Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5736 and GFF5737 are separated by 83 nucleotides GFF5737 and GFF5738 overlap by 4 nucleotides GFF5738 and GFF5739 overlap by 4 nucleotides
GFF5736 - Flagellar hook-associated protein FlgL, at 110,545 to 111,756
GFF5736
GFF5737 - 2-keto-3-deoxy-D-arabino-heptulosonate-7-phosphat e synthase I alpha (EC 2.5.1.54), at 111,840 to 112,964
GFF5737
GFF5738 - Flagellar biosynthesis protein FliR, at 112,961 to 113,758
GFF5738
GFF5739 - Flagellar biosynthesis protein FliQ, at 113,755 to 114,024
GFF5739
Position (kb) 111
112
113 Strain fitness (log2 ratio) -1
0
1
2 at 111.010 kb on + strand, within GFF5736 at 111.011 kb on - strand, within GFF5736 at 111.011 kb on - strand, within GFF5736 at 111.011 kb on - strand, within GFF5736 at 111.011 kb on - strand, within GFF5736 at 111.130 kb on - strand, within GFF5736 at 111.238 kb on + strand, within GFF5736 at 111.238 kb on + strand, within GFF5736 at 111.239 kb on - strand, within GFF5736 at 111.239 kb on - strand, within GFF5736 at 111.289 kb on + strand, within GFF5736 at 111.290 kb on - strand, within GFF5736 at 111.625 kb on + strand, within GFF5736 at 111.626 kb on - strand, within GFF5736 at 111.626 kb on - strand, within GFF5736 at 111.626 kb on - strand, within GFF5736 at 111.749 kb on - strand at 112.219 kb on - strand, within GFF5737 at 112.219 kb on - strand, within GFF5737 at 113.403 kb on + strand, within GFF5738 at 113.442 kb on + strand, within GFF5738 at 113.443 kb on - strand, within GFF5738 at 113.798 kb on + strand, within GFF5739 at 113.799 kb on - strand, within GFF5739
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 111,010 + GFF5736 0.38 +0.6 111,011 - GFF5736 0.38 -0.5 111,011 - GFF5736 0.38 +0.2 111,011 - GFF5736 0.38 +0.9 111,011 - GFF5736 0.38 -1.2 111,130 - GFF5736 0.48 -0.7 111,238 + GFF5736 0.57 +0.4 111,238 + GFF5736 0.57 -0.3 111,239 - GFF5736 0.57 +0.7 111,239 - GFF5736 0.57 -0.3 111,289 + GFF5736 0.61 +0.6 111,290 - GFF5736 0.61 +2.0 111,625 + GFF5736 0.89 +0.0 111,626 - GFF5736 0.89 -0.3 111,626 - GFF5736 0.89 -0.4 111,626 - GFF5736 0.89 -0.2 111,749 - +0.1 112,219 - GFF5737 0.34 -1.6 112,219 - GFF5737 0.34 -0.8 113,403 + GFF5738 0.55 -1.2 113,442 + GFF5738 0.60 -0.2 113,443 - GFF5738 0.60 -0.4 113,798 + GFF5739 0.16 +0.1 113,799 - GFF5739 0.16 -0.8
Or see this region's nucleotide sequence