Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5768 and GFF5769 are separated by 1 nucleotides GFF5769 and GFF5770 are separated by 147 nucleotides GFF5770 and GFF5771 overlap by 4 nucleotides
GFF5768 - Chemotaxis regulator - transmits chemoreceptor signals to flagellar motor components CheY, at 140,724 to 141,122
GFF5768
GFF5769 - Chemotaxis response - phosphatase CheZ, at 141,124 to 141,768
GFF5769
GFF5770 - Flagellar biosynthesis protein FlhB, at 141,916 to 143,070
GFF5770
GFF5771 - Flagellar biosynthesis protein FlhA, at 143,067 to 145,166
GFF5771
Position (kb)
141
142
143
144 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 141.298 kb on + strand, within GFF5769 at 141.299 kb on - strand, within GFF5769 at 141.479 kb on - strand, within GFF5769 at 141.766 kb on + strand at 141.833 kb on - strand at 141.887 kb on + strand at 141.888 kb on - strand at 141.888 kb on - strand at 141.888 kb on - strand at 142.456 kb on + strand, within GFF5770 at 142.456 kb on + strand, within GFF5770 at 142.457 kb on - strand, within GFF5770 at 142.457 kb on - strand, within GFF5770 at 142.561 kb on + strand, within GFF5770 at 142.562 kb on - strand, within GFF5770 at 142.562 kb on - strand, within GFF5770 at 142.750 kb on + strand, within GFF5770 at 142.750 kb on + strand, within GFF5770 at 142.751 kb on - strand, within GFF5770 at 142.882 kb on + strand, within GFF5770 at 142.958 kb on - strand at 143.002 kb on - strand at 143.038 kb on + strand at 143.038 kb on + strand at 143.039 kb on - strand at 143.039 kb on - strand at 143.039 kb on - strand at 143.259 kb on + strand at 143.259 kb on + strand at 143.259 kb on + strand at 143.259 kb on + strand at 143.259 kb on + strand at 143.260 kb on - strand at 143.260 kb on - strand at 143.260 kb on - strand at 143.260 kb on - strand at 143.260 kb on - strand at 143.260 kb on - strand at 143.653 kb on - strand, within GFF5771 at 143.793 kb on + strand, within GFF5771 at 143.793 kb on + strand, within GFF5771 at 143.793 kb on + strand, within GFF5771 at 143.794 kb on - strand, within GFF5771
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 141,298 + GFF5769 0.27 +0.1 141,299 - GFF5769 0.27 -0.3 141,479 - GFF5769 0.55 -1.9 141,766 + -1.0 141,833 - -1.4 141,887 + -0.6 141,888 - +0.1 141,888 - +0.6 141,888 - +1.2 142,456 + GFF5770 0.47 +0.9 142,456 + GFF5770 0.47 -0.0 142,457 - GFF5770 0.47 +0.6 142,457 - GFF5770 0.47 -1.8 142,561 + GFF5770 0.56 -0.9 142,562 - GFF5770 0.56 -0.2 142,562 - GFF5770 0.56 -0.9 142,750 + GFF5770 0.72 -0.7 142,750 + GFF5770 0.72 -1.3 142,751 - GFF5770 0.72 -0.3 142,882 + GFF5770 0.84 -0.5 142,958 - +0.4 143,002 - -1.9 143,038 + +0.2 143,038 + -0.4 143,039 - +0.4 143,039 - +1.1 143,039 - -0.4 143,259 + -0.9 143,259 + -0.2 143,259 + +0.6 143,259 + -0.1 143,259 + -0.3 143,260 - +0.2 143,260 - -0.8 143,260 - -0.8 143,260 - -1.2 143,260 - -1.6 143,260 - -1.2 143,653 - GFF5771 0.28 -0.1 143,793 + GFF5771 0.35 -0.8 143,793 + GFF5771 0.35 +0.5 143,793 + GFF5771 0.35 +1.4 143,794 - GFF5771 0.35 +3.0
Or see this region's nucleotide sequence