on - strand, within GFF5804 at 180.154 kb on - strand, within GFF5804 at 180.154 kb on - strand, within GFF5804 at 180.154 kb on - strand, within GFF5804 at 180.154 kb on - strand, within GFF5804 at 180.492 kb on + strand, within GFF5804 at 180.493 kb on - strand, within GFF5804 at 180.493 kb on - strand, within GFF5804 at 180.493 kb on - strand, within GFF5804 at 180.648 kb on + strand, within GFF5804 at 181.033 kb on + strand at 181.162 kb on + strand, within GFF5805 at 181.162 kb on + strand, within GFF5805 at 181.162 kb on + strand, within GFF5805 at 181.258 kb on + strand, within GFF5805 at 181.291 kb on + strand, within GFF5805 at 181.291 kb on + strand, within GFF5805 at 181.291 kb on + strand, within GFF5805 at 181.292 kb on - strand, within GFF5805 at 181.292 kb on - strand, within GFF5805 at 181.889 kb on + strand, within GFF5806 at 181.890 kb on - strand, within GFF5806
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 178,845 + +1.4 178,956 - +0.8 178,956 - -0.9 178,959 - -0.8 179,040 + GFF5803 0.16 -0.5 179,234 + GFF5803 0.44 -0.7 179,234 + GFF5803 0.44 -0.3 179,234 + GFF5803 0.44 -0.7 179,235 - GFF5803 0.44 -2.6 179,271 - GFF5803 0.49 -1.2 179,271 - GFF5803 0.49 -0.8 179,271 - GFF5803 0.49 +0.2 179,498 + GFF5803 0.82 +0.1 179,501 + GFF5803 0.83 +1.0 179,531 + GFF5803 0.87 -1.2 179,653 + +0.3 179,653 + +0.4 179,653 + -0.0 179,653 + +0.6 179,654 - -1.1 179,654 - -0.4 179,654 - -0.9 179,701 + +1.6 179,970 + GFF5804 0.17 -0.3 179,970 + GFF5804 0.17 -0.9 180,102 + GFF5804 0.28 -2.0 180,102 + GFF5804 0.28 -0.1 180,102 + GFF5804 0.28 +0.7 180,102 + GFF5804 0.28 +1.2 180,102 + GFF5804 0.28 -1.0 180,102 + GFF5804 0.28 -0.0 180,103 - GFF5804 0.28 -1.3 180,153 + GFF5804 0.32 -0.0 180,153 + GFF5804 0.32 -0.2 180,153 + GFF5804 0.32 -0.1 180,153 + GFF5804 0.32 -1.7 180,153 + GFF5804 0.32 +0.1 180,154 - GFF5804 0.32 -1.6 180,154 - GFF5804 0.32 -0.9 180,154 - GFF5804 0.32 -0.4 180,154 - GFF5804 0.32 +0.5 180,154 - GFF5804 0.32 +0.2 180,492 + GFF5804 0.59 -1.1 180,493 - GFF5804 0.59 -0.2 180,493 - GFF5804 0.59 +0.2 180,493 - GFF5804 0.59 -1.2 180,648 + GFF5804 0.72 -1.3 181,033 + -0.4 181,162 + GFF5805 0.19 -0.2 181,162 + GFF5805 0.19 +0.3 181,162 + GFF5805 0.19 -0.7 181,258 + GFF5805 0.33 -1.3 181,291 + GFF5805 0.38 +2.6 181,291 + GFF5805 0.38 +0.1 181,291 + GFF5805 0.38 -0.0 181,292 - GFF5805 0.38 +0.3 181,292 - GFF5805 0.38 -1.4 181,889 + GFF5806 0.32 -0.0 181,890 - GFF5806 0.32 +0.6
Or see this region's nucleotide sequence