Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5729 and GFF5730 are separated by 20 nucleotides GFF5730 and GFF5731 are separated by 67 nucleotides GFF5731 and GFF5732 are separated by 14 nucleotides
GFF5729 - Methyl-accepting chemotaxis sensor/transducer protein, at 99,299 to 100,903
GFF5729
GFF5730 - Positive regulator of CheA protein activity (CheW), at 100,924 to 101,445
GFF5730
GFF5731 - Methyl-accepting chemotaxis sensor/transducer protein, at 101,513 to 103,114
GFF5731
GFF5732 - Methyl-accepting chemotaxis sensor/transducer protein, at 103,129 to 104,832
GFF5732
Position (kb)
101
102
103
104 Strain fitness (log2 ratio)
-1
0
1 at 101.690 kb on + strand, within GFF5731 at 101.691 kb on - strand, within GFF5731 at 101.691 kb on - strand, within GFF5731 at 101.888 kb on + strand, within GFF5731 at 101.990 kb on + strand, within GFF5731 at 101.990 kb on + strand, within GFF5731 at 103.112 kb on + strand at 103.321 kb on + strand, within GFF5732 at 103.321 kb on + strand, within GFF5732 at 103.321 kb on + strand, within GFF5732 at 103.703 kb on - strand, within GFF5732 at 103.819 kb on + strand, within GFF5732
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 101,690 + GFF5731 0.11 -0.7 101,691 - GFF5731 0.11 +0.2 101,691 - GFF5731 0.11 +0.2 101,888 + GFF5731 0.23 +0.1 101,990 + GFF5731 0.30 -0.5 101,990 + GFF5731 0.30 -1.3 103,112 + -0.9 103,321 + GFF5732 0.11 -1.3 103,321 + GFF5732 0.11 -1.0 103,321 + GFF5732 0.11 +0.1 103,703 - GFF5732 0.34 -0.7 103,819 + GFF5732 0.40 -0.3
Or see this region's nucleotide sequence